Synthesis of 3β-hydroxy-androsta-5,7-dien-17-one from 3β-hydroxyandrost-5-en-17-one via microbial 7α-hydroxylation

Genotyping of HCV-Egyptian infected cases, and response to treatment

“…Among 482 strains of different taxonomy filamentous fungi tested, mainly Fusarium, Gibberella and Trichothecium representatives catalysed 7␣ -hydroxylation of DHEA (Lobastova et al, 2007;Lobastova et al, 2009), while the strains of Acremonium, Bipolaris, Curvularia expressed 7␤ -hydroxylating activity. Quality control studies has been achieved, as double blind studies were conducted in both Egypt and France .and proved to have 96% correlation, which proves absence of contamination -(No false positive cases).…”

Hydroxylation of 3α- and 3β-hydroxy steroids in position 7 by a fungus of Gibberella zeae VKM F-2600

“…3␣-hydroxy-5␤-cholan-24-oic acid, litocholic acid, LCA). Among 482 strains of different taxonomy filamentous fungi tested, mainly Fusarium, Gibberella and Trichothecium representatives catalysed 7␣ -hydroxylation of DHEA (Lobastova et al, 2007;Lobastova et al, 2009), while the strains of Acremonium, Bipolaris, Curvularia expressed 7␤ -hydroxylating activity.When using LCA as an only substrate, the same strains of Fusarium, Gibberella, Bipolaris and Curvularia genera demonstrated 7␤-hydroxylase activity, thus forming ursodeoxycholic acid (3␣,7␤-dihydroxy-5␤-cholan-24-oic acid, USDA) as a major bioconversion product from LCA. No hydroxylating activity towards LCA was observed for the fungi of Trichothecium and Acremonium tested.…”

“…3␣-hydroxy-5␤-cholan-24-oic acid, litocholic acid, LCA). Among 482 strains of different taxonomy filamentous fungi tested, mainly Fusarium, Gibberella and Trichothecium representatives catalysed 7␣ -hydroxylation of DHEA (Lobastova et al, 2007;Lobastova et al, 2009), while the strains of Acremonium, Bipolaris, Curvularia expressed 7␤ -hydroxylating activity.…”

“…It effectively produced 3␤,7␣-dihydroxyandrost-5-ene-17-one (7␣-OH-DHEA) from DHEA (Lobastova et al, 2009), and expressed high 7␤-hydroxylating activity towards LCA. The conditions of culture growth, target activity induction, and hydroxylation processes performance were optimized.…”

Magnetic capture hybridization technology: a new tool for selective isolation of cell-free circulating DNA from human plasma

“…Among 482 strains of different taxonomy filamentous fungi tested, mainly Fusarium, Gibberella and Trichothecium representatives catalysed 7␣ -hydroxylation of DHEA (Lobastova et al, 2007;Lobastova et al, 2009), while the strains of Acremonium, Bipolaris, Curvularia expressed 7␤ -hydroxylating activity. 3␤-hydroxyandrost-5-ene-17-one, dehydroepiandrosterone, DHEA) were tested for the ability to hydroxylate 3␣-steroid (i.e.…”