Synthesis, Characterization, Antibacterial, DNA Binding and Cleavage Studies of Mixed Ligand Cu(II), Co(II) Complexes

Ushaiah, B.; leela, D. Shiva; Ravi, Malini; Anupama, B.; Shyam, Perugu; Kumari, C. Gyana

doi:10.1007/s10895-014-1456-2

Cited by 10 publications

(4 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mean values of three replicated measurements were used to evaluate the viscosity η of the samples. Data are presented as ( η / η 0 ) 1/2 versus binding ratio of [compound]/[DNA], where η and η 0 are the viscosity of DNA in the absence and presence of compounds.…”

Section: Methodsmentioning

confidence: 99%

Synthesis, structural characterization, antimicrobial, antioxidant and DNA binding studies of some novel homo‐binuclear Schiff base metal (II) complexes

Aziz

Shawky

Khalil

2018

Applied Organom Chemis

View full text Add to dashboard Cite

A novel bi‐nucleating Schiff base ligand, 6,6′‐(((1E,1′E)‐thiophene‐2,5‐diylbis (methaneylylidene))bis (azaneylylidene))bis (3,4‐dimethylaniline), and five binuclear M (II) complexes were synthesized. The bi‐nucleating Schiff base ligand and its metal complexes were characterized using various physicochemical techniques, e.g. elemental analyses, spectroscopic methods, conductivity and magnetic moment measurements. The low molar conductance of the complexes in dimethylsulfoxide shows their non‐electrolytic nature. The antibacterial activities were screened against pathogenic bacteria (Staphylococcus aureus, Escherichia coli, Pseudomonas putida and Bacillus subtilis). The antifungal activity was screened against Aspergillus niger, Aspergillus flavus and Rhizoctonia bataicola. The antimicrobial activity data showed that the metal complexes are more potent than the parent Schiff base ligand against microorganisms. The antioxidant activities of the synthesized compounds were investigated through scavenging activity against 2,2‐diphenyl‐2‐picrylhydrazyl, superoxide anion, hydroxyl and 2,2′‐azinobis (3‐ethylbenzothiazoline‐6‐sulfonic acid) radicals. The complexes have superior radical scavenging activity than the free ligand and the scavenging effects of the Cu (II) complex are stronger than those of the other complexes. DNA binding studies were performed using electronic spectroscopy, fluorometric competition studies and viscosity measurements. The data indicated that there is a marked enhancement in biocidal activity of the ligand under similar experimental conditions because of coordination with metal ions.

show abstract

Section: Methodsmentioning

confidence: 99%

Synthesis, structural characterization, antimicrobial, antioxidant and DNA binding studies of some novel homo‐binuclear Schiff base metal (II) complexes

Aziz

Shawky

Khalil

2018

Applied Organom Chemis

View full text Add to dashboard Cite

show abstract

“…Spectrophotometric technique is employed in the following experiments, viz; (i) for the determination of the composition of metal complex by Job's continuous variation and Mole ratio methods, (ii) for the determination of order of reaction in the formation of metal complex and thermodynamic parameters such as activation energy (E a ), entropy change (ΔS * ), free energy change (ΔG * ) and enthalpy change (ΔH * ) [36], (iii) for determination of antioxidant properties using 2, 2diphenyl-picrylhydrazyl (DPPH) by in-vitro assay method [37], (iv) Binding ability of metal complexes with CT-DNA.…”

Section: Spectrophotometric Studiesmentioning

confidence: 99%

“…Flow time was measured with a digital stop watch; three times for each sample and an average flow time was calculated. Data were presented as (η/η 0 ) 1/3 versus binding ratio [37].…”

