Synthesis, DNA binding and photocleavage, and cellular uptake of an alkyl chain-linked dinuclear ruthenium(II) complex

Liu, Ping; Jin, Ling; Zhang, Yuqi; Wu, Baoyan; Wang, Kezhi

doi:10.1016/j.jphotobiol.2015.01.004

Cited by 30 publications

(4 citation statements)

References 60 publications

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“… These complexes exhibit rapid cellular uptake in live HeLa cells, primarily residing in the cytoplasm with negligible effect on the cell viability. Similar cytoplasmic localization has also been observed in other binuclear ruthenium(II) complexes ( 28 and 29 ). , Lincoln and co-workers have studied the subcellular distribution of the stereoisomers of two binuclear ruthenium(II) complexes ( 30 ) in fixed CHO-K1 cells and found that while both the ΔΔ- and ΛΛ-isomers of the complexes show prominent nuclear staining, the ΛΛ-isomers exclusively accumulate in the nucleoli . However, no significant difference is observed in the cellular uptake and localization of the complexes in live cells, where they are entrapped in the endosomes after endocytic uptake.…”

Section: Luminescent Transition Metal Complexes For Bioimagingsupporting

confidence: 57%

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Lee,

2024

Chem. Rev.

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Luminescence imaging is a powerful and versatile technique for investigating cell physiology and pathology in living systems, making significant contributions to life science research and clinical diagnosis. In recent years, luminescent transition metal complexes have gained significant attention for diagnostic and therapeutic applications due to their unique photophysical and photochemical properties. In this Review, we provide a comprehensive overview of the recent development of luminescent transition metal complexes for bioimaging and biosensing applications, with a focus on transition metal centers with a d 6 , d 8 , and d 10 electronic configuration. We elucidate the structure−property relationships of luminescent transition metal complexes, exploring how their structural characteristics can be manipulated to control their biological behavior such as cellular uptake, localization, biocompatibility, pharmacokinetics, and biodistribution. Furthermore, we introduce the various design strategies that leverage the interesting photophysical properties of luminescent transition metal complexes for a wide variety of biological applications, including autofluorescence-free imaging, multimodal imaging, organelle imaging, biological sensing, microenvironment monitoring, bioorthogonal labeling, bacterial imaging, and cell viability assessment. Finally, we provide insights into the challenges and perspectives of luminescent transition metal complexes for bioimaging and biosensing applications, as well as their use in disease diagnosis and treatment evaluation.

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Section: Luminescent Transition Metal Complexes For Bioimagingsupporting

confidence: 57%

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Lee,

2024

Chem. Rev.

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“…The metal-based PS [Ru(bpy) 3 ] 2+ is expected to be more stable under the catalytic conditions than EY. However, in the absence of an RM, no significant hydrogen evolution could be observed presumably due to the inability of [Ru(bpy) 3 ] 2+ to permeate the cell membrane . Conversely, EY is a known cytoplasmic staining agent .…”

Section: Resultsmentioning

confidence: 99%

Elucidating Electron Transfer Kinetics and Optimizing System Performance for Escherichia coli-Based Semi-Artificial H₂ Production

et al. 2023

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Both photo-and biocatalysis are well-established and intensively studied. The combination of these two approaches is also an emerging research field, commonly referred to as semi-artificial photosynthesis. Semi-artificial photosynthesis aims at combining highly efficient synthetic light harvesters with the self-healing and potent catalytic properties of biocatalysis. In this study, a semi-artificial photocatalytic system featuring Escherichia coli bacteria, which heterologously express the [FeFe] hydrogenase enzyme HydA1 from green algae, is employed as a hydrogen gas production catalyst. To probe the influence of photochemistry on overall system performance, the E. coli whole-cell catalyst is combined with two different photosensitizers and redox mediators. The addition of a redox mediator greatly improves the rates and longevity of the photocatalytic system, as reflected in increases of both the turn-over number (0.777 vs 10.9 μmol H 2 mL −1 OD 600 −1) and the turn-over frequency (175 vs 334 μmol H 2 mL −1 h −1 OD 600 −1). The redox mediator is found to both protect from photobleaching and enable electron transport to the hydrogenase from an extracellular photosensitizer. However, E. coli cells are strongly affected by the photocatalytic system, leading to a decrease in cell integrity and cell viability, possibly due to toxic decomposition products formed during the photocatalytic process. We furthermore employed steady-state and transient absorption spectroscopy to investigate solution potentials and the kinetics of electron transfer processes between the sacrificial electron donor, photosensitizer, redox mediator, and the [FeFe] hydrogenase as the final electron acceptor. These results allowed us to rationalize the different activities observed in photocatalytic assays and offer a better understanding of the factors that influence the photocatalytic performance of E. coli-based whole-cell systems.

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“…At low concentrations of DNA (60 to 360 µM), no obvious changes in the spectrum are observed. While, upon increasing concentrations of DNA (420 to 660 µM), a hypochromism (as calculated by [Afree− Abound/ Afree]×100) at ∼ 282 nm reaches as high as 14.0% at fnal DNA concentration and a bathochromic value of 4.0 nm (as calculated by Δλ=λbound−λfree) were seen (Liu et al 2015). These evident spectral changes indicate that the complex interacts with DNA via intercalation (Anjomshoa et al 2019).…”

Section: Dna-binding Afnity and Modementioning

confidence: 98%

In vitro biological and in silico molecular docking and ADME studies of a substituted triazine-coordinated cadmium(II) ion: efficient cytotoxicity, apoptosis, genotoxicity, and nuclease-like activity plus binding affinity towards apoptosis-related proteins

et al. 2022

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Synthesis, DNA binding and photocleavage, and cellular uptake of an alkyl chain-linked dinuclear ruthenium(II) complex

Cited by 30 publications

References 60 publications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Elucidating Electron Transfer Kinetics and Optimizing System Performance for Escherichia coli-Based Semi-Artificial H₂ Production

In vitro biological and in silico molecular docking and ADME studies of a substituted triazine-coordinated cadmium(II) ion: efficient cytotoxicity, apoptosis, genotoxicity, and nuclease-like activity plus binding affinity towards apoptosis-related proteins

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Synthesis, DNA binding and photocleavage, and cellular uptake of an alkyl chain-linked dinuclear ruthenium(II) complex

Cited by 30 publications

References 60 publications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Elucidating Electron Transfer Kinetics and Optimizing System Performance for Escherichia coli-Based Semi-Artificial H2 Production

In vitro biological and in silico molecular docking and ADME studies of a substituted triazine-coordinated cadmium(II) ion: efficient cytotoxicity, apoptosis, genotoxicity, and nuclease-like activity plus binding affinity towards apoptosis-related proteins

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Elucidating Electron Transfer Kinetics and Optimizing System Performance for Escherichia coli-Based Semi-Artificial H₂ Production