The search for effective antibiotics
continues among scientists as more resistant
pathogenic microorganisms evolve, and some
already existing antibiotics used in treatment of
infections are gradually becoming less effective
against the pathogens causing the infections thus,
making life unbearable to their hosts. Phenazines
are para diazine with annular two nitrogen atoms
at 1,4 positions joined to two phenyl group in a side
by side pattern. The derivatives of the compound
are primarily produced from microorganisms and
can also be synthesized, example of this is
phenazine-1-carboxylic acid (PCA). In the present
study, phenazine-1-carboxylic acid was
biosynthesized from Pseudomonas aeruginosa
isolated from soil. Subsequently, phenazineazo-1-
naphthol was synthesized from the produced
phenazine-1-carboxylic acid following amidation
with Ammonium carbonate and Hoffmann
degradation reactions to reduce the compound to
phenazineamine under reflux condition of 50 °C
before diazotization and coupling with 1-naphthol.
The results obtained from the UV-vis, IR and NMR
spectra were able to elucidate the important peaks
in the compound at reasonable extents. The
concentrations of 50 ?g/mL, 100 ?g/mL, 150
?g/mL, 200 ?g/mL, 30 ?g/mL Augmentin (Au,
control drug), 30 ?g/mL Oflocitoxin (OFX, control
drug) and raw sample of the compound were able
to inhibit Staphylococcus aureus and Escherichia
coli at some millimeter ranges while Aspergillus
fumigatus was inhibited by all the sample
concentrations but resisted 30 ?g/mL Augmentin
(Au) and Oflocitoxin (OFX) control drugs from the
antimicrobial analysis carried out. In general, the
compound was active against the organisms when
compared to the concentrations of control
antibiotic drugs used. The minimum inhibitory
concentration of the compound was estimated at 50
?g/mL concentrations.