Section: Viscosity Measurementmentioning

confidence: 99%

See 1 more Smart Citation

A Study on Spectro-Analytical Aspects, DNA – Interaction, Photo-Cleavage, Radical Scavenging, Cytotoxic Activities, Antibacterial and Docking Properties of 3 – (1 – (6 – methoxybenzo [d] thiazol – 2 – ylimino) ethyl) – 6 – methyl – 3H – pyran - 2, 4 – dione and its Metal Complexes

et al. 2015

Self Cite

View full text Add to dashboard Cite

The focus of the present work is on the design, synthesis, characterization, DNA-interaction, photo-cleavage, radical scavenging, in-vitro cytotoxicity, antimicrobial, docking and kinetic studies of Cu (II), Cd (II), Ce (IV) and Zr (IV) metal complexes of an imine derivative, 3 - (1 - (6 - methoxybenzo [d] thiazol - 2 - ylimino) ethyl) - 6 - methyl - 3H - pyran - 2, 4 - dione. The investigation of metal ligand interactions for the determination of composition of metal complexes, corresponding kinetic studies and antioxidant activity in solution was carried out by spectrophotometric methods. The synthesized metal complexes were characterized by EDX analysis, Mass, IR, (1)H-NMR, (13)C-NMR and UV-Visible spectra. DNA binding studies of metal complexes with Calf thymus (CT) DNA were carried out at room temperature by employing UV-Vis electron absorption, fluorescence emission and viscosity measurement techniques. The results revealed that these complexes interact with DNA through intercalation. The results of in vitro antibacterial studies showed the enhanced activity of chelating agent in metal chelated form and thus inferring scope for further development of new therapeutic drugs. Cell viability experiments indicated that all complexes showed significant dose dependent cytotoxicity in selected cell lines. The molecular modeling and docking studies were carried out with energy minimized structures of metal complexes to identify the receptor to metal interactions.

show abstract

DNA binding, DNA cleavage and HSA interaction of several metal complexes containing N-(2-hydroxyethyl)-N′-benzoylthiourea and 1,10-phenanthroline ligands

Peng

Gao

et al. 2016

J Biol Inorg Chem

View full text Add to dashboard Cite

Four novel ternary metal complexes of the type [M(Phen)(L1)2)] [phen = 1,10-phenanthroline, L1 = N-(2-hydroxyethyl)-N'-benzoylthiourea, M = Ni(II)(1), Co(II) (2), Cu(II) (3), Pd(II) (4)] were synthesized. The organic ligands and their corresponding organometallic complexes have been characterized using UV-vis absorption spectroscopy, element analysis, infrared radiation spectroscopy and fluorescence spectra. DNA binding and cleavage studies of these complexes were conducted in detail. In vitro DNA-binding properties were studied by electronic absorption spectra and fluorescence spectra methods. The results indicate that all of the ternary metal complexes can efficiently bind to DNA via intercalation mode. The DNA-binding constants for all ternary compounds are around 4 × 10(6) M(-1). The binding propensity of the complexes to human serum albumin (HSA) was also investigated. Agarose gel electrophoresis study revealed that the metal complexes could cleave super-coiled pBR322 DNA to a nicked form in the absence of external agents. In vitro anti bacterial studies show that copper complex has weak antibacterial activities. Copper complex exhibits a better biological activity among all complexes. This study provides a new perspective and evaluation on the role and importance of the effect factors on the medicinal properties of benzoylthiourea compounds. Synchronous fluorescence spectra of HSA (10 μM) as a function of concentration of the complexes 1-4.

show abstract

Synthesis, Characterization, Antibacterial, DNA Binding and Cleavage Studies of Mixed Ligand Cu(II), Co(II) Complexes

Cited by 10 publications

References 26 publications

Synthesis, structural characterization, antimicrobial, antioxidant and DNA binding studies of some novel homo‐binuclear Schiff base metal (II) complexes

Synthesis, structural characterization, antimicrobial, antioxidant and DNA binding studies of some novel homo‐binuclear Schiff base metal (II) complexes

DNA binding, DNA cleavage and HSA interaction of several metal complexes containing N-(2-hydroxyethyl)-N′-benzoylthiourea and 1,10-phenanthroline ligands

Contact Info

Product

Resources

About