Synthesis of (1→2)-β-d-Glucan by Cell-free Extracts ofAgrobacterium radiobacterIFO 12665b 1 andRhizobium phaseoliAHU 1133

Synthetic Cyclic Oligosaccharides

“…The biosynthesis of β-(1→2)-glucopyranooligosaccharides has been studied extensively and in vitro preparations 128 have been performed. Isolation of the individual compounds is only possible by HPLC . However, the fact that the cyclic β-(1→2)-glucans possess 133 some biological activity has stimulated investigations on their structures, mostly by NMR spectroscopy 134 and by molecular modeling …”

The Rhizobiaceae

“…Castro et al (Castro et al, 1995) have provided evidence that the NdvB protein of R. leguminosarum is slightly larger than the NdvB protein of S. meliloti. The structures of the neutral cyclic P-(1,2)glucans produced in vitro are indistinguishable from those produced in vivo (Amemura 1984;Breedveld et al, 1992;Williamson et al, 1992). This intermediate has been detected in in vitro analyses of cyclic P-(l,2)-glucan biosynthesis from UDP-glucose using membrane preparations derived from Rhizobium and Agrobaeterium (Geremia et al, 1987;Zorreguieta and Ugalde 1986;.…”

“…This intermediate has been detected in in vitro analyses of cyclic P-(l,2)-glucan biosynthesis from UDP-glucose using membrane preparations derived from Rhizobium and Agrobaeterium (Geremia et al, 1987;Zorreguieta and Ugalde 1986;. sophorose) or other primers are needed for the synthesis of the glucan ring (Amemura 1984;Breedveld et al, 1992;Williamson et al, 1992;Zorreguieta et aI., 1985). In an attempt to further purify the cyclic P-(l,2)-glucan synthetase activity from membrane preparations of A.…”

“…The structures of the neutral cyclic P-(1,2)glucans produced in vitro are indistinguishable from those produced in vivo (Amemura 1984;Breedveld et al, 1992;Williamson et al, 1992). (ii) Only cyclic forms of the P-(l,2)-glucans are detected after release from these proteins (Amemura 1984;Breedveld et al, 1992;Williamson et al, 1992;. radiobaeter, Kinoshita and coworkers (Kinoshita et al, 1991) identified 2 activities on the basis of binding behavior on an anion exchange column.…”

pH-Dependent Inclusion Complexation of Highly Succinylated Cyclosophoraoses with 4'-Hydroxyflavanone

“…2, 3 They have also been reported to be involved in nitrogen fixation, 4 attachment of bacterial cells to plant hosts 5 and osmotic regulation. 6 They have attracted considerable attention because of their potential ability to form inclusion complexes with other hydrophobic molecules as well as on their various biological functions.…”