Synthesis of a novel analytical reagent for the determination of active sites for conjugation on a catalytic aldolase monoclonal antibody

1,3-Diketones. Synthesis and properties

“…A multistep synthesis of analytical reagent 142 containing a β-dicarbonyl fragment was developed by Magano et al [93]. The required β-diketone with a 4-nitrophenyl substituent was prepared from acetylacetone or tert-butyl acetoacetate (Scheme 88).…”