A novel ternary copper (II) complex have been synthesized by the addition of N, N-heterocyclic ligand with L-amino acid. The copper (II) complex, [Cu(1,10-phen) (L-Thr) Br] (where phen=1,10-phenanthroline and Threo=Threonine) characterized by various spectroscopic method. The intense UV band around 271nm was due to π-π* transition. The DNA binding study of these copper (II) complex are examined by UV–Visible, Emission spectroscopic, cyclic voltammetric and viscosity method. The results revealed that complex shown to be a intercalation mode of binding into DNA. The anticancer activity of Cu (II) complexes has capability to the kill HepG2 liver cancer cell as assessed by the MTT method, The Ic50value was found 21.50µg/ml The biological activity of the complex tested against certain pathogenic bacteria and fungi results revealed it was found to be potent antibacterial agent.