Synthesis, Characterization, and Evaluation of the In-vitro Antimicrobial Efficacy of Cinnamomum Zeylanicum Bark-Extract- Mediated

Abstract

Cinnamomum zeylanicum have lot-of biological activities including antimicrobial, antioxidant and antifungal properties. Furthermore, cytotoxic and apoptotic activities of several constituents were identified throughout its biological properties. Bark of Cinnamomum zeylanicum (Lauraceae) collected respectively at Nanotechnology laboratory (ANGRAU, Tirupathi, India). In this study, microbiological aspects of scale formation in PVC pipelines bacteria and fungi were isolated. Stable Zn nanoparticles were formed by treating 90ml of 1mm zinc nitrate aqueous solution with 10ml of 10% bark extract. The formation of Cinnamomum zeylanicum bark extract mediated zinc nanoparticles (CZnNPs) was confirmed by UV-visible spectroscopic analysis and recorded the localized surface plasmon resonance (LSPR) at 270nm. Fourier transform infrared spectroscopic (FT-IR) analysis revealed that primary and secondary amine groups in combination with the proteins present in the bark extract are responsible for the reduction and stabilization of the CZnNPs. The morphology and crystalline phase of the nano crystals were determined by Transmission electron microscopy (TEM) and (XRD). The hydrodynamic diameter (20.3mm) and a positive zeta potential (10.4mV) were measured using the dynamic light scattering technique. The antimicrobial activity of CZnNPs was evaluated (in vitro) against fungi, Gram-negative and Gram-positive bacteria using disc diffusion method which were isolated from the scales formed in drinking water PVC pipelines. Research in Medical & Engineering Sciences 2/3Res Med Eng Sci evaluate the antimicrobial activity (Biofilm degradation in drinking water pipelines) of the aqueous extract towards pathogenic fungi and bacteria by in vitro disc diffusion method. Materials and Methods MaterialsZinc nitrate (& gt; 99% pure) was purchased from Sigma Aldrich, India. Potato dextrose broth, Potato dextrose agar, Nutrient broth, Nutrient agar plate, was supplied by Hi-media, India. Collection of biofilm formed in poly vinyl chloride (PVC) pipes The PVC Biofilm samples were collected from four different regions located in and around Tirupati, (Chittoor District) Andhra Pradesh, India. The samples were collected from drinking water PVC pipelines and taken in the sterile container. These samples were stored in an ice box and transported to the laboratory for microbiological characterization [18]. Collection of plant materialHealthy Cinnamomum zeylanicum stem barks were collected from Tirumala hills, Andhra Pradesh state, India. From the selected plant bark was collected by scrapping the trunk using neat and clean knife during the month of July 2014 and collected material was carefully washed and dried at 45 °C to constant weight. The dried bark of plant material were powdered, passed through a BSS no. 85-mesh sieve and stored in air tight container. Isolation of fungal and bacterial sp. from drinking water pipelineEight fungal species and ten bacterial samples were isolated from drinking water supply PVC pipelines in Tirupati, Chittoor...