Synthesis, characterization and antioxidant activity of silver nanoparticles stabilized by aqueous extracts of wild blackberry (Rubus spp.) and raspberry (Rubus idaeus L.) leaves

Abstract

The green methods of silver nanoparticles (AgNPs) synthesis and their stabilization by biomolecules from aqueous extracts of wild blackberry (Rubus spp.) and raspberry (Rubus idaeus L.) leaves are described. The extracts, obtained from both plants by maceration and reflux extraction were used in nanoparticles synthesis at two different temperatures (room and boiling). Structural characterization of silver nanoparticles synthesized from obtained extracts (AgNPs-E) was performed by UV-Vis and FTIR spectroscopy. The size and high stability of synthesized nanoparticles at room temperature in the dark were proven by the position and monitoring of the surface plasmon resonance (SPR) band in the UV-Vis spectra. A chemical composition of pure extracts and supernatants, obtained by centrifugation of AgNPs-E suspensions, was investigated by LC-MS analysis. Most of the identified compounds belong to phenolic acids and flavonoids derivatives. A comparative LC-MS analysis of the extracts and AgNPs-E supernatants showed that some compounds from the extracts were involved in a higher extent in Ag + ions reduction and formed particles stabilization, suggesting the existence of a structure-activity relationship. The synthesized AgNPs-E showed a comparatively high antioxidant activity estimated by the DPPH test suggesting that they should be taken into account in a further design of skin-protective preparations.

Characterization and Antitumoral Activity of Biohybrids Based on Turmeric and Silver/Silver Chloride Nanoparticles

“…Extracts obtained from plants or waste plants material contain ketones, aldehydes, proteins, flavones, etc., that play a role of reducing and capping agents in the formed AgNPs [ 11 ]. The “green” AgNPs method, in which reducing molecules from extracts binds at the particles’ surface, can be considered successful when a change of the nanoparticles’ suspension color occurs [ 12 ]. Extracts, most often generated from plant leaves of blackberry, raspberry [ 12 ], banana [ 13 ], pepper [ 14 ], Desmodium plant [ 15 ], etc., have been used as a source of phytochemicals for green AgNPs synthesis method, but also from other plant parts, seeds, root, or stem.…”

Comparison of the Micromorphology and Ultrastructure of Pollen Grains of Selected Rubus idaeus L. Cultivars Grown in Commercial Plantation

“…Given the commercial production of its fruit, Rubus idaeus has considerable economic importance in Europe and Poland [ 22 , 23 , 24 , 25 , 26 , 27 ]. Raspberry fruit is intended either for direct consumption or for processing in many industries: food [ 20 , 28 , 29 , 30 , 31 ], pharmaceutical [ 20 , 28 , 32 , 33 ], cosmetic [ 28 , 30 , 33 , 34 , 35 , 36 ], and nanotechnological [ 37 , 38 , 39 , 40 ] industries. Raspberry shrubs are a source of medicinal and cosmetic raw materials, i.e., Rubi idaeus fructus [ 41 , 42 , 43 , 44 , 45 ] and Rubi idaeus folium [ 20 , 46 , 47 , 48 ].…”