Synthesis, characterization andin vitroevaluation of exquisite targeting SPIONs–PEG–HER in HER2+ human breast cancer cells

Abstract

A stable, biocompatible and exquisite SPIONs-PEG-HER targeting complex was developed. Initially synthesized superparamagnetic iron oxide nanoparticles (SPIONs) were silanized using 3-aminopropyltrimethoxysilane (APS) as the coupling agent in order to allow the covalent bonding of polyethylene glycol (PEG) to the SPIONs to improve the biocompatibility of the SPIONs. SPIONs-PEG were then conjugated with herceptin (HER) to permit the SPIONs-PEG-HER to target the specific receptors expressed over the surface of the HER2+ metastatic breast cancer cells. Each preparation step was physico-chemically analyzed and characterized by a number of analytical methods including AAS, FTIR spectroscopy, XRD, FESEM, TEM, DLS and VSM. The biocompatibility of SPIONs-PEG-HER was evaluated in vitro on HSF-1184 (human skin fibroblast cells), SK-BR-3 (human breast cancer cells, HER+), MDA-MB-231 (human breast cancer cells, HER-) and MDA-MB-468 (human breast cancer cells, HER-) cell lines by performing MTT and trypan blue assays. The hemolysis analysis results of the SPIONs-PEG-HER and SPIONs-PEG did not indicate any sign of lysis while in contact with erythrocytes. Additionally, there were no morphological changes seen in RBCs after incubation with SPIONs-PEG-HER and SPIONs-PEG under a light microscope. The qualitative and quantitative in vitro targeting studies confirmed the high level of SPION-PEG-HER binding to SK-BR-3 (HER2+ metastatic breast cancer cells). Thus, the results reflected that the SPIONs-PEG-HER can be chosen as a favorable biomaterial for biomedical applications, chiefly magnetic hyperthermia, in the future.

Synthesis and Characterization of Folate‐decorated Cobalt Ferrite Nanoparticles Coated with Poly(Ethylene Glycol) for Biomedical Applications

“…Then, the mixture was added to 15 mL of DI water at room temperature and kept for 24 h. Finally, the mixture was purified by dialysis against DI water. The dialysis procedure was repeated five times at 4‐h intervals . The final product was called CFNs@PEG.…”

“…Magnetic nanomaterials have attracted the attention of many researchers owing to their magnetic properties and relatively small size, which have made them excellent candidates for biomedical applications . Besides this, adequate biocompatibility, low cytotoxicity, and colloidal stability are the basic requirements for such materials to be optimally used in biomedical applications .…”

“…The physicochemical properties of coated nanoparticles are affected by the changes in the PEG's molecular weight. Biocompatibility of iron oxide nanoparticles increases when coated with PEG with molecular weight ( M r ) of 5000 in comparison with that with lower molecular weight; however, the physicochemical properties of nanoparticles are hardly affected . Therefore, PEG ( M r = 5000) was chosen for coating the nanoparticles in this study.…”

Nanoparticles as Theranostic Vehicles in Experimental and Clinical Applications—Focus on Prostate and Breast Cancer

“…Specifically, for PCa and BC there is ongoing research on different applications using these kind of NPs [93,104,105,106,107,108,109,110,111,112,113,114,115]. For example, regarding PCa, Zhu et al have performed synthesis, characterization, an in vitro binding assay, and an in vivo magnetic resonance imaging (MRI) evaluation of prostate specific membrane antigen (PSMA) targeting SPIONs.…”

A comparison inhibitory effects of cisplatin and MNPs‐PEG‐cisplatin on the adhesion capacity of bone metastatic breast cancer

“…Recently, one promising approach for overcoming aforesaid disadvantages has been using nanoparticles for carrying the drug out specifically through the cancer cells . For instance, nanoparticles show great promise in cancer therapy because they can selectively gain access to tumors due to their surface modifiability and small size .…”