Synthesis, Characterization, and Use of New Solid and Hollow, Magnetic and Non-Magnetic, Organic-Inorganic Monodispersed Hybrid Microspheres

Margel, Shlomo; Gura, Sigalit; Bamnolker, Hanna; Nitzan, B.; Tennenbaum, Tami; Bar-Toov, Beni; Hinz, Michael; Seliger, H.

doi:10.1007/978-1-4757-6482-6_3

Cited by 8 publications

(5 citation statements)

References 20 publications

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“…γ -Fe 2 O 3 magnetic nanoparticles of 15.0 ± 2.1 nm diameter were prepared according to previous publications [32,33]. Briefly, 240 mg of gelatin was dissolved in 80 ml of water at 60 • C. Then, 160 μl of Fe 2+ solution (10 mmol in 5 ml 0.1N HCl) and 57.6 μl of sodium nitrite solution (7.27 mmol in 5 ml H 2 O) were added to the shaken gelatin solution.…”

Section: Synthesis Of the γ -Fe 2 O 3 Nanoparticlesmentioning

confidence: 99%

“…This procedure was repeated successively four more times. The reaction mixture was then shaken at 60 • C for an additional 1 h. The formed γ -Fe 2 O 3 magnetic nanoparticles were then washed from non-magnetic waste with water by the high gradient magnetic field (HGMF) technique [32].…”

Section: Synthesis Of the γ -Fe 2 O 3 Nanoparticlesmentioning

confidence: 99%

“…For example, iron oxide nanoparticles such as Feridex (SPIO; Feridex-USA; Endorem-Europe) have already been FDA-approved as magnetic resonance imaging (MRI) contrast agents for cells of the reticulo-endothelial system [21]. The use of iron oxide magnetic nanoparticles for various biomedical applications e.g., hyperthermia, diagnostic, cell-labeling and sorting, DNA separation, MRI contrast agents and drug delivery has already been demonstrated [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

“…These core-shell nanoparticles were prepared by emulsion polymerization of the fluorinated monomer HFBA in the presence of γ -Fe 2 O 3 nanoparticles [32,33]. Human insulin amyloid fibrils were formed by incubating the monomeric insulin dissolved in an aqueous continuous phase at pH 1.6 and 65 • C.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis and characterization of fluorinated magnetic core–shell nanoparticles for inhibition of insulin amyloid fibril formation

2009

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Maghemite (gamma-Fe2O3) magnetic nanoparticles of 15.0 +/- 2.1 nm are formed by nucleation followed by controlled growth of maghemite thin films on gelatin-iron oxide nuclei. Uniform magnetic gamma-Fe2O3/poly (2,2,3,3,4,4,4-heptafluorobutyl acrylate) (gamma-Fe2O3/PHFBA) core-shell nanoparticles are prepared by emulsion polymerization of the fluorinated monomer 2,2,3,3,4,4,4-heptafluorobutyl acrylate (HFBA) in the presence of the maghemite nanoparticles. The kinetics of the insulin fibrillation process in the absence and in the presence of the gamma-Fe2O3/PHFBA core-shell nanoparticles are elucidated. A significant direct slow transition from alpha-helix to beta-sheets during insulin fibril formation is observed in the presence of the gamma-Fe2O3/PHFBA nanoparticles. This is in contradiction to our previous manuscript, which illustrated that the gamma-Fe2O3 core nanoparticles do not affect the kinetics of the formation of the insulin fibrils, and to other previous publications that describe acceleration of the fibrillation process by using various types of nanoparticles. These core-shell nanoparticles may therefore be also useful for the inhibition of conformational changes of other amyloidogenic proteins that lead to neurodegenerative diseases such as Alzheimer's, Parkinson's, Huntington's, mad cow and prion diseases.

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Section: Synthesis Of the γ -Fe 2 O 3 Nanoparticlesmentioning

confidence: 99%

Section: Synthesis Of the γ -Fe 2 O 3 Nanoparticlesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synthesis and characterization of fluorinated magnetic core–shell nanoparticles for inhibition of insulin amyloid fibril formation

2009

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“…To test for possible evidence of interparticle interactions, studies have been conducted which measure the concentration eects of monodisperse (diameter = 2.7 microns) magnetite/maghemite-coated polymeric microspheres with nonmagnetic microspheres (microspheres without any magnetite coating). A complete description and evaluation of these magnetic microspheres has been previously published (Margel et al, 1997: Moore et al, 2000.…”

Section: Cell Concentration Effects On Mobilitymentioning

confidence: 99%

Mobility measurements of immunomagnetically labeled cells allow quantitation of secondary antibody binding amplification

McCloskey

Comella

Chalmers

et al. 2001

Biotech & Bioengineering

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Magnetic cell separation methods commonly utilize paramagnetic materials conjugated to antibodies that target specific cell surface molecules. The amount of magnetic material bound to a cell is directly proportional to the magnetophoretic mobility of that cell. A mathematical model has been developed which characterizes the fundamental parameters controlling the amount of magnetic material bound, and thus, the magnetophoretic mobility of an immunomagnetically labeled cell. In characterization of the paramagnetic labeling, one of the parameters of interest is the increase in magnetophoretic mobility due to the secondary antibody binding to multiple epitopes on the primary antibody, referred to as the "secondary antibody binding amplification," Psi. Secondary antibody-binding amplification has been investigated and quantitated by comparing the mobilities of lymphocytes directly labeled with anti-CD4 MACS (Miltenyi Biotec, Auburn, CA) magnetic nanoparticle antibody with the mobilities of lymphocytes from the same sample labeled with two different indirect antibody-labeling schemes. Each indirect labeling scheme incorporated a primary mouse anti-CD4 FITC antibody that provides both FITC and mouse-specific binding sites for two different secondary antibody-magnetic nanoparticle conjugates: either anti-FITC MACS magnetic nanoparticle antibody or anti-mouse MACS magnetic nanoparticle antibody. The magnetophoretic mobilities of the immunomagnetically labeled cells were obtained using Cell Tracking Velocimetry (CTV). The results indicate that an average of 3.4 anti-FITC MACS magnetic nanoparticle antibodies bind to each primary CD4 FITC antibody, Psi(1,2f) = 3.4 +/- 0.33, and that approximately one, Psi(1,2m) = 0.98 +/- 0.081, anti-mouse MACS magnetic nanoparticle antibody binds to each primary mouse CD4 FITC antibody on a CD4 positive lymphocyte. These results have provided a better understanding of the antibody-binding mechanisms used in paramagnetic cell labeling for magnetic cell separation.

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Magnetic Nanoparticles and Biosciences

Šafařı́k

Šafařı́ková²

2002

Nanostructured Materials

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Synthesis, Characterization, and Use of New Solid and Hollow, Magnetic and Non-Magnetic, Organic-Inorganic Monodispersed Hybrid Microspheres

Cited by 8 publications

References 20 publications

Synthesis and characterization of fluorinated magnetic core–shell nanoparticles for inhibition of insulin amyloid fibril formation

Synthesis and characterization of fluorinated magnetic core–shell nanoparticles for inhibition of insulin amyloid fibril formation

Mobility measurements of immunomagnetically labeled cells allow quantitation of secondary antibody binding amplification

Magnetic Nanoparticles and Biosciences

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