Synthesis, Characterization, Spectrofluorometric and Antibacterial Activity Studies of Moxifloxacin- Zirconium Complex

Abstract

PECTROSCOPIC methods such as mass, FT-IR and nuclear ........magnetic resonance in combination with thermal analysis measurements were used to verify and describe the physicochemical properties of the synthesized moxifloxacin (MOX) Zirconium (IV) metal complex. The spectroscopic and elemental analysis data support the formation of the complex with the formula C 21 H 23 FN 3 O 4 Zr(H 2 O) 2 .0.5H 2 O. Results revealed that complexation between Zirconium (IV) and moxifloxacin exhibited significant increase in antibacterial activity especially against Gram negative organisms.In addition, a simple, rapid, reliable, and sensitive spectrofluorometric method is developed for the determination of MOX. The method depends on the chelation of MOX with zirconium (IV) to produce fluorescent chelate (MOX/ Zr). Different factors affecting the relative fluorescence intensity of the resulting chelate were studied and optimized. The relationship between the concentration and relative fluorescence intensity was rectilinear in the range of 0.1-4 µg/ml. The limits of detection and quantitation are 0.06 and 0.11 µg/ ml, respectively.At the optimum reaction conditions, the drug-metal chelate showed excitation maximum at 333 nm and emission maxima at 485nm. The developed method was applied successfully for the determination of the studied drug in its pharmaceutical dosage forms with a good precision and accuracy.