Synthesis of a Fluorescent Derivatizing Reagent, 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate, and Its Application for the Analysis of Hydrolysate Amino Acids via High-Performance Liquid Chromatography

Cohen, Steven A.; Michaud, Dennis P.

doi:10.1006/abio.1993.1270

Cited by 906 publications

(526 citation statements)

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“…The concentrations of free amino acids in the spent medium were analysed by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, using a Waters AccQFluor reagent kit (Cohen & Michaud, 1993;Cohen & DeAntonis, 1994). Amino acid derivatives were separated and quantified by reversed-phase (C 18 ) HPLC, using a Waters AccQTag column (15 cm63?9 mm i.d.…”

Section: Methodsmentioning

confidence: 99%

Proteome analysis of Streptococcus mutans metabolic phenotype during acid tolerance

2004

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Two-dimensional gel electrophoretic analysis of the proteome of Streptococcus mutans grown at a steady state in a glucose-limited anaerobic continuous culture revealed a number of proteins that were differentially expressed when the growth pH was lowered from pH 7?0 to pH 5?0. Changes in the expression of metabolic proteins were generally limited to three biochemical pathways: glycolysis, alternative acid production and branched-chain amino acid biosynthesis. The relative level of expression of protein spots representing all of the enzymes associated with the Embden-Meyerhof-Parnas pathway, and all but one of the enzymes involved in the major alternative acid fermentation pathways of S. mutans, was identified and measured. Proteome data, in conjunction with end-product and cell-yield analyses, were consistent with a phenotypic change that allowed S. mutans to proliferate at low pH by expending energy to extrude excess H + from the cell, while minimizing the detrimental effects that result from the uncoupling of carbon flux from catabolism and the consequent imbalance in NADH and pyruvate production. The changes in enzyme levels were consistent with a reduction in the formation of the strongest acid, formic acid, which was a consequence of the diversion of pyruvate to both lactate and branched-chain amino acid production when S. mutans was cultivated in an acidic environment.

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Section: Methodsmentioning

confidence: 99%

Proteome analysis of Streptococcus mutans metabolic phenotype during acid tolerance

2004

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“…Amino acids were analyzed according to the method described by Cohen and Michaud (1993). Flesh fish (5 g) was hydrolyzed in 6 M HCl for 22 h at 110°C and then the identification of amino acids (AAs) was made by Gas-Liquid Chromatography (L1100 Waters) equipped with a quaternary pump, a 20 μl injection valve, a diode array fluorescence detectors and a C18 column (internal diameterof: 250 × 4.6 mm) 5 µm.…”

Section: Amino Acids Analysismentioning

confidence: 99%

Fatty acids and amino acids contents in Scomber scombrus fillets from the South East of Tunisia

Salma¹,

Darej²,

Nizar³

2016

Afr. J. Biotechnol.

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Proximate composition, fats and amino acids of Atlantic Mackerel fillets (Scomber scombrus) from the South East of Tunisia in different seasons, were analyzed in order to assess nutritive characteristics of this species. Samples were collected monthly from Zarzis fishing port located in the South-East of Tunisia. Total fats and protein contents varied significantly (P<0.05) according to the season. The highest values were obtained in spring (11.53 and 24.1% DM, respectively). Gas chromatography analysis showed the existence of saturated (SFAs), monounsaturated (MUFAs) and polyunsaturated (PUFAs) fatty acids. Palmitic and stearic acids were the major fatty acids in SFA family. Palmitoleic and oleic acids were the predominant in MUFA family. Arachidonic acid was the dominant in n-6 PUFA family. While eicosapentanoic (EPA) and docosahexanoic (DHA) were the most abundant in n-3 PUFA series. We observed that high PUFAs percentages were related to those of n-3 PUFA family, mainly DHA which was present at a high level and varied significantly with season (P< 0.01) with the highest value in winter (40%). The n-6 PUFA series were present at low rates comparatively with those of n-3 PUFA series ranging between 4.5 and 5.7%. The highest level of n-6 PUFAs was observed for arachidonic acid in autumn 3.71%. The n-3/n-6 ratio exhibited the highest level in spring (11.02). The Atlantic Mackerel fillets were high in essential amino acids (34.59 g/100 g of proteins). The highest rates were noted for phenylalanine, valine, threonine, isoleucine, leucine and methionine. It was concluded that Atlantic Mackerel was high in interesting human feeding nutriments, mainly PUFA and essential amino Acids. Even when significant, differences between seasons were not drastic and S. scombrus could be consumed during all the year.

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“…HPLC Methods-Glutamate and GABA levels were determined by the Waters AccQ-Tag system utilizing a Waters 2475 fluorescence detector (11). The AccQ-Tag method is based on derivatizing reagent developed specifically for amino acid analysis (6-aminoquinolyl-N-hydroxysuccinimidyl carbamate).…”

Section: Pl00338mentioning

confidence: 99%

Independent Effects of γ-Aminobutyric Acid Transaminase (GABAT) on Metabolic and Sleep Homeostasis

Maguire

Rhoades

Chen

et al. 2015

Journal of Biological Chemistry

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Background: Components of GABA catabolism feed into sleep and potential energy pathways. Results: We identified a metabolic phenotype in Drosophila mutants of GABA turnover and traced it to a limit in glutamate, which is not relevant for sleep. Conclusion: GABA regulates metabolic and sleep homeostasis through independent mechanisms. Significance: Neurological disorders involving GABA disruption may be associated with metabolic problems.

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Synthesis of a Fluorescent Derivatizing Reagent, 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate, and Its Application for the Analysis of Hydrolysate Amino Acids via High-Performance Liquid Chromatography

Cited by 906 publications

References 0 publications

Proteome analysis of Streptococcus mutans metabolic phenotype during acid tolerance

Proteome analysis of Streptococcus mutans metabolic phenotype during acid tolerance

Fatty acids and amino acids contents in Scomber scombrus fillets from the South East of Tunisia

Independent Effects of γ-Aminobutyric Acid Transaminase (GABAT) on Metabolic and Sleep Homeostasis

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