Synthesis and study of antiproliferative, antitopoisomerase II, DNA-intercalating and DNA-damaging activities of arylnaphthalimides

Quintana-Espinoza, Patricia; García-Luis, Jonay; Amesty, Ángel; Martín-Rodríguez, Patricia; Lorenzo-Castrillejo, Isabel; Ravelo, Ángel G.; Fernández-Pérez, Leandro; Machín, Félix; Estévez‐Braun, Ana

doi:10.1016/j.bmc.2013.08.039

Cited by 21 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DNA DSB damage directly induced by BNIPDaCHM is the key component in the toxicity of BNIPDaCHM in MDA-MB-231 cells. This mechanism is also consistent with other novel naphthalimide [35] and bisnaphthalimide derivatives [16,24].…”

Section: Discussionsupporting

confidence: 74%

Bisnaphthalimidopropyl diaminodicyclohexylmethane induces DNA damage and repair instability in triple negative breast cancer cells via p21 expression

Barron

