Synthesis, Characterization, and Functionalization of Chitosan and Gelatin Type B Nanoparticles to Develop Novel Highly Biocompatible Cell-Penetrating Agents

González, Cristina; Reyes, Luis H.; Muñoz-Camargo, Carolina; Cruz, Juan C.

doi:10.3390/iocn2020-07816

Cited by 4 publications

(5 citation statements)

References 26 publications

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“…Similar results were found for platelet aggregation, where the nanobioconjugates exhibited thrombogenic activities comparable to the negative control (PBS-1X), i.e., they remained at around 50% for concentrations up to 150 µg/mL. Similar hemocompatibility results were also reported by us recently for BUF-II-CNPs nanobioconjugates [74]. However, in a situation where the CNPs-AHB-1 nanobioconjugates were to be employed at concentrations above 300 µg/mL, they should be evaluated further, considering that they start to induce mild platelet activation at such concentration levels and, consequently, the possibility of thrombotic complications [75].…”

Section: Molecular Dynamics Simulationssupporting

confidence: 89%

Translocating Peptides of Biomedical Interest Obtained from the Spike (S) Glycoprotein of the SARS-CoV-2

et al. 2022

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At the beginning of 2020, the pandemic caused by the SARS-CoV-2 virus led to the fast sequencing of its genome to facilitate molecular engineering strategies to control the pathogen’s spread. The spike (S) glycoprotein has been identified as the leading therapeutic agent due to its role in localizing the ACE2 receptor in the host’s pulmonary cell membrane, binding, and eventually infecting the cells. Due to the difficulty of delivering bioactive molecules to the intracellular space, we hypothesized that the S protein could serve as a source of membrane translocating peptides. AHB-1, AHB-2, and AHB-3 peptides were identified and analyzed on a membrane model of DPPC (dipalmitoylphosphatidylcholine) using molecular dynamics (MD) simulations. An umbrella sampling approach was used to quantify the energy barrier necessary to cross the boundary (13.2 to 34.9 kcal/mol), and a flat-bottom pulling helped to gain a deeper understanding of the membrane’s permeation dynamics. Our studies revealed that the novel peptide AHB-1 exhibited comparable penetration potential of already known potent cell-penetrating peptides (CPPs) such as TP2, Buforin II, and Frenatin 2.3s. Results were confirmed by in vitro analysis of the peptides conjugated to chitosan nanoparticles, demonstrating its ability to reach the cytosol and escape endosomes, while maintaining high biocompatibility levels according to standardized assays.

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Section: Molecular Dynamics Simulationssupporting

confidence: 89%

Translocating Peptides of Biomedical Interest Obtained from the Spike (S) Glycoprotein of the SARS-CoV-2

et al. 2022

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“…In chitosan, the positively charged groups such as the amino groups result in net positive zeta potential. 33 , 34 …”

Section: Resultsmentioning

confidence: 99%

“…The carboxylic acid groups in gelatin cause a decrease in zeta potential. In chitosan, the positively charged groups such as the amino groups result in net positive zeta potential. , …”

Section: Resultsmentioning

confidence: 99%

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Crosslinked Chitosan–Gelatin Biocompatible Nanocomposite as a Neuro Drug Carrier

Mathew

2022

ACS Omega

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The polymers, chitosan, a polysaccharide, and gelatin, a protein, are crosslinked in different ratios without the aid of a crosslinking agent. Facile chemical reactions were followed to synthesize a chitosan/gelatin nanocomposite in three different ratios (1:1, 1:3, and 3:1). The solubility of chitosan and the stability of gelatin were improved due to the crosslinking. Both the polymers have excellent biodegradability, biocompatibility, adhesion, and absorption properties in a biological environment. The properties of the composite were favorable to be used in drug delivery applications, and the drug dopamine was encapsulated in the composite for all three ratios. The properties of the chitosan/gelatin nanocomposite and dopamine-loaded chitosan/gelatin nanocomposite were examined using XRD, FTIR, SEM, UV, TGA, TEM, and DLS techniques, and the crosslinking was confirmed. Higuchi kinetic release was seen with a cumulative release of 93% within 24 h for the 1:3 nanocomposite in a neutral medium. The peaks at 9 and 20° in the XRD spectrum confirmed the encapsulation of dopamine with the increase in the crystallinity of chitosan, which is also evident from the SAED image. The dopamine functional groups were confirmed from the IR peaks between 500 and 1500 cm –1 and the wide UV absorption maxima between 250 and 290 nm. The particle size of the drug-loaded composite in the ratios 1:1, 1:3, and 3:1 were calculated to be 275, 405, and 355 nm, respectively. The nanocomposite also showed favorable DPPH antioxidant and antibacterial activity against Staphylococcus aureus . Sustained release of dopamine in a neutral medium using crosslinked chitosan and gelatin without the presence of a crosslinker is the highlight of the work.

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“…The outputs are in good agreement with DLS results. González et al [17] have shown the morphology of CS NPs through low molecular weight by TEM analysis that has the size of individual particles was 5 to 10 nm.…”

Section: Preparation and Characterization Of Npsmentioning

confidence: 99%

Improved Mucoadhesive Properties of Repaglinide-Loaded Nanoparticles: Mathematical Modelling through Machine Learning-Based Approach

Namazi

2023

Indones. J. Chem.

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This research work aims to develop a modified repaglinide-loaded chitosan-ethyl cellulose nanoparticles (RPG-ECSNPs) as a novel sustained-release dosage form with improved mucoadhesive properties using an emulsification solvent-evaporation technique. The RPG-ECSNPs with different particle sizes were prepared from various polymers containing ethyl cellulose (EC) as the internal phase and chitosan (CS) as the external phase, and the use of surfactants, including Tween 80 and poloxamer 188 as emulsifiers. In vitro drug release, drug loading amount, and entrapment efficiency have been influenced by changes in the concentrations of CS and EC. The mean droplet size and zeta potential of RPG-ECSNPs were 213 ± 8.5 nm and 16.4 ± 2.4 mV, respectively. The optimized formulation's entrapment efficiency was 66 ± 2.3%, and drug loading was 7.9 ± 1.65%. The release profile was significantly higher in PBS (90%) than in diluted hydrochloric acid (30%) during 24 h of the study. The mucoadhesive function of the particles was examined in vitro using part of rat intestines. The highest adhesive % was observed for the chitosan-coated NPs. No adhesive properties were noticed for chitosan-free NPs (P-value > 0.05). This indicated that ECSNPs can be successfully utilized for sustained and controlled drug delivery of RPG through the GIT.

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Synthesis, Characterization, and Functionalization of Chitosan and Gelatin Type B Nanoparticles to Develop Novel Highly Biocompatible Cell-Penetrating Agents

Cited by 4 publications

References 26 publications

Translocating Peptides of Biomedical Interest Obtained from the Spike (S) Glycoprotein of the SARS-CoV-2

Translocating Peptides of Biomedical Interest Obtained from the Spike (S) Glycoprotein of the SARS-CoV-2

Crosslinked Chitosan–Gelatin Biocompatible Nanocomposite as a Neuro Drug Carrier

Improved Mucoadhesive Properties of Repaglinide-Loaded Nanoparticles: Mathematical Modelling through Machine Learning-Based Approach

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