Synthesis, characterization, and preliminary in vitro antibacterial evaluation of ZnO nanoparticles derived from soursop (Annona muricata L.) leaf extract as a green reducing agent

Selvanathan, Vidhya; Aminuzzaman, Mohammod; Tan, Lee Xian; Win, Yip Foo; Cheah, Eddy Seong Guan; Heng, Mei Hsuan; Tey, Lai-Hock; Arullappan, Sangeetha; Algethami, Norah; Alharthi, Sami S.; Sultana, Sabiha; Shahiduzzaman, Md.; Abdullah, Huda; Aktharuzzaman, Md.

doi:10.1016/j.jmrt.2022.08.028

Cited by 34 publications

(9 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The small particle size that is found in the nanomaterial size range shows a tremendous increase in specific surface area with low dimensions, which results in a wider range and efficient application in a variety of fields . Among semiconducting nanometal oxide-based materials are zinc oxide (ZnO) NPs, which are some of the most effective transition metal oxides that exhibit high antibacterial activity and have been widely used for the detection and quantification of toxic molecules found within different components, such as drugs, foods, drinking matter, soaps, and many others . This is due to their low cost, nontoxicity to eukaryotic cells at therapeutic dosages, low electric resistance, good stability, high catalytic efficiency, high UV protection, excellent electron transfer capability, and, at the same time, biocompatibility properties. − …”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and Optimization of ZnO Nanoparticles Using Ocimum lamifolium Leaf Extract for Electrochemical Sensor and Antibacterial Activity

2023

View full text Add to dashboard Cite

In this study, zinc oxide nanoparticles (ZnO NPs) were synthesized using an aqueous extract of the Ocimum lamifolium (O. lamifolium) plant. The I-optimal coordinate exchange randomized response surface methodology (RSM) was used to optimize the effect of the zinc acetate precursor, temperature, and time on ZnO NPs by designing nine runs. From ANOVA analysis, the significance and validity of the designed model showed that the optimal values of the zinc acetate precursor, temperature, and time during ZnO NPs synthesis were found to be ∼0.06 M, ∼30 °C, and ∼1.35 h, respectively. The obtained ZnO NPs under these optimized conditions were characterized and explored by UV–vis, TGA/DTA, FTIR, XRD, SEM-EDX, TEM, HRTEM, and SAED. Furthermore, the electrocatalytic performance of ZnO NPs was performed for sulfamethoxazole (SMZ) sensing activity with a 0.3528 μM (S/N = 3) limit of detection (LOD). In addition, an antibacterial study revealed that ZnO NPs confirmed an excellent zone of inhibition against E. coli, S. aureus, P. aeruginosa, and S. pyogen pathogenic drug resistance bacterial strains at concentrations of 50, 75, and 100 μg/mL. Thus, ZnO NPs synthesized using the O. lamifolium leaf have a potential electrocatalytic activity for diverse organic pollutant detection as well as a desirable material for such drug resistance antimicrobial strains.

show abstract

Section: Introductionmentioning

confidence: 99%

Biosynthesis and Optimization of ZnO Nanoparticles Using Ocimum lamifolium Leaf Extract for Electrochemical Sensor and Antibacterial Activity

2023

View full text Add to dashboard Cite

show abstract

“…The mechanism of biosynthesis of ZnO NPs using R. coriaria fruit aqueous extract remains unclear. In the literature, two synthesis mechanisms have been proposed for the eco-friendly synthesis of ZnO NPs using plant extracts 41 , 42 . The schematic reaction mechanism for the green synthesis of ZnO NPs is presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

Green synthesis of zinc oxide nanoparticles using Rhus coriaria extract and their anticancer activity against triple-negative breast cancer cells

Mongy,

Shalaby

2024

Sci Rep

View full text Add to dashboard Cite

The growing interest in using plant extracts for the biogenic synthesis of zinc oxide nanoparticles (ZnO NPs) stems from their facile, eco-friendly, and biologically safe approach instead of chemical routes. For the first time, ZnO NPs were successfully biosynthesized using Rhus coriaria fruit aqueous extract as a reducing and capping agent. Characterization revealed that the biosynthesized ZnO NPs possessed a maximum absorbance of approximately 359 nm and closely resembled the hexagonal ZnO wurtzite crystalline structure, with an average crystalline size of 16.69 nm. The transmission electron microscope (TEM) showed the presence of spherical and hexagonal morphologies, with an average grain size of 20.51 ± 3.90 nm. Moreover, the elemental composition of the synthesized ZnO NPs was assessed via energy-dispersive X-ray spectrometry (EDX), and the presence of phytocompounds on their surface was subsequently verified through FT-IR analysis. The ζ-potential of ZnO NPs was recorded at − 19.9 ± 0.1663 mV. Regarding anti-cancer properties, ZnO NPs were found to possess potent anti-tumor effects on MCF-7 and MDA-MB-231 breast cancer cells. Their efficacy was dose-dependent, with IC50 values ranging from 35.04–44.86 μg/mL for MCF-7 and 55.54–63.71 µg/mL for MDA-MB-231 cells. Mechanistic studies in MDA-MB-231 cells revealed apoptosis induction, validated by DAPI staining, confocal microscopy, and Annexin V/PI staining, showing apoptosis by 12.59% and 81.57% at ½ IC50 and IC50 values, respectively. Additionally, ZnO NPs were observed to provoke S-phase arrest and inhibit colony-forming and metastatic potential by modulating apoptosis and metastasis-related genes. This study unravels new insights into how ZnO NPs provoke cancer cell death and inhibit metastasis, revealing new prospects in cancer nanotechnology.

