Synthesis of 3-hydroxyretinal in the cytosol of the butterfly compound eye

Shimazaki, Youji; Eguchi, Eisuke

doi:10.1016/0042-6989(93)90154-o

Cited by 6 publications

(10 citation statements)

References 21 publications

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“…On the basis of the results presented here and in our previous paper (Shimazaki and Eguchi 1993), the metabolic pathway postulated for 3-hydroxyretinoids in the eyes of the butterfly is shown in Fig. 8.…”

Section: Discussionmentioning

confidence: 52%

“…3-hydroxyretinoids were extracted separately from the supernatant and the precipitate of the distal and proximal layers alike. The procedure for extracting 3-hydroxyretinoids and the quantitative analyses of 11-cis and all-trans 3-hydroxyretinals were as described previously (Shimazaki and Eguchi 1993). The extracted 3-hydroxyretinoids were analyzed by HPLC-system (Waters Corporation) equipped with a YMC SIL column (6x 150ram).…”

Section: In Vivo Experimentsmentioning

confidence: 99%

“…The peak locations of 3-hydroxyretinoids in the chromatogram were checked by using the standards. Absorption coefficients were used in quantitative estimation of 3-hydroxyretinals (Shimazaki and Eguchi 1993) and all-trans 3-hydroxyretinol (Mayer and Santer 1980). Since the absorption coefficient of 11-cis 3-hydroxyretinol has not been reported, the quantitative estimation of 1 !-cis 3-hydroxyretinol was adjusted on the assumption that the ratio of l l-cis to all-trans isomers of 3-hydroxyretinol is the same as that of retinol (ll-cis retinol, 34,890 at 2 max 318 nm) to all-trans retinol (52,770 at 2 max 325 nm).…”

Section: In Vivo Experimentsmentioning

confidence: 99%

“…However, there is no evidence that all-trans retinol and all-trans 3-hydroxyretinol are related to the synthesis of the ll-cis form of the chromophore. On the other hand, Shimazaki and Eguchi (1993) have reported that, in the eyes of the butterfly Papilio xuthus, an oxidation of 3-hydroxyretinol to 3-hydroxyretinal occurs outside the photoreceptor membrane.…”

Section: Introductionmentioning

confidence: 99%

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Light-dependent metabolic pathway of 3-hydroxyretinoids in the eye of a butterfly, Papilio xuthus

Shimazaki

Eguchi

1995

J Comp Physiol A

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1. After the intact compound eyes of the butterfly Papilio xuthus were adapted to darkness, white, blue (2 max 460 nm) or orange light (2 max 580 nm), the eyes were separated into the distal (primary pigment cells, the dioptric apparatus and ca. 30% of retinal tissue) and the proximal layers (the rest of the retinal tissues). Each layer was separated into a supernatant and a precipitate. Both in white and blue light-adapted eyes, the amount of 11-cis 3-hydroxyretinal increased in the supernatant of the distal layer (Sup-DL) much more than it did in dark-adapted eyes. No increase was observed in the Sup-DL of orange light-adapted eyes.2. When all-trans retinol (non-native chemical) was added to the Sup-DL, it was converted to all-trans retinal under the darkness, and to all-trans and l l-cis retinal by blue light irradiation. When all-trans retinal was added to the Sup-DL, the isomerization of all-trans retinal to 11-cis retinal was accelerated by the blue light.3. The Sup-DL was separated into ammonium sulfate soluble (AS-sup) and insoluble (AS-ppt) fractions. The AS-ppt fraction contained 3-hydroxyretinal but no 3hydroxyretinol. Blue light irradiation to the AS-ppt fraction induced an increase in 11-cis 3-hydroxyretinal, with a concomitant decrease in all-trans 3-hydroxyretinal.These results indicate that both the oxidation of all-trans 3-hydroxyretinol to all-trans 3-hydroxyretinal and the light-dependent isomerization of all-trans 3hydroxyretinal to 11-cis isomer take place in the tissues of the distal layer of the eyes.

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Section: Discussionmentioning

confidence: 52%

Section: In Vivo Experimentsmentioning

confidence: 99%

Section: In Vivo Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Light-dependent metabolic pathway of 3-hydroxyretinoids in the eye of a butterfly, Papilio xuthus

Shimazaki

Eguchi

1995

J Comp Physiol A

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“…Of course, so far we have no direct evidence to indicate that the primary pigment cells are the site where the visual cycle is taking place in Papilio. However, the distal portion of the Papilio retina functions in the light-dependent oxidation of 3-hydroxyretinol and isomerization of 3-hydroxyretinal (Shimazaki and Eguchi, 1993). We have also demonstrated that Papilio RBP is involved in retinoid metabolism in the distal portion of the eye (Wakakuwa et al, 2003).…”

Section: Discussionmentioning

confidence: 69%

Immunohistochemical localization of Papilio RBP in the eye of butterflies

Wakakuwa

Ozaki

Arikawa

2004

Journal of Experimental Biology

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SUMMARY We recently identified a novel retinoid binding protein, PapilioRBP, in the soluble fraction of the eye homogenate of the butterfly Papilio xuthus, and demonstrated that the protein is involved in the visual cycle. We now have localized the protein in the Papilio eye by light and electron microscopic immunohistochemistry using a monospecific antiserum produced against artificially expressed Papilio RBP. We found strong immunoreactivity in the primary as well as secondary pigment cells and in the tracheal cells. The pigment cells have long been regarded as an important site of the visual cycle, and this view is further supported by the present result. Interestingly, the cytoplasm and nuclei of these cells were equally labeled, indicating that the protein exists in both the cytoplasm and the nucleus. We conducted a survey for the existence of the Papilio RBP-like proteins in other insects including several species of butterflies, dragonflies, cicadas, grasshoppers and honeybees. Anti-Papilio RBP immunoreactivity was confirmed in the proteins isolated only from butterflies belonging to the superfamily Papilionoidea and not from other species. In all insects tested, however, fluorescing proteins were clearly detected, suggesting that these insects also have similar retinol-binding proteins.

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Light-dependent metabolic pathway of 3-hydroxyretinoids in the eye of a butterfly,Papilio xuthus

Shimazaki¹,

Eguchi²

1995

J Comp Physiol A

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Synthesis of 3-hydroxyretinal in the cytosol of the butterfly compound eye

Cited by 6 publications

References 21 publications

Light-dependent metabolic pathway of 3-hydroxyretinoids in the eye of a butterfly, Papilio xuthus

Light-dependent metabolic pathway of 3-hydroxyretinoids in the eye of a butterfly, Papilio xuthus

Immunohistochemical localization of Papilio RBP in the eye of butterflies

Light-dependent metabolic pathway of 3-hydroxyretinoids in the eye of a butterfly,Papilio xuthus

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