Synthesis and viscoelastic characterization of microstructurally aligned Silk fibroin sponges

Panda, Debojyoti; Konar, Subhajit; Bajpai, Saumendra; Arockiarajan, A.

doi:10.1016/j.jmbbm.2017.03.029

Cited by 15 publications

(18 citation statements)

References 43 publications

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“…We found that the hindrance was higher in the Z-direction compared to the XY-plane. The effect appeared to be driven by the geometry and design of the surfaces exposed to the freezing planes (Panda et al, 2017).…”

Section: Macroscopic Organizationmentioning

confidence: 99%

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3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

et al. 2020

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Three-dimensional scaffolds play an essential role in tissue engineering. Although essential, the tunability of the 3D scaffolds mechanical and transport properties remains a challenge. In this work, we present new approaches to advance the field. First, we applied our progressive pH acidification to mimic the natural silk gelation process before ice-templating (−20 and −80 • C); second, we fitted the mechanical properties using a connectivity model; third, we fitted the scaffolds mechanical relaxation to understand the transport properties; and fourth we used micro-CT to correlate the process parameters to the scaffolds' performances. Our results suggested that the free shrinkage of the scaffolds determined their final properties. We found, however, that the porosity (above 90%) was anisotropic, similarly the tortuosity (between 1 and 1.3). We identified two major pore dimensions, the first one between 10 and 20 µm, and the second between 50 and 130 µm. Mechanically, our model suggested that the bulk modulus captured the elastic contribution and was controlled predominantly by the silk concentration. We tentatively associated the fractional modulus 1 to the collapse of the larger pores structures and was controlled mostly by the process temperature. We assigned the slow relaxation to the transport of fluid in the silk sponge scaffolds; and the fast relaxation with a viscoelastic relaxation. The silk concentration and process temperatures did not influence the latter. Overall, our use of the tomography, mechanical test, and detailed statistical analysis provides inroads into the interplay between process parameters (silk concentration and process temperature) and the multiple responses of the silk sponge scaffolds. The development of a new mechanical fitting for the compression test helped capture simply the different failure modes in the sponge scaffolds as well as correlating those events to relaxation and eventually transport properties.

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Section: Macroscopic Organizationmentioning

confidence: 99%

“…In 2017, Panda et al obtained RSF sponges with oriented porous channels using a freeze-casting method (Panda et al, 2017). The mechanical properties (experimental and predicted) did not, however, correlate with the microstructure of the sponges (Panda et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

et al. 2020

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“…Silk‐fibroin scaffolds and membranes exhibiting different microstructures were prepared using unidirectional freezing technique as established before (Panda et al, ). During unidirectional cooling/ice‐templating method, the silk‐fibroin protein in the solution get aligned along the direction of cooling.…”

Section: Resultsmentioning

confidence: 99%

“…Silk‐fibroin solution (3% w/v) mixed with acetic acid (1%w/w) was introduced into the device using pipette to achieve a uniform distribution. These devices were then cooled unidirectionally using a previously described setup (Panda, Konar, Bajpai, & Arockiarajan, ). Briefly, the samples were kept in contact with a copper surface and allowed to freeze.…”

Section: Methodsmentioning

confidence: 99%

Collagen‐I/silk‐fibroin biocomposite exhibits microscalar confinement of cells and induces anisotropic morphology and migration of embedded fibroblasts

