Synthesis, characterization and immunogenicity of silk fibroin-l-asparaginase bioconjugates

Synthesis, physicochemical and immunological properties of oxidized inulin–l-asparaginase bioconjugate

“…This condition is probably due to the participation of more reactive aldehyde groups at higher concentration of oxidized inulin, and to increasing steric hindrance by conjugated branched inulins that might interfere with enzyme and substrate reaction (Vina et al, 2001). Other investigators have reported similar results by using different molecules binding to l-ASNase including dextran sulfate (Karsakevich et al, 1986), chitosan (Qian et al, 1996), polyethylene glycol (Soares et al, 2002), levan (Vina et al, 2001), silk sericin (Zhang et al, 2004) and silk fibroin (Zhang et al, 2005).…”

“…One approach to improve the function and properties of the enzyme is its chemical modification with various kinds of polymers. l-ASNase was successfully modified with natural and artificial soluble and insoluble polymers such as albumin (Poznansky et al, 1982), dextran (Karsakevich et al, 1986), polyethylene glycol (PEG) (Soares et al, 2002), chitosan (Qian et al, 1996), silk fibroin (Zhang et al, 2005) and sericine (Zhang et , 2004). PEG-ASNase or pegaspargase is a bioconjugated form of ASNase that has been commercially available since 1994.…”

Electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) for endothelial cell growth

“…The natural polymers could satisfy the biological properties intrinsic in the biomimetic approach. In particular, silk fibroin (SF) seems to be one of the most promising natural polymers for use in biomedical applications due to its several unique properties including good biocompatibility, good oxygen and water vapor permeability, biodegradability, low inflammatory response and commercial availability at relatively low cost [13][14][15]. Moreover, the amino acid sequences of SF molecule can enhance cell adhesion and activity.…”

Different properties of electrospun fibrous scaffolds of separated heavy-chain and light-chain fibroins of Bombyx mori

“…Electrospun fibrous scaffolds are easily produced from various synthetic and natural polymers, whose surfaces were feasibly modified with bioactive molecules to gain acquired biological properties. For these reasons, fabrication of electrospun fibrous scaffolds that mimics morphological, structural and functional properties of natural extracellular matrix (ECM) has been received a lot of interests in tissue engineering and wound healing researches [2,3]. Among many natural polymers, fibroin from silk threads produced by silkworm (Bombyx mori) has received more interests in its biomedical and biological applications, due to its low immunogenicity, good biocompatibility and biodegradability [4][5][6][7].…”