show abstract

“…During chelation, electrons from the precursors' zinc and copper atoms were donated to form positively charged zinc(II) and copper(II) ions, respectively, which then formed metal complexes with the phytochemicals. These metal complexes subsequently bonded with negatively charged oxygen(II) ions during calcination [61]. Another possible mechanism for the formation of ZnO-CuO NCs was bio reduction, where the divalent oxidation state of zinc and copper were reduced to a zero-valent state by the phytochemicals present in the mangosteen leaf aqueous extract, as indicated by the immediate color change during the green synthesis [62].…”

Section: Discussionmentioning

confidence: 99%

“…Another possible mechanism for the formation of ZnO-CuO NCs was bio reduction, where the divalent oxidation state of zinc and copper were reduced to a zero-valent state by the phytochemicals present in the mangosteen leaf aqueous extract, as indicated by the immediate color change during the green synthesis [62]. ZnO and CuO nuclei were formed after the metallic zinc and copper reacted with the dissolved oxygen in the precursor solution [61], and coordinate covalent bonds were subsequently formed between ZnO and CuO through the lone-pair electron from the oxygen atoms of the metal oxides. A strong framework of ZnO-CuO NCs was then produced during calcination [6].…”

Section: Discussionmentioning

confidence: 99%

Impact of Diverse Parameters on the Physicochemical Characteristics of Green-Synthesized Zinc Oxide–Copper Oxide Nanocomposites Derived from an Aqueous Extract of Garcinia mangostana L. Leaf

Chan,

Aminuzzaman,

Tey

et al. 2023

Materials

Self Cite

View full text Add to dashboard Cite

Compared to conventional metal oxide nanoparticles, metal oxide nanocomposites have demonstrated significantly enhanced efficiency in various applications. In this study, we aimed to synthesize zinc oxide–copper oxide nanocomposites (ZnO-CuO NCs) using a green synthesis approach. The synthesis involved mixing 4 g of Zn(NO3)2·6H2O with different concentrations of mangosteen (G. mangostana) leaf extract (0.02, 0.03, 0.04 and 0.05 g/mL) and 2 or 4 g of Cu(NO3)2·3H2O, followed by calcination at temperatures of 300, 400 and 500 °C. The synthesized ZnO-CuO NCs were characterized using various techniques, including a UV-Visible spectrometer (UV-Vis), photoluminescence (PL) spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, X-ray powder diffraction (XRD) analysis and Field Emission Scanning Electron Microscope (FE-SEM) with an Energy Dispersive X-ray (EDX) analyzer. Based on the results of this study, the optical, structural and morphological properties of ZnO-CuO NCs were found to be influenced by the concentration of the mangosteen leaf extract, the calcination temperature and the amount of Cu(NO3)2·3H2O used. Among the tested conditions, ZnO-CuO NCs derived from 0.05 g/mL of mangosteen leaf extract, 4 g of Zn(NO3)2·6H2O and 2 g of Cu(NO3)2·3H2O, calcinated at 500 °C exhibited the following characteristics: the lowest energy bandgap (2.57 eV), well-defined Zn-O and Cu-O bands, the smallest particle size of 39.10 nm with highest surface area-to-volume ratio and crystalline size of 18.17 nm. In conclusion, we successfully synthesized ZnO-CuO NCs using a green synthesis approach with mangosteen leaf extract. The properties of the nanocomposites were significantly influenced by the concentration of the plant extract, the calcination temperature and the amount of precursor used. These findings provide valuable insights for researchers seeking innovative methods for the production and utilization of nanocomposite materials.

show abstract

Synthesis, characterization, and preliminary in vitro antibacterial evaluation of ZnO nanoparticles derived from soursop (Annona muricata L.) leaf extract as a green reducing agent

Cited by 34 publications

References 36 publications

Biosynthesis and Optimization of ZnO Nanoparticles Using Ocimum lamifolium Leaf Extract for Electrochemical Sensor and Antibacterial Activity

Biosynthesis and Optimization of ZnO Nanoparticles Using Ocimum lamifolium Leaf Extract for Electrochemical Sensor and Antibacterial Activity

Green synthesis of zinc oxide nanoparticles using Rhus coriaria extract and their anticancer activity against triple-negative breast cancer cells

Impact of Diverse Parameters on the Physicochemical Characteristics of Green-Synthesized Zinc Oxide–Copper Oxide Nanocomposites Derived from an Aqueous Extract of Garcinia mangostana L. Leaf

Contact Info

Product

Resources

About