Synthesis, characterization, and evaluation of antibacterial efficacy of rhamnolipid-coated zinc oxide nanoparticles against Staphylococcus aureus

Malakar, Chandana; Patowary, Kaustuvmani; Deka, Suresh; Kalita, Mohan Chandra

doi:10.1007/s11274-021-03160-w

Cited by 12 publications

(8 citation statements)

References 36 publications

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“…The in vitro antibacterial efficacy of ZnRL has been reported previously by Malakar et al 14 In this study, the in vivo antibacterial efficacy of 100 μl of 0.5 mg/ml ZnRL and rhamnolipid has been evaluated. As could be observed in Figure 3A, the Mueller Hinton agar plates spread with 100 μl of skin scraping revealed the presence of a large quantity of the pathogen in the wound site of the infected untreated wounds on the 5th day of infection.…”

Section: Culture Recoverymentioning

confidence: 67%

“…The non-cytotoxicity of the nanoparticles has been previously reported in Malakar et al 14 The impact of the nanoparticle on the fibroblast cells at a concentration of 0.5 mg/ml was observed under a microscope. It was observed that normal cells grow efficiently in absence of any treatment.…”

Section: Cell Observationmentioning

confidence: 69%

“…The effective invitro antibacterial activity of the rhamnolipid‐coated zinc oxide nanoparticles has been established in Malakar et al 14 At a concentration of 0.5 mg/ml, ZnRL exhibited 90% growth inhibition against the pathogen S.aureus .…”

Section: Resultsmentioning

confidence: 99%

“…For the production of rhamnolipid, P.aeruginosa was obtained from the germplasm of IASST. The production and purification of rhamnolipid was performed according to Malakar et al 14 …”

Section: Methodsmentioning

confidence: 99%

“…The production of rhamnolipid‐coated zinc oxide nanoparticles has been carried out as described by Malakar et al 14 …”

Section: Methodsmentioning

confidence: 99%

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Wound healing efficacy of rhamnolipid‐coated zinc oxide nanoparticle along with its in vivo antibacterial efficacy against Staphylococcus aureus

Malakar

Kashyap

Kalita

et al. 2022

Experimental Dermatology

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Rhamnolipids are microbial metabolites with antibacterial efficacies, which can be further boosted through the application of nanobiotechnology. In this study, the efficacy of rhamnolipid-coated zinc oxide nanoparticles (ZnRL) has been studied for their wound healing efficacy as well as in vivo antibacterial efficacy. Thus, this study evaluates the efficacy of ZnRL to heal an excised infected wound, which was compared with the healing efficacy of rhamnolipid and clindamycin. The study revealed that rhamnolipid-coated zinc oxide nanoparticles possess promising wound healing efficacy with prominent antibacterial activity in the rat model. Prominent wound healing in a Staphylococcus aureus infected excised wound was observed on the 5th day of the treatment when the wound site was treated with 100 μl of 0.5 mg/ml of ZnRL. This concentration of ZnRL was found to exhibit efficient antibacterial activity against the pathogen, thereby decreasing the amount of pathogen in the wound site. ZnRL exhibited efficient wound contraction, thereby decreasing the size of the wound prominently in 5 days. Histological study revealed efficient tissue remodelling in ZnRL-treated skin which resulted in rapid formation of the epidermis and recruitment of various dermal cells within the 5th day of treatment. The study also revealed the non-cytotoxic effect of the nanoparticles in fibroblast cell line L929 and the nonhaemolytic effect against blood cells, indicating its potential in pharmaceuticals.

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Section: Culture Recoverymentioning

confidence: 67%

Section: Cell Observationmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

“…For the production of rhamnolipid, P.aeruginosa was obtained from the germplasm of IASST. The production and purification of rhamnolipid was performed according to Malakar et al 14 …”

Section: Methodsmentioning

confidence: 99%

“…The production of rhamnolipid‐coated zinc oxide nanoparticles has been carried out as described by Malakar et al 14 …”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Wound healing efficacy of rhamnolipid‐coated zinc oxide nanoparticle along with its in vivo antibacterial efficacy against Staphylococcus aureus

Malakar

Kashyap

Kalita

et al. 2022

Experimental Dermatology

Self Cite

View full text Add to dashboard Cite

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A biosurfactant‐producing novel bacterial strain isolated from vermicompost having multiple plant growth‐promoting traits

et al. 2023

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The nutrient-rich vermicompost which is used as manure for the growth and development of plants is rich in microbial flora. These microbes protect the plants against several infectious pathogenic microbes. As certain microbes are known to produce biosurfactants as metabolites, an investigation was carried out to isolate biosurfactant-producing bacterial strains from vermicompost with the efficient antifungal property. From the study, it was revealed that biosurfactant-producing bacterial strains are present in the vermicompost. A total of nine bacterial strains were isolated from the vermicompost. Among them, one most efficient biosurfactant-producing bacterial strains with antifungal properties have been screened. After molecular characterization of the isolated strain, it was revealed that the bacterial strain is Bacillus licheniformis strain SCV1. The strain produces 3.4 ± 0.1 g/L of crude biosurfactant, which when column purified yields 3.1 ± 0.1 g/L of biosurfactant. The biosurfactant exhibited excellent emulsifying activity (E 24 ) of 96.56% against crude oil. The produced biosurfactant was identified as a lipopeptide consisting of a mixer of surfactin and iturin. Furthermore, the biosurfactant exhibited significant antifungal activity against a wide range of phytopathogens, showing 76.3% inhibition against Sclerotinia sclerotiorum, 53% inhibition against Colletotrichum gloeosporioides, 51% against Fusarium verticillioides, and 36% against Corynespora cassicolla. Along with antifungal activities, the stain was found to exhibit multiple plant growth-promoting traits. This study, thus indicates that vermicompost might contain biosurfactant-producing microbes which can render protection to the plant against various phytopathogens by the production of biosurfactants and can also stimulate plant growth.

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Biosynthesis of zinc oxide nanoparticles via endophyte Trichoderma viride and evaluation of their antimicrobial and antioxidant properties

et al. 2022

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Synthesis, characterization, and evaluation of antibacterial efficacy of rhamnolipid-coated zinc oxide nanoparticles against Staphylococcus aureus

Cited by 12 publications

References 36 publications

Wound healing efficacy of rhamnolipid‐coated zinc oxide nanoparticle along with its in vivo antibacterial efficacy against Staphylococcus aureus

Wound healing efficacy of rhamnolipid‐coated zinc oxide nanoparticle along with its in vivo antibacterial efficacy against Staphylococcus aureus

A biosurfactant‐producing novel bacterial strain isolated from vermicompost having multiple plant growth‐promoting traits

Biosynthesis of zinc oxide nanoparticles via endophyte Trichoderma viride and evaluation of their antimicrobial and antioxidant properties

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