Synthesis, characterization, HSA/DNA interactions and antitumor activity of new [Ru(η6-p-cymene)Cl2(L)] complexes

Abstract

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Mo(VI) Potential Metallodrugs: Explaining the Transport and Cytotoxicity by Chemical Transformations

“…The cell suspension was gently mixed and incubated for 30 min at 37 °C in the dark. Cell cycle was analyzed using a BD Accuri C6 flow cytometer …”

“…Cell cycle was analyzed using a BD Accuri C6 flow cytometer. 92 Cell Apoptosis Assay. Analysis of apoptotic populations of HeLa cells induced by the complexes 1−4 were determined by Annexin V and PI staining according to the manufacturer's protocol for the Annexin V:FITC Apoptosis Detection Kit (Abgenex) by flow cytometry.…”

Integrated experimental/computational approaches to characterize the systems formed by vanadium with proteins and enzymes

“…Recent applications are the binding elucidation of Pt-and Ru-based metallodrugs in proteins as well as the Cu-and Mn-based cofactors in artificial metalloenzymes. 9,[174][175][176] In the field of metallodrug design, the binding sites of antiarthritic Auranofin and other cytotoxic gold compounds under experimentation share the same structural motif and the [Au I (PEt 3 )] + moiety is released in aqueous solution. [177][178][179] HSA is the protein candidate for the transport of Au(I) species in humans, even though no X-ray determinations are reported for the adducts between the Au I (PEt 3 ) + moiety and HSA.…”

“…174 Similar integration between coordination dockings, MDs and spectroscopy have been recently applied for the study of the interaction of [Ru II (η 6 -p-cymene)Cl 2 (L)] potential metallodrugs, where L is an isothiazole ligand, to calf thymus deoxyribonucleic acid (CT-DNA) and HSA. 175 The modelling ascertained the coordination of Ru II to N(7)dG7 of CT-DNA and Glu292 of HSA; notably, the latter remembers the binding of [Ru II (1,4,7-trithiacyclononane)(ethylenediamine)Cl] + to lysozyme through the side-chain of Asp101 and to proteinase K through Asp200 or Asp260, which replace the released chloride ligand. 187 The results supported the affinity of these potential drugs either toward the CT-DNA major groove or to the IIa subdomain of HSA.…”

Probing the toxic interactions between the reactive dye Drimaren Red and Human Serum Albumin

“…The UV-Vis technique is an excellent tool to verify the formation of a protein-ligand complex in the fundamental state and changes in the polypeptidic structure of the protein (Bratty, 2020;Santos et al, 2018). The formation of this complex is confirmed by changes in the characteristic wavelengths of the UV-Vis spectrum of the protein when it comes into contact with the test substance, such as changes in the absorption intensity and peak displacements (Ahmad et al, 2020;Đ ukić et al, 2020). Fig.…”