SYNTHESIS, CHARACTERIZATION AND CYCLAZATION OF PYRAN USING Ag2O NANOPARTICLE FROM NATURAL SOURCE “GINGER”

Ruaa, M D; Al-Sahib, Sanaa A.; Ascar, Israa F.

doi:10.36103/ijas.v52i5.1455

Cited by 1 publication

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“…They demonstrated the essential roles that can be exploited in the development of living cells through their unique physical and chemical properties, which allow for improving the vital activities of the plant cell (34). Silver nanoparticles (Ag-NPs) represent one of the available types of nanomaterial, which has attained wide interest from researchers due to its remarkable physiological properties (30,31) including its potential to influence plant cell growth and thus biomass accumulation and production of metabolic compounds (2,5,7). Based on that, this study was aimed to find the optimal method to obtain the highest possible response to callus induction from the cotyledons by testing two culture mediums, including LS and B5, under different combinations of growth regulators 2,4-Dichlorophenoxy acetic acid (2,4-D) and Benzyl adenine (BA) as well as an ability to test culture media (LS or B5) and Ag-NPs as effective tools that enable increased accumulation of plant secondary compounds in vitro.…”

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confidence: 99%

EFFECT OF CHEMICAL MEDIA COMPONENTS AND SILVER NANOPARTICLES ON THE INDUCTION OF CALLUS CULTURE AND ACCUMULATION OF SECONDARY COMPOUNDS IN A Trigonella foenum-graecum L.

Masara D. J.,

S. I. Neamah

2024

Iraqi J. Agric. Sci.

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This study was investigate the effect of components media and silver nanoparticles (Ag-NPs) synthesized in the laboratory on the induction of biomass and secondary metabolites of calli cultures induced from the cotyledons of Trigonella foenum-graecum L. The culture media LS and B5 were tested with the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) at a concentration of 1.0, 2.0 and 3.0 mg l-1 and 6-benzyladenine (BA) at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1, with the aim of studying its effect on callus induction. In comparison, Ag-NPs were used at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1 to study its effect on the production of some secondary compounds. The results showed that the mixture consisting of the culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BA the highest percentage of callus induction and the minimum days to complete callus formation. While, the same media with 2.0 mg l-1 2,4-D and 1.0 mg l-1 of BA recorded the highest dry weight (DW) and relative water content (RWC). The combination B5×2.0 mg l-1 2,4-D×1.5 or 2.0 mg l-1 showed the highest fresh weight (FW) and the lowest time required to start callus induction, respectively. As for the production of SC, the B5 media with 2.0 mg l-1 of Ag-NPs achieved the highest concentration of alkaloid compounds represented by trigonelline and diosgenin, and flavonoids expressed by qurcetine, vitxein, rutin, apigenin, isovitexin, and kaemferol.

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