Synthesis of a grapevine phytoalexin in transgenic tomatoes (Lycopersicon esculentumMill.) conditions resistance againstPhytophthora infestans

Thomzik, J. E.; Stenzel, K.; Stöcker, Ronald; Schreier, Peter; Hain, Rüdiger; Stahl, Dietmar J.

doi:10.1006/pmpp.1997.0123

Cited by 135 publications

(68 citation statements)

References 25 publications

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“…Indeed, transgenic expression of a STS gene from grapevine in various plant species, including tobacco (Nicotiana tabacum), tomato (Solanum lycopersicum), papaya (Carica papaya), and grapevine, has been shown to result in resveratrol and resveratrol derivatives accumulation (Hain et al, 1993;Thomzik et al, 1997;Hipskind and Paiva, 2000;Coutos-Thévenot et al, 2001). In a previous work, we have used Agrobacterium tumefaciens-mediated transient transformation of Nicotiana benthamiana to characterize the activity of a resveratrol O-methyltransferase from grapevine (Schmidlin et al, 2008); we thus chose the same approach here to investigate the activity of a selection of STS proteins in planta.…”

Section: Functional Characterization Of a Selection Of Sts Proteinsmentioning

confidence: 99%

Structural, Functional, and Evolutionary Analysis of the Unusually Large Stilbene Synthase Gene Family in Grapevine

et al. 2012

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de Ciência, P-2780-156 Oeiras, Portugal (R.T., G.A.B.M.)Stilbenes are a small family of phenylpropanoids produced in a number of unrelated plant species, including grapevine (Vitis vinifera). In addition to their participation in defense mechanisms in plants, stilbenes, such as resveratrol, display important pharmacological properties and are postulated to be involved in the health benefits associated with a moderate consumption of red wine. Stilbene synthases (STSs), which catalyze the biosynthesis of the stilbene backbone, seem to have evolved from chalcone synthases (CHSs) several times independently in stilbene-producing plants. STS genes usually form small families of two to five closely related paralogs. By contrast, the sequence of grapevine reference genome (cv PN40024) has revealed an unusually large STS gene family. Here, we combine molecular evolution and structural and functional analyses to investigate further the high number of STS genes in grapevine. Our reannotation of the STS and CHS gene families yielded 48 STS genes, including at least 32 potentially functional ones. Functional characterization of nine genes representing most of the STS gene family diversity clearly indicated that these genes do encode for proteins with STS activity. Evolutionary analysis of the STS gene family revealed that both STS and CHS evolution are dominated by purifying selection, with no evidence for strong selection for new functions among STS genes. However, we found a few sites under different selection pressures in CHS and STS sequences, whose potential functional consequences are discussed using a structural model of a typical STS from grapevine that we developed.

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Section: Functional Characterization Of a Selection Of Sts Proteinsmentioning

confidence: 99%

Structural, Functional, and Evolutionary Analysis of the Unusually Large Stilbene Synthase Gene Family in Grapevine

et al. 2012

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“…Transgenic expression of VST1 in various plant species, including tobacco (Nicotiana spp. ), tomato (Solanum lycopersicum), papaya (Carica papaya), and grapevine, has been shown to result in resveratrol and resveratrol derivative accumulation in transformed plants (Hain et al, 1990;Thomzik et al, 1997;Hipskind and Paiva, 2000;Coutos-Thévenot et al, 2001). As a control, tobacco leaves were infiltrated with Agrobacterium harboring a 35S-GFP construct (Haseloff et al, 1997).…”

Section: Characterization Of Romt Activity In Plantamentioning

confidence: 99%

A Stress-Inducible Resveratrol O-Methyltransferase Involved in the Biosynthesis of Pterostilbene in Grapevine

et al. 2008

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Stilbenes are considered the most important phytoalexin group in grapevine (Vitis vinifera) and they are known to contribute to the protection against various pathogens. The main stilbenes in grapevine are resveratrol and its derivatives and, among these, pterostilbene has recently attracted much attention due both to its antifungal and pharmacological properties. Indeed, pterostilbene is 5 to 10 times more fungitoxic than resveratrol in vitro and recent studies have shown that pterostilbene exhibits anticancer, hypolipidemic, and antidiabetic properties. A candidate gene approach was used to identify a grapevine resveratrol O-methyltransferase (ROMT) cDNA and the activity of the corresponding protein was characterized after expression in Escherichia coli. Transient coexpression of ROMT and grapevine stilbene synthase in tobacco (Nicotiana benthamiana) using the agroinfiltration technique resulted in the accumulation of pterostilbene in tobacco tissues. Taken together, these results showed that ROMT was able to catalyze the biosynthesis of pterostilbene from resveratrol both in vitro and in planta. ROMT gene expression in grapevine leaves was induced by different stresses, including downy mildew (Plasmopara viticola) infection, ultraviolet light, and AlCl 3 treatment.

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“…Thomzik et al (1997) transferred two genes from the grapevine coding for stilbene synthase into tomato. The accumulation of phytoalexin trans-resveratrol was detectable after fungal inoculation and resulted in a significant increase in the resistance of transgenic tomato to Phytophthora infestans but no significant increase in resistance to Botrytis cinerea and Alternaria solani.…”

Section: Discussionmentioning

confidence: 99%

“…First, a constitutive CaMV35S promoter triggered strong and constitutive stilbene accumulation but caused a drastic depletion of the endogenous precursor pools. This affects stilbene production and may interfere with secondary biosynthetic pathways (Hain et al 1990(Hain et al , 1993Thomzik et al 1997). Second, these GM plants may produce resveratrol glucoside (piceid), which has little or no antifungal activities compared with resveratrol and its dehydrodimer δ-viniferin, which are more effective against fungus (P. viticola) zoospores in grapevines (Kobayashi et al 2000;Giorcelli et al 2004;Chang et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Bioassay for the Response of Resveratrol Transgenic Rice Lines to Bacterial and Fungal Diseases

Yang

Kim²,

Ahn

et al. 2013

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Resveratrol is a stilbenoid and phytoalexin produced in response to stresses, such as wounding, and pathogen attacks by bacteria or fungi. Two resveratrol rice lines, Iksan515 and Iksan526, were used to examine resistance against Korean pathogen races for bacterial blight, leaf blast, and brown leaf spot. The screening test for bacterial blight demonstrated an increased susceptibility of both transgenic lines to K1 race, and a more susceptible Iksan515 to K2. Phenotypic evaluation for resistance to brown leaf spot also revealed the susceptibility of Iksan526 to the disease which did not significantly differ from the isogenic variety 'Dongjin', and a slightly more susceptible Iksan515 to the disease compared to check. When the lines were screened with three races (KJ301, KJ101 and KJ133) of leaf blasts in the field, both transgenic lines exhibited resistance but at the same level with 'Dongjin'. Our overall findings suggest that foreign phytoalexin resveratrol production in transgenic rice lines transformed with resveratrol synthase gene did not confer resistance against fungal and bacterial diseases.

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Synthesis of a grapevine phytoalexin in transgenic tomatoes (Lycopersicon esculentumMill.) conditions resistance againstPhytophthora infestans

Cited by 135 publications

References 25 publications

Structural, Functional, and Evolutionary Analysis of the Unusually Large Stilbene Synthase Gene Family in Grapevine

Structural, Functional, and Evolutionary Analysis of the Unusually Large Stilbene Synthase Gene Family in Grapevine

A Stress-Inducible Resveratrol O-Methyltransferase Involved in the Biosynthesis of Pterostilbene in Grapevine

Bioassay for the Response of Resveratrol Transgenic Rice Lines to Bacterial and Fungal Diseases

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