Synthesis, Characterization and Antibacterial Activities of Chromium Oxide Nanoparticles Against Klebsiella Pneumoniae

Abstract

Objective: This paper consists of synthesis of chromium oxide (Cr 2 O 3 ) nanoparticles by sol-gel technique, their characterization and investigation of antibacterial activity of these nanoparticles against pathogenic bacteria by measuring zone of inhibition, colony forming units, and optical density (OD) on solid agar media as well as in liquid medium. Methods:The Cr 2 O 3 nanoparticles were synthesized by sol-gel technique using tetraethylorthosilicate as precursor. The synthesized Cr 2 O 3 nanoparticles were characterized by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy, ultraviolet-visible spectroscopy, and transmission electron microscopy (TEM) techniques. The antibacterial effect of these Cr 2 O 3 nanoparticles against Klebsiella pneumoniae was investigated both on the solid agar plates and in liquid medium supplemented with different concentrations of Cr 2 O 3 nanoparticles. The antibacterial activity of Cr 2 O 3 nanoparticles was also compared with the antibacterial activities of the standard antibiotics such as ampicillin, chloramphenicol, penicillin G, streptomycin, sulphatriad, and tetracycline which were taken in the form of hexa discs.Results: Average particle size of the Cr 2 O 3 nanoparticles was found to be 24.0 nm. It was observed that K. pneumoniae is resistant to the penicillin G and ampicillin, but Cr 2 O 3 nanoparticles show good antibacterial property. The minimum inhibitory concentration of Cr 2 O 3 for K. pneumoniae is 2.5 mg/ml. The bacterial growth was monitored by measuring zone of Inhibition, colony formation unit, and OD method. Conclusion:Sol-gel technique is a convenient and easy technique for the synthesis of metal nanoparticles. Nanosized Cr 2 O 3 particles showed an effective antibacterial activity against K. pneumoniae. Therefore, Cr 2 O 3 nanoparticles due to its low manufacturing cost and high effectiveness in antimicrobial properties may find wide applications in various industries to address safety issues.

Antibacterial Function of Chromium Nanoparticles Against K. Pneumonia, E. coli and P. typhus

“…The higher Zone of Inhibition in case of K. pneumonia by the chromium oxide nanoparticles synthesized by sol-gel technique was reported earlier [18] and systematic antimicrobial activity of chromium nanoparticles against pathogenic bacteria also reported earlier (Uma et al 2019 and Kanakalakshmi et al 2017. [3,19]…”

Synthesis, Characterization and Antibacterial Study of Copper Oxide-Graphene Nanocomposites

“…The response of using graphene oxide on microorganisms has been studied extensively. However, the satisfactory results of metal oxide and graphene oxide nanocomposites have not been reported so far [17][18][19][20][21].In continuation to our earlier studies [22][23][24][25][26][27][28], in present study, we have focused on the synthesis of CuO nanoparticles by facile and cost effective sol-gel method and nanocomposite of copper were prepared with reduced graphene oxide. Antibacterial properties of the synthesized nanoparticles and nano- composites were investigated against Gram-positive and Gramnegative bacteria.…”

“…In continuation to our earlier studies [22][23][24][25][26][27][28], in present study, we have focused on the synthesis of CuO nanoparticles by facile and cost effective sol-gel method and nanocomposite of copper were prepared with reduced graphene oxide. Antibacterial properties of the synthesized nanoparticles and nano- composites were investigated against Gram-positive and Gramnegative bacteria.…”

Wet Chemical Synthesis, Characterization, and Antibacterial Activity of Molybdenum Oxide Nanoparticles Against Staphylococcus Epidermidis and Enterobacter Aerogenes

“…Therefore, the ultimate target of nanoscience and nanotechnology is to present opportunities for exploring the bactericidal effect of metal oxide NPs due to their high stability and nontoxic nature [1]. NPs, especially metallic ones, tend to offer an effective solution for overcoming bacterial resistance [2][3][4][5][6][7]. The transition metal oxides are attracting a great deal of attention in the field of material science due to its variety of crystal phases and properties.…”