et al. 2020

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Microstructural anisotropy of tumor-associated matrix correlates with invasion of cancer cells into the surrounding matrix during metastasis. Here, we report the fabrication and characterization of a three-dimensional (3D) silk-fibroin/collagen-I biocomposite based cell-culture model that exhibits microstructural and biochemical anisotropy. Using RGD-deficient silk-fibroin fibers to confine collagen-I gelation, we develop a silk-fibroin/collagen-I (SFC) bio-composite in a one-step process allowing control over the microstructural and biochemical anisotropy and the pore-size. Two forms of the SFC bio-composite are reported: a sandwich (S fc ) configuration amenable to live-cell microscopy and an unsupported membrane (M fc ) for use as a scaffold.Both microscalar and macroscalar mechanical properties of the SFC bio-composite are characterized using atomic force microscope (AFM)-based indentation and tensile-testing. We find that the modulus of stiffness of both S fc and M fc can be controlled and falls in the physiological range of 5-20 kPa. Furthermore, the modulus of stiffness of M fc exhibits a~200% increase in axial direction of microstructure, as compared to lateral direction. This implies a highly anisotropic mechanical stiffness of the microenvironment. Live-cell morphology and migration studies show that both the morphology and the migration of NIH-3 T3 fibroblasts is anisotropic and correlates with microstructural anisotropy. Our results show that SFC bio-composite permits proliferation of cells in both S fc and M fc configuration, promotes cell-migration along the major axis of anisotropy and together with morphological and migration data, suggest a potential application of both the composite configurations as a biomimetic scaffold for tissue engineering applications. K E Y W O R D S aligned scaffold, anisotropy, cell-migration, silk-fibroin SUPPORTING INFORMATION Additional supporting information may be found online in the Supporting Information section at the end of this article. How to cite this article: Konar S, Edwina P, Ramanujam V, Arunachalakasi A, Bajpai SK. Collagen-I/silk-fibroin biocomposite exhibits microscalar confinement of cells and induces anisotropic morphology and migration of embedded fibroblasts.

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“…Among the biological wound dressing materials, silk fibroin (SF) is particularly attractive due to its satisfactory mechanical strength, air permeability, moisture transmission, antibacterial activity, and soft tactile impression . It has been demonstrated that SF membrane can intact with various types of cells to promote their adhesion, proliferation, and growth due to its good biocompatibility and consequently accelerates the healing process of wound . Compared to the SF membrane, SF sponge can be endowed with higher mechanical strength and higher porosity, which has also been proved as suitable wound healing materials .…”

Section: Introductionmentioning

confidence: 99%

Preparation of regenerated silk fibroin‐based heat‐management sponge for wound healing

Liu

Zhao

Wang

et al. 2019

J of Applied Polymer Sci

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In the present study, a regenerated silk fibroin (RSF) sponge incorporated with the phase change microcapsules were synthesized by facile and effective routes. The microcapsules with the phase change materials (PCMs) core and the RSF wall were prepared by simple coacervation. By altering the synthesis parameters including the emulsifier concentration and the RSF concentration, the microcapsules exhibiting optimized spherical geometry, high encapsulation efficiency, high phase change enthalpies, good thermal cycling performance, and high thermal stability were synthesized. As to the preparation of the microstructurally aligned RSF sponge, a new type of freeze–thaw method was used for the first time, the resultant sponge exhibited high elastic resilience. Subsequently, the combination of microcapsules with the RSF sponge was achieved by a convenient deposition method with the aid of ultrasonic agitation, which showed well maintained porous structure and satisfactory phase change enthalpies of 23.16 and 23.32 J•g−1 corresponding to the heating and cooling process, respectively. Therefore, the PCMs‐incorporated RSF sponge exhibited the capacity to regulate the temperature in order to create a stable “microclimate.” Regarding the exceptional biocompatibility, biodegradability, and super comfort of RSF materials, the RSF‐based sponge can be an ideal candidate for wound dressing applications. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 48173.

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Synthesis and viscoelastic characterization of microstructurally aligned Silk fibroin sponges

Cited by 15 publications

References 43 publications

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

3D Structure and Mechanics of Silk Sponge Scaffolds Is Governed by Larger Pore Sizes

Collagen‐I/silk‐fibroin biocomposite exhibits microscalar confinement of cells and induces anisotropic morphology and migration of embedded fibroblasts

Preparation of regenerated silk fibroin‐based heat‐management sponge for wound healing

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