Synthesis, characterization and fluorescent properties of cerium(III) glutathione complex

Abstract

The cerium (III) glutathione complex was synthesized by the redox reaction of cerium (IV) with glutathione reduced (GSH) in aqueous solution. The Job-plots indicate an ML (L = GSSG) stoichiometry of the complex. The fluorescent properties of the compound were investigated. The as-prepared complex showed the characteristic maximum emission spectra of Ce(III) at 350 nm (λ(ex) = 255 nm). The fluorescence results show that the Ce(IV) ions are first reduced to Ce(III), and then form Ce(III) complex after reacting with GSH. The complex was characterized by element analysis and FT-IR spectra; the stability of the complex was analyzed by cyclic voltammeters and DSC-TG as well. Finally, Ce(IV) was successfully employed to determine the concentrations of GSH in the presence of GSSG, in which the fluorescence intensities are proportional to the concentrations of GSH in the range of 1-100 nM with the detection limit of 0.05 nM of GSH, without interference from the presence of GSSG.

Redox Reactivity of Cerium Oxide Nanoparticles Induces the Formation of Disulfide Bridges in Thiol-Containing Biomolecules

“…For these experiments, cysteine was used as a model. Han's experiments were adapted to detect Ce 3+ −disulfide complexes at pH 6.0−7.4, 47 and a lack of experimental artifacts was verified (Figure S5a,b, Supporting Information)…”

“…40 The possible reaction mechanism can be described as the formation of a disulfide bridge−Ce (III) complex after interaction between Ce(IV) and the thiol groups. 47 This interesting property was used here to show the formation of complexes between cerium and disulfide groups after interaction with pristine CeO 2 NPs. For these experiments, cysteine was used as a model.…”

Rapid and accurate tumor-target bio-imaging through specific in vivo biosynthesis of a fluorescent europium complex

“…Recently, it has been reported that the cerium(III) glutathione complex can be synthesized through the redox reaction of cerium(IV) with GSH reduced by chemical synthesis in aqueous solution. 27 Because of its similar electron structure with cerium, the chemical properties of europium are analogous to cerium. In addition, the p53 signaling pathway may be involved in the production of NADPH, which can readily catalyze the oxidative decarboxylation of malate to generate NADPH or NADP +39 (Fig.…”

“…Glutathione as a bioactive tripeptide contains the reduced GSH and oxidized GSSG and has an important physiological effect in living systems. 27,28 Using these peculiarities, we have realized the special self-biosynthesis of gold and silver nanoclusters (NCs) 29,30 in cancer cells or tumor tissues.…”

Energy Transfer Processes of Chemiluminescence Reaction Systems with Cerium(IV) Ions and Their Analytical Application: A Review

“…In general, emitters of chemiluminescence reaction systems with cerium(IV) ions can be classified into five groups Ce(III) [8], excited sulphur dioxide (SO 2 *), excited products of oxidation of determined substance [18,19], dimols of singlet oxygen [20,35] and fluorophores [6,9,14]. Cerium (III), which is formed by the reduction of cerium(IV), is a well-known fluorescent ion, with the maximum emission at λ~355 nm [41][42][43] and therefore, it is a possible CL emitter. Excited sulphur dioxide species (SO 2 *) emit weak light at 300-450 nm [44,45], therefore the compounds showing strong fluorescence, such as rhodamine B [28,46], quinine [29][30][31] or lanthanide ions [32] are introduced to many reaction systems containing Ce(IV) ions.…”