Synthesis of 3′-triazoyl-dinucleotides as precursors of stable Phe-tRNAPhe and Leu-tRNALeu analogues

Synthesis of Peptidyl-tRNA Mimics for Structural Biology Applications

“…and co-workers). These include oxadiazole and triazole linkages, − mimicking esters in the 3′ or 2′ position of the 3′-terminal ribose of the aminoacyl-tRNA. The oxadiazole can be introduced into the 3′-position of adenosine, and the corresponding RNA can be obtained following a chemical-enzymatic approach using classical phosphoramidite chemistry to produce the 3′-modified dinucleotides followed by enzymatic ligation using T4 RNA ligase (Figure A).…”