Synthesis of 2-phenylethyl acetate in the presence of Yarrowia lipolytica KKP 379 biomass

Białecka‐Florjańczyk, Ewa; Krzyczkowska, Jolanta; Stolarzewicz, I.; Kapturowska, A. U.

doi:10.1016/j.molcatb.2011.10.010

Cited by 33 publications

(20 citation statements)

References 33 publications

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“…Yarrowia lipolytica can also be used for the production of aroma chemicals (Bialecka-Florjanczyk et al, 2012;Garcia et al, 2009) and, in particular, of g-decalactone, which has a characteristic peach flavor. g-Decalactone is typically produced using methyl ricinoleate derived from castor oil as substrate (Gomes et al, 2012;Guo et al, 2012;Rabenhorst & Gatfield, 2002;Schrader et al, 2004).…”

Section: Industrial Uses Of Y Lipolytica: Past Present and Futurementioning

confidence: 99%

Yarrowia lipolytica: Safety assessment of an oleaginous yeast with a great industrial potential

Groenewald

Boekhout

Neuvéglise

et al. 2013

Critical Reviews in Microbiology

384

288

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Yarrowia lipolytica has been developed as a production host for a large variety of biotechnological applications. Efficacy and safety studies have demonstrated the safe use of Yarrowia-derived products containing significant proportions of Yarrowia biomass (as for DuPont's eicosapentaenoic acid-rich oil) or with the yeast itself as the final product (as for British Petroleum's single-cell protein product). The natural occurrence of the species in food, particularly cheese, other dairy products and meat, is a further argument supporting its safety. The species causes rare opportunistic infections in severely immunocompromised or otherwise seriously ill people with other underlying diseases or conditions. The infections can be treated effectively by the use of regular antifungal drugs, and in some cases even disappeared spontaneously. Based on our assessment, we conclude that Y. lipolytica is a ''safe-to-use'' organism.

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Section: Industrial Uses Of Y Lipolytica: Past Present and Futurementioning

confidence: 99%

Yarrowia lipolytica: Safety assessment of an oleaginous yeast with a great industrial potential

Groenewald

Boekhout

Neuvéglise

et al. 2013

Critical Reviews in Microbiology

384

288

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“…, and the ability of some yeasts to act as a catalyst in the transesterification of 2-PE and ethyl acetate (other product presented in significant amounts in the medium) into 2-PEA due to their lipolytic activity have been studied (Białecka-Florjańczyk et al 2012). Although K. marxianus is not typically lipase active, prior investigations (Deive et al 2003) have proven the capability of this strain to produce extracellular lipolytic activity.…”

Section: )mentioning

confidence: 99%

Bioproduction of 2-phenylethanol and 2-phenethyl acetate by Kluyveromyces marxianus through the solid-state fermentation of sugarcane bagasse

Martínez

Sánchez

Font

et al. 2018

Appl Microbiol Biotechnol

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2-Phenylethanol (2-PE) and 2-phenethyl acetate (2-PEA) are important aroma compounds widely used in food and cosmetic industries due to their rose-like odor. Nowadays, due to the growing demand for natural products, the development of bioprocesses for obtaining value-added compounds has become of great significance. 2-PE and 2-PEA can be produced through the biotransformation of L-phenylalanine using the generally recognized as safe strain Kluyveromyces marxianus. L-phenylalanine bioconversion systems have been typically focused on submerged fermentation processes (SmF), but there is no information about other alternative productive approaches. Here, the solid-state fermentation (SSF) of sugarcane bagasse supplemented with L-phenylalanine was investigated as a sustainable alternative for producing 2-PE and 2-PEA in a residue-based system using Kluyveromyces marxianus as inoculum. An initial screening of the operational variables indicated that air supply, temperature, and initial moisture content significantly affect the product yield. Besides, it was found that the feeding strategy also affects the production and the efficiency of the process. While a basic batch system produced 16 mg per gram of residue (dry basis), by using split feeding strategies (fed-batch) of only sugarcane bagasse, a maximum of 18.4 mg g were achieved. Increase in product yield was also accompanied by an increase in the consumption efficiency of nutrients and precursor. The suggested system results as effective as other more complex SmF systems to obtain 2-PE and 2-PEA, showing the feasibility of SSF as an alternative for producing these compounds through the valorization of an agro-industrial residue.

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“…For the optimization purposes four different mediums previously used for the production of 2phenylethyl alcohol by yeast were evaluated. Media compositions were selected on the basis of published papers by Białecka-Florjańczyk [1], Etschmann [4], Mei [7] and Wang [9] with small modifications. Preparing medium 1 bacto-peptone was changed into soy peptone and 3 g/l maltose extract was added.…”

Section: Resultsmentioning

confidence: 99%

“…For this reason possibly the most natural products, sold at affordable prices are in greatest demand. In the case of production of aroma compounds an alternative is provided by the application of methods based on biotechnology [1]. Although these processes are connected with the need for adequate selection and optimization of strains capable of production aroma compounds at the genotype and phenotype levels, a process competitive in relation to conventional technologies may be developed thanks to the appropriate selection of medium composition and physical parameters of culture.…”

Section: Introductionmentioning

confidence: 99%

Analysis of the ability to form 2-phenylethyl alcohol by Galactomyces geotrichum MK017

Grygier

Majcher

Myszka

2015

zntj

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The aim of the study was to evaluate the ability of Galactomyces geotrichum MK017 for the biosynthesis of 2-phenylethyl alcohol and optimization of the culture medium composition in order to increase the yield of the product. The culture of Galactomyces geotrichum MK017 strain was carried out in the laboratory scale. For isolation of volatiles Solid Phase Microextraction (SPME) has been used. The identification and quantification of aroma compounds produced by examined microflora was determined by gas chromatography and mass spectrometry (GC/MS) system. The results showed that the tested mould Galactomyces geotrichum MK017 shows the potential for the production of 2-phenylethyl alcohol, but also for the synthesis of phenylacetaldehyde, phenylacetic and phenyllactic acids. For the optimization of the 2phenylethyl alcohol production yield four different medium composition have been tested. The bioprocess of aroma compound production by tested microorganism was the most efficient on the medium composed of sucrose (80 g/l) and L-phenylalanine (21 g/l) and pH value of 5.0. Using this composition in a batch culture of 770 ml volume the highest concentration of 2-phenylethyl alcohol has been obtained-6 mg/l. At the same time amount of phenylacetaldehyde, phenylacetic acid and phenyllactic acidhas reached 2.2 mg/l, 10.66 mg/l and 32.3 mg/l respectively.

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Synthesis of 2-phenylethyl acetate in the presence of Yarrowia lipolytica KKP 379 biomass

Cited by 33 publications

References 33 publications

Yarrowia lipolytica: Safety assessment of an oleaginous yeast with a great industrial potential

Yarrowia lipolytica: Safety assessment of an oleaginous yeast with a great industrial potential

Bioproduction of 2-phenylethanol and 2-phenethyl acetate by Kluyveromyces marxianus through the solid-state fermentation of sugarcane bagasse

Analysis of the ability to form 2-phenylethyl alcohol by Galactomyces geotrichum MK017

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