Synthesis, characterization, and biological applications of semiconducting polythiophene‐based nanoparticles

Zangoli, Mattia; Maria, Francesca Di

doi:10.1002/viw.20200086

Cited by 26 publications

(19 citation statements)

References 162 publications

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“…For analytical speed, only seconds per sample is needed to obtain the raw spectra. For identification capability, MS measures the molecular weight of molecules accurately (within ±10 mDa) and MS/MS provides fragmentation patterns for direct identification [5c, 10c, 24] . Profiling of large biomolecules leads to substantial progress of modern omics, which is based on the success of sequencing in large‐scale application by fragmentation [4a, 25] .…”

Section: Resultsmentioning

confidence: 99%

Nanoparticle‐Assisted Cation Adduction and Fragmentation of Small Metabolites

et al. 2021

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Mass spectrometry (MS) promises small-metabolite profiling as atool of the future and calls for the comprehensive understanding of key procedures to enhance its capability. Herein, we studied cation adduction and fragmentation of small metabolites by ac ombination of theoretical and experimental approaches,via nanoparticle-assisted laser desorption/ ionization (LDI)-MS and MS/MS.W ec alculated the energies of adduction conformers and atomic bond orders to establish the rules of cation-metabolite affinity and multiple cation adductions in charge transfer.F urther,w ed emonstrated the reaction paths of adducted ions and mapped the potential energy surfaces to characterizethe loss of given groups during fragmentation. Finally,w es uccessfully controlled metabolite fragmentation by selected and multiple adductions to enhance the atomic/fragment coverage as defined for metabolite identification toward profiling.C onsidering the success of MS in the analysis of large biomolecules,o ur work may have an impact and guide to advanced analysis of small metabolites.

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Section: Resultsmentioning

confidence: 99%

Nanoparticle‐Assisted Cation Adduction and Fragmentation of Small Metabolites

et al. 2021

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“…All polymers, which are processable in common organic solvents, can be easily obtained as stable suspensions of NPs in water by using the reprecipitation method. [ 32,33 ] Our results demonstrate that these engineered nanostructures can be employed as active elements in flexible solid‐state ECDs. Indeed, we show that the use of NPs films spray‐coated from water dispersions instead of cast films from chloroform solutions leads to the enhancement of the final ECDs performance in terms of switching time (ST), charge consumption ( Q ), optical contrast (Δ T ), and improved durability (Cycling).…”

Section: Introductionmentioning

confidence: 83%

Processable Thiophene‐Based Polymers with Tailored Electronic Properties and their Application in Solid‐State Electrochromic Devices Using Nanoparticle Films

Moreira

Maria

Zangoli

et al. 2021

Adv Elect Materials

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The development of semiconductor polymers for electronic applications requires tailored synthetic strategies to obtain materials with tunable electronic properties and morphology to enhance their properties. Towards this goal, here is reported the expedient synthesis of a novel class of thiophene‐based electrochromic polymers, processable in organic solvents and as nanoparticles (NPs) in water. Their characterization and application in flexible solid‐state electrochromic devices (ECDs) are described. All polymers have a repeat unit made of the same linear thienyl–phenyl–thienyl–thienyl fragment. The tuning of the electro‐optical properties is achieved by introducing alkyl or alkoxy substituents in thiophene and/or by the presence of either CHCH or CH2CH2 linkers connecting the repeat units and acting as conjugation modulators. The ECDs display a bright yellow or red/magenta color in the neutral state and dark blue in the oxidized state. Redox potentials, color contrast, switching time, and stability of the devices are reported, and it is demonstrated that the use of NPs films spray‐coated from water instead of cast films from chloroform significantly improves their performance. Density functional theory calculations allow to elucidate the relationship between polymer structure and electrochromic properties and shed light on electronic structure changes upon oxidation, in agreement with spectroelectrochemistry.

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“…Cells were illuminated for 10 min with a mercury lamp filtered using a Nikon Texas Red HYQ cubic filter (λ excitation = 532−587 nm, λ emission = 608− 683 nm; power density measured at 550 nm − 70 mW/mm 2 ). After illumination, cells were washed with PBS and incubated for 30 and the fluorescence images were recorded using a Nikon TiS fluorescence microscope. Red fluorescence from propidium iodide and HSA-3a was detected employing a Nikon Texas Red HYQ cubic filter (λ excitation = 532−587 nm, λ emission = 608−683 nm), while using calcein−AM green fluorescence using a Nikon FITC cubic filter (λ excitation = 465−495 nm, λ emission = 515−555 nm).…”

Section: Localized Killing Of Hela Cells By Hsa-3a Bioconjugate By Pr...mentioning

confidence: 99%

Human Serum Albumin–Oligothiophene Bioconjugate: A Phototheranostic Platform for Localized Killing of Cancer Cells by Precise Light Activation

Cantelli

Malferrari

Soldà

et al. 2021

JACS Au

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The electronic, optical, and redox properties of thiophene-based materials have made them pivotal in nanoscience and nanotechnology. However, the exploitation of oligothiophenes in photodynamic therapy is hindered by their intrinsic hydrophobicity that lowers their biocompatibility and availability in water environments. Here, we developed human serum albumin (HSA)–oligothiophene bioconjugates that afford the use of insoluble oligothiophenes in physiological environments. UV–vis and electrophoresis proved the conjugation of the oligothiophene sensitizers to the protein. The bioconjugate is water-soluble and biocompatible, does not have any “dark toxicity”, and preserves HSA in the physiological monomeric form, as confirmed by dynamic light scattering and circular dichroism measurements. In contrast, upon irradiation with ultralow light doses, the bioconjugate efficiently produces reactive oxygen species (ROS) and leads to the complete eradication of cancer cells. Real-time monitoring of the photokilling activity of the HSA–oligothiophene bioconjugate shows that living cells “explode” upon irradiation. Photodependent and dose-dependent apoptosis is one of the primary mechanisms of cell death activated by bioconjugate irradiation. The bioconjugate is a novel theranostic platform able to generate ROS intracellularly and provide imaging through the fluorescence of the oligothiophene. It is also a real-time self-reporting system able to monitor the apoptotic process. The induced phototoxicity is strongly confined to the irradiated region, showing localized killing of cancer cells by precise light activation of the bioconjugate.

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Synthesis, characterization, and biological applications of semiconducting polythiophene‐based nanoparticles

Cited by 26 publications

References 162 publications

Nanoparticle‐Assisted Cation Adduction and Fragmentation of Small Metabolites

Nanoparticle‐Assisted Cation Adduction and Fragmentation of Small Metabolites

Processable Thiophene‐Based Polymers with Tailored Electronic Properties and their Application in Solid‐State Electrochromic Devices Using Nanoparticle Films

Human Serum Albumin–Oligothiophene Bioconjugate: A Phototheranostic Platform for Localized Killing of Cancer Cells by Precise Light Activation

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