Synthesis of 17,20α/β-Dihydroxy-4-pregnen-3-one and 5β-Pregnanes in Spermatozoa of Primary and 17α-Methyltestosterone-Induced Secondary Male Grouper (Epinephelus coioides)

Lee, S.T.L.; Kime, David E.; Lam, T.J.; Tan, Cheong Huat

doi:10.1006/gcen.1998.7122

Cited by 18 publications

(5 citation statements)

References 22 publications

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“…In other species, spermatozoa incubated with 17‐P produce either mainly 17,20α‐P including: P. platessa (Canario & Scott, 1990), greasy grouper Epinephelus tauvina (Forsskål) (Tan et al , 1995), C. carpio (Asahina et al , 1990; Barry et al , 1990 b ) and C. auratus (Asahina et al , 1993; Ebrahimi et al , 1996). There are other species in which the spermatozoa convert 17‐P to a range of steroids including: roughly equal amounts of 17,20α‐P and 17,20β‐P in flounder Platichthys flesus (L.) (Asahina et al , 1994), a range of 5β‐pregnanes, 17,20α‐P and a trace of 17,20β‐P in male E. coioides (Lee et al , 1998), and a mixture of 17,20β‐P, 17,20α‐P, 11‐KT and androstenedione in R. rutilus , with, however, exclusive production of 17,20α‐P in the presence of excess 17‐P (Ebrahimi et al , 1995).…”

Section: Evidence For the Two‐cell Origin Of 1720β‐p In The Testismentioning

confidence: 99%

The role of the maturation‐inducing steroid, 17,20β‐dihydroxypregn‐4‐en‐3‐one, in male fishes: a review

Scott

Sumpter

Stacey

2010

Journal of Fish Biology

147

119

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The major progestin in teleosts is not progesterone, as in tetrapods, but 17,20beta-dihydroxypregn-4-en-3-one (17,20beta-P) or, in certain species, 17,20beta,21-trihydroxy-pregn-4-en-3-one (17,20beta,21-P). Several functions for 17,20beta-P and 17,20beta,21-P have been proposed (and in some cases proved). These include induction of oocyte final maturation and spermiation (milt production), enhancement of sperm motility (by alteration of the pH and fluidity of the seminal fluid) and acting as a pheromone in male cyprinids. Another important function, initiation of meiosis (the first step in both spermatogenesis and oogenesis), has only very recently been proposed. This is a process that takes place at puberty in all fishes and once a year in repeat spawners. The present review critically examines the evidence to support the proposed functions of 17,20beta-P in males, including listing of the evidence for the presence of 17,20beta-P in the blood plasma of male fishes and discussion of why, in many species, it appears to be absent (or present at low and, in some cases, unvarying concentrations); consideration of the evidence, obtained mainly from in vitro studies, for this steroid being predominantly produced by the testis, for its production being under the control of luteinizing hormone (gonadotrophin II) and, at least in salmonids, for two cell types (Leydig cells and sperm cells) being involved in its synthesis; discussion of the factors involved in the regulation of the switch from androgen to 17,20beta-P production that seems to occur in many species just at the time of spermiation; discussion of the effects of in vivo injection and application of 17,20beta-P (and closely related compounds) in males; a listing of previously published evidence that supports the proposed new function of 17,20beta-P as an initiator of meiosis; finally, discussion of the evidence for environmental endocrine disruption by progestins in fishes.

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Section: Evidence For the Two‐cell Origin Of 1720β‐p In The Testismentioning

confidence: 99%

The role of the maturation‐inducing steroid, 17,20β‐dihydroxypregn‐4‐en‐3‐one, in male fishes: a review

Scott

Sumpter

Stacey

2010

Journal of Fish Biology

147

119

View full text Add to dashboard Cite

show abstract

“…Spermatogenesis in teleosts, as in other vertebrates, involves a complex interaction of various hormones along the brain-pituitary-gonad axis (Degani et al , 1998;Lee et al ., 1998). One of these hormones is gonadotropin (GtH), a glycoprotein consisting of two subunits, α and β , which are non-covalently bound.…”

Section: Introductionmentioning

confidence: 99%

βFSH, βLH and Growth Hormone Gene Expression in Blue Gourami (Trichogaster trichopterus, Pallas 1770) During Spermatogenesis and Male Sexual Behavior

et al. 2003

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The relationship between gonadal development (histological evidence for spermiogenesis and/or spermatogenesis), sexual behavior (nest-building) and mRNA levels of gonadotropins (betaFSH and betaLH) and growth hormone (GH) in the male pituitary was investigated. Amplification of betaFSH cDNA showed a significantly higher mRNA level in mature males (whether sexually active or not) than in juveniles. However, following PCR amplification of betaLH cDNA, a significantly higher mRNA level was found in the sexually active group compared to the sexually inactive group. These results suggest that FSH may participate in spermatogenesis, whereas LH is more involved in spermiogenesis. The GH mRNA level increased slightly during the maturation process but no significant differences were found between the groups studied.

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“…Therefore, male groupers have become the limiting factor for mass production of grouper fry and large-scale aquaculture of the commercial fish. Sex inversion has been successfully induced by the implantation of 17 α-methyltestosterone, but there is poor spermiation in the precocious secondary males (Lee et al, 1998). Previous studies on grouper reproductive biology were mainly focused on the gonad ultrastructure and histology at different developmental stages (Lee et al, 1998;Yeh et al, 2003b), but little molecular information was known with regard to the sex inversion.…”

Section: Introductionmentioning

confidence: 99%

EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides)

et al. 2006

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Synthesis of 17,20α/β-Dihydroxy-4-pregnen-3-one and 5β-Pregnanes in Spermatozoa of Primary and 17α-Methyltestosterone-Induced Secondary Male Grouper (Epinephelus coioides)

Cited by 18 publications

References 22 publications

The role of the maturation‐inducing steroid, 17,20β‐dihydroxypregn‐4‐en‐3‐one, in male fishes: a review

The role of the maturation‐inducing steroid, 17,20β‐dihydroxypregn‐4‐en‐3‐one, in male fishes: a review

βFSH, βLH and Growth Hormone Gene Expression in Blue Gourami (Trichogaster trichopterus, Pallas 1770) During Spermatogenesis and Male Sexual Behavior

EST-based identification of genes expressed in the hypothalamus of male orange-spotted grouper (Epinephelus coioides)

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