Synthesis, Characterization, and Nanodisc formation of Non-ionic Polymers

Ravula, Thirupathi; Ramamoorthy, Ayyalusamy

doi:10.1101/2021.01.11.426267

Cited by 1 publication

(1 citation statement)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chemically-modified inulin (high molecular weight fructo-oligosaccharides (FOS) or fructans) with pentyl, benzyl, hexyl, have been shown to effectively solubilized synthetic lipid bilayers composed of DMPC:DPMG (7:3) and form particles with ~10 nm hydrodynamic radius. These glycopolymers are remarkably tolerant to up to 100 mM divalent metal ions, and fully soluble pH range 2.5–8 [ 40 ].…”

Section: Natural Biopolymers As Tools For Spontaneous Reconstruction Of Biomembrane Assembliesmentioning

confidence: 99%

Current Developments in Native Nanometric Discoidal Membrane Bilayer Formed by Amphipathic Polymers

2021

View full text Add to dashboard Cite

Unlike cytosolic proteins, membrane proteins (MPs) are embedded within the plasma membrane and the lipid bilayer of intracellular organelles. MPs serve in various cellular processes and account for over 65% of the current drug targets. The development of membrane mimetic systems such as bicelles, short synthetic polymers or amphipols, and membrane scaffold proteins (MSP)-based nanodiscs has facilitated the accommodation of synthetic lipids to stabilize MPs, yet the preparation of these membrane mimetics remains detergent-dependent. Bio-inspired synthetic polymers present an invaluable tool for excision and liberation of superstructures of MPs and their surrounding annular lipid bilayer in the nanometric discoidal assemblies. In this article, we discuss the significance of self-assembling process in design of biomimetic systems, review development of multiple series of amphipathic polymers and the significance of these polymeric “belts” in biomedical research in particular in unraveling the structures, dynamics and functions of several high-value membrane protein targets.

show abstract

Section: Natural Biopolymers As Tools For Spontaneous Reconstruction Of Biomembrane Assembliesmentioning

confidence: 99%