Synthesis, Characterization, and Cytotoxicity Evaluation of Gallic Acid Nanoparticles Towards Breast T47D Cancer Cells

Arsianti, Ade; Bahtiar, Anton; Fadilah, Fadilah; Wangsaputra, Vincent Kharisma; Paramita, Rafika Indah; Azizah, Norma Nur; Nadapdap, Lince Dameria; Fajrin, Ajeng Megawati; Tanimoto, Hiroki; Kakiuchi, Kiyomi

doi:10.5530/pj.2020.12.51

Cited by 10 publications

(5 citation statements)

References 17 publications

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“…Based on a literature survey, we tested eighteen phytochemical compounds with reported antiviral properties including, Allicin (Xie et al,2020), Bornyl acetate , Capsaicin (Tang et al, 2020), Carvacrol (Bansal et al, 2020), Catechol (Mishra et al, 2020), Cinnamic acid (Silva et al, 2020), Curcumin (Bonfim et al, 2020), Eucalyptol (Patra et al, 2020), Gallic acid (Arsianti et al, 2020), Geraniol (Wei et al, 2020), Linalool (Del Bosco et al, 2020, Nicotinic acid (Corona et al, 2020), Phytic acid (Narayanaswamy and Esa 2018), Piperine (Chandani et al, 2019), Quercetin (Nile et al, 2020;Lopes et al, 2020;Aggarwa et al, 2020), Resorcinol (Lee et al, 2019), Terpinen-4-ol (Patra et al, 2020) and Viridiflorene (Shayeganmehr et al, 2018). Additionally, we have used as molecular docking controls, two FDA-approved old antimalarial drug, Chloroquine and Hydroxychloroquine (an analogue of chloroquine) recommended for treating COVID-19 patients (Gautret et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Theoretical Molecular Docking Study of the Structural Disruption of the Viral 3CL-Protease of COVID19 Induced by Binding of Capsaicin, Piperine and Curcumin Part 1: A Comparative Study with Chloroquine and Hydrochloroquine Two Antimalaric Drugs

González

Lossada

Moncayo

et al. 2020

Preprint

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The global pandemic caused by infections of the new coronavirus (COVID-19) makes it necessary to find possible less toxic and easily accessible therapeutic agents. In this study, we used strategies docking and molecular dynamics to analyze phytochemical compounds against FDA-approved antimalarial drugs recommended for the treatment of COVID-19. The evaluation was performed with the docking scores MolDock Score and Rerank Score calculated by Molegro Molecular. The DockThor server was used to generate the complexes and myPresto for the dynamic studies. Preliminary results suggested that piperine, capsaicin, and curcumin have the best docking scores and that they are capable of promoting structural changes in the viral protease by inducing folding of the enzyme. Curcumin and capsaicin bring the enzyme to a more compact conformational state compared to the native state, compared to chloroquine. Even though, it is unknown if these induced changes in protease are related to any inhibitory effect observed both in vitro and in vivo for any of these compounds. Further studies on the mechanisms of action of these compounds of interest are required, as well as experimental demonstrations. However, these results are interesting because they can serve as a starting point for subsequent experimental or/and in silico studies based on chemical structure-activity relationships taking these small molecules and their possible derivatives.

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Section: Methodsmentioning

confidence: 99%

Theoretical Molecular Docking Study of the Structural Disruption of the Viral 3CL-Protease of COVID19 Induced by Binding of Capsaicin, Piperine and Curcumin Part 1: A Comparative Study with Chloroquine and Hydrochloroquine Two Antimalaric Drugs

González

Lossada

Moncayo

et al. 2020

Preprint

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“…This creates a purple colour when dissolved in a suitable solvent; the intensity of the colour is related to the number of viable cells, which may be quantified spectrophotometrically at 570nm. [25][26][27][28] Without the test agent, cell culture plates were seeded with 200µl cell suspension in a 96-well plate at the required cell density (20,000 cells per well). The cells were then allowed to grow for 24 hr.…”

Section: Reducing Power Activitymentioning

confidence: 99%

Tinospora cordifolia Stem Extract-mediated Green Synthesis of Selenium Nanoparticles and its Biological Applications

Puri¹,

Patil²

2022

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Background: According to a literature review Tinospora cordifolia possess alkaloids, tannins, flavonoids, glycosides, and steroids with anti-cancer potential. However, there is a lack of scientific evidence on the green synthesis of selenium nanoparticles from the stem of T. cordifolia and its biological activities. Objectives: This study is intended to synthesize Selenium Nanoparticles using T. cordifolia stem extract, characterise them, and evaluate them in-vitro for antioxidant and anticancer activity. Materials and Methods: The T. cordifolia Selenium Nanoparticles (TC-SeNPs) were synthesised by reducing selenious acid with T. cordifolia stem extract. TC-SeNPs were characterized by UV-Vis, FTIR, XRD, FE-SEM, EDAX and ICP-AES, further evaluated for in-vitro antioxidant activity by DPPH free radical scavenging test and reducing power capacity. The MTT assay of TC-SeNP was carried out in-vitro in the human breast cancer cells line (MCF-7) to determine the anticancer activity. Results:The UV-spectroscopy of TC-SeNPs showed maximum absorbance at 285 nm, which is the characteristic feature of SeNP. According to DLS, SEM, and TEM images, the size of TC-SeNPs was between 100-200 nm and was stable with a Zeta potential of -23.9mV. XRD analysis confirmed the crystallinity of TC-SeNPs. Selenium in TC-SeNP was verified in EDAX analysis (63.36% weight) and ICP-AES 68.08 ±12.2 µg/ml selenium content. The IC 50 value 28.62 ± 0.63 µg/mL and EC 50 (62.50 ± 1.21 µg/mL) values indicated that the TC-SeNPs possess significant antioxidant capacity. MTT assay showed TC-SeNPs were cytotoxic to MCF-7 cell with IC 50 values (31.29 ± 0.22µg/mL), suggesting that TC-SeNPs display moderate cytotoxicity that could dose-dependently inhibit cell proliferation. Conclusion: Thus, experimental evidence provides insight into the successful synthesis of the selenium nanoparticle from the stem of T. cordifolia, its potential therapeutic value, and the prospect of developing an anti-cancer TC-SeNPs formulation.

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“…35 Accordingly, preparation of GA in form of NPs is believed to increase its hydrophobicity to be able to diffuse easily through the cancer cell membrane and hence, improve its anticancer activity. 34 Gallic acid exerts its anticarcinogenic effects through a pleiotropic molecular mechanism(s) of action on cell cycle, cell apoptotic processes, angiogenesis, and metastasis. 36 Nonetheless, the primary mechanism of action of GA is attributed to both its antioxidant as well as prooxidant characteristics, displaying a dual-edge sword behavior.…”

Section: Introductionmentioning

confidence: 99%

“…Noteworthy, GA is hydrophilic, causing it difficult to penetrate the wall of cancer cells. 34 This issue has been resolved by using nanotechnology. Nanoparticles can be used as nano-carriers for antioxidants and such NPs that are termed as nano-antioxidants.…”

Section: Introductionmentioning

confidence: 99%

Rationale for Tailoring an Alternative Oncology Trial Using a Novel Gallium-Based Nanocomplex: Mechanistic Insights and Preclinical Challenges

Mostafa

Salem

Mansour

et al. 2022

Technol Cancer Res Treat

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Introduction: In the fight against cancer, cisplatin is most widely used as a clinical mainstay for the chemotherapy of various human cancers. Meanwhile, its cytotoxic profile, as well as drug resistance, limits its widespread application. The goal of precision medicine is to tailor an optimized therapeutic program based on the biology of the disease. Recently, nanotechnology has been demonstrated to be promising in this scenario. Objective: The current work provides a rationale for the design of an alternative oncology trial for the treatment of hepatocarcinogenesis using a novel eco-friendly nanocomplex, namely gallic acid-coated gallium nanoparticles. Moreover, the study tests whether the antineoplastic efficacy of gallic acid-coated gallium nanoparticles could be enhanced or not when it is administrated together with cisplatin. Methods: The work comprised a series of both in vitro and in vivo investigations. The in vivo therapeutic efficacy of such treatments, against diethylnitrosamine-induced hepatocarcinogenesis, was strictly evaluated by tracking target genes expressions, iron homeostasis, diverse biomarkers alterations, and lastly, routine paraclinical investigations were also assessed. Results: The in vitro biological evaluation of gallic acid-coated gallium nanoparticles in a HepG-2 cancer cell line established its superior cytotoxicity. Else more, the results of the in vivo experiment highlighted that gallic acid-coated gallium nanoparticles could diminish key hallmarks of cancer by ameliorating most of the investigated parameters. This was well-appreciated with the histopathological findings of the liver architectures of the treated groups. Conclusions: Our findings suggest that novel biogenic Ga-based nanocomplexes may potentially present new hope for the development of alternative liver cancer therapeutics, which should attract further scientific interest.

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Synthesis, Characterization, and Cytotoxicity Evaluation of Gallic Acid Nanoparticles Towards Breast T47D Cancer Cells

Cited by 10 publications

References 17 publications

Theoretical Molecular Docking Study of the Structural Disruption of the Viral 3CL-Protease of COVID19 Induced by Binding of Capsaicin, Piperine and Curcumin Part 1: A Comparative Study with Chloroquine and Hydrochloroquine Two Antimalaric Drugs

Theoretical Molecular Docking Study of the Structural Disruption of the Viral 3CL-Protease of COVID19 Induced by Binding of Capsaicin, Piperine and Curcumin Part 1: A Comparative Study with Chloroquine and Hydrochloroquine Two Antimalaric Drugs

Tinospora cordifolia Stem Extract-mediated Green Synthesis of Selenium Nanoparticles and its Biological Applications

Rationale for Tailoring an Alternative Oncology Trial Using a Novel Gallium-Based Nanocomplex: Mechanistic Insights and Preclinical Challenges

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