Synthesis of a Biologically Active N-Terminal Tetratriacontapeptide of Parathyroid Hormone

Potts, John T.; Tregear, G. W.; Keutmann, Henry T.; Niall, Hugh D.; Sauer, Robert T.; Deftos, Leonard J.; Dawson, B. F.; Hogan, M. L.; Aurbach, G. D.

doi:10.1073/pnas.68.1.63

Cited by 200 publications

(89 citation statements)

References 14 publications

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“…At 0.08 Amol/min isoproterenol was inactive, but at 0.8 /Amol/ min the iPTH levels increased significantly (P < 0.01) ( (17) suggests that the secretion of PTH is increased. However, because the measurements have not been performed in the venous effluent of the parathyroid glands but in peripheral plasma samples, a metabolic alteration of the circulating iPTH peptides cannot be ruled out.…”

Section: Resultsmentioning

confidence: 94%

Acute Parathyroid Hormone Response to Epinephrine In Vivo

Fischer¹,

Blum²,

Binswanger³

1973

J. Clin. Invest.

153

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A B S T R A C T The acute effects of epinephrine, norepinephrine, and isoproterenol on the plasma immunoreactive parathyroid hormone (iPTH) response were studied in 13 550-600 kg cows. Catecholamines were infused for 7.0 min. During epinephrine infusions at 0.08 Amol/min iPTH increased from 0.48±0.12 (mean+SE, ng/ml) to 1.09±0.18 ng/ml (P < 0.02). Small increases in plasma free fatty acids and glucose could be detected with 0.08 Amol/min epinephrine; the iPTH response to epinephrine was as sensitive as the free fatty acid and glucose responses and possibly of physiological importance. Plasma calcium (total and ionized) and magnesium did not change.The responses were more pronounced at 0.8 /mol/min epinephrine with a mean iPTH increase from 0.49±0.16 ng/ml to 1.74±0.35 ng/ml (P < 0.01). Small decreases in plasma calcium occurred at 0.8 Amol/min epinephrine, but the plasma magnesium remained unchanged. However, when the plasma calcium was lowered with ethylene glycol bis (3-aminoethyl ether) -N, N'-tetraacetic acid (EGTA), a much more pronounced lowering of the plasma calcium was required to produce comparable increases of the plasma iPTH concentrations than when epinephrine was infused. It appears that epinephrine has a direct effect on the release of iPTH from the parathyroid glands.Simultaneous infusions of calcium and epinephrine suppressed the stimulation by epinephrine. This points towards a common mechanism of the regulation of parathyroid hormone secretion caused by decreases in the extracellular calcium concentration and/or alterations in

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Section: Resultsmentioning

confidence: 94%

Acute Parathyroid Hormone Response to Epinephrine In Vivo

Fischer¹,

Blum²,

Binswanger³

1973

J. Clin. Invest.

153

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“…Most of the biological activity of PTH resides in the 1-34 region so that, on a molar basis, PTH(I-34) normally has a potency equal to that of the whole PTH molecule (Potts, Tregear, Keutmann, Niall, Saner, Deftos, Dawson, Hogan and Aurbach, 1971). Similarly, in both the in vivo and in vitro experiments here, the increase in net calcium absorption rate from the rumen associated with the administration of PTH(1-34) was comparable with that found using an equimolar amount of PTHrP(1-34).…”

Section: Discussionmentioning

confidence: 99%

Effects of parathyroid hormone and parathyroid hormone‐related protein on the rates of absorption of magnesium, calcium, sodium, potassium and phosphate ions from the reticulo‐rumen of sheep

Dua

Leonhard²,

Martens³

et al. 1994

Experimental Physiology

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SUMMARYNot only parathyroid hormone (PTH) but also parathyroid hormone-related protein (PTHrP) may play a role in calcium and phosphate homeostasis in ruminants. In five trained sheep, each with a large rumen cannula, the isolated rumen wash technique was used to measure electrolyte absorption rates from the rumen. After two control periods of measurement, 20 pg PTH(1-34) (n = 4), or PTHrP(1-34) (n = 5), was injected intravenously as a loading dose, followed by an infusion of 0-67 pg min-1 over 2 h. Both PTH(l-34) and PTHrP(1-34) significantly increased the absorption rates of calcium and phosphate from the reticulo-rumen. The increases in the absorption rates of magnesium, sodium and potassium observed were not significant. The same technique was also used to demonstrate that increasing the intraruminal calcium concentration from 1 to 4 mmol 1-1 caused corresponding increases in the net rate of absorption of both calcium and inorganic phosphate from the reticulo-rumen. Rumen epithelium was taken from four sheep and mounted in Ussing chambers so that fluxes of calcium could be measured in both directions using 4;Ca. It was found that the addition of 100 ng ml-1 PTH(1-34) or PTHrP( 1-34) to the serosal side increased the net calcium flux rates across the ruminal epithelium. It is concluded that both PTH and PTHrP can influence calcium and phosphate homeostasis in sheep not only by their recognized actions on bone and kidney but also on the absorption of these ions from the forestomachs.

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“…Bovine hormonal fragments 1-34, 14-34, 19-34, and 1-13 were synthesized by a modification of the solid phase methods previously described (9). Fragments 1-34, 14-34, and 19-34 were prepared by removing quantities of peptide-resin at the appropriate steps in a single synthesis beginning with resin-esterified phenylalanine.…”

Section: Methodsmentioning

confidence: 99%