Synthesis, characterization, and in vitro biocompatibility study of novel disulfide cross‐linked hydrogels based on poly(amic acid)

Abstract

In this study, novel disulfide cross-linked hydrogels were designed and synthesized. First, ethylenediaminetetraacetic dianhydride reacted with butanediamine and amino-terminated polyethylene glycol via N-acylation reaction to give biocompatible poly(amic acid) (PAA) with pendant carboxyl groups; then, the amino groups of cystamine reacted with carboxyl groups of PAA to generate disulfide cross-linked network polymer (PAA-SS). Fourier transform infrared spectroscopy, 1 H nuclear magnetic resonance imaging, gel permeation chromatography with multiangle laser light scattering, potentiometric titration, rheology, hydrolytic degradation, morphology, porosity, and in vitro biocompatibility studies were used to qualitatively and quantitatively characterize the obtained polymers. The results indicated that the equilibrium swelling ratio of the PAA-SS decreased with the increase in Rm. The PAA-SS provided good mechanical strength to maintain their integrity, and the storage modules (G 0 ) of the hydrogels can be adjusted by Rm. The PAA-SS presented co-continuum pores, and the pore size correlated with the cross-linking degree. The degradation of PAA-SS could be controlled by regulating the concentration of dithiothreitol. Particularly, the PAA-SS possessed an excellent biocompatibility, as the average proliferating rate of osteoblasts on PAA-SS was appreciably higher than that on PAA and glass coverslips. In conclusion, the above obtained results demonstrate that the performance of the PAA-SS outbalance and facilitate the application in biomedical region, particularly in bone tissue regeneration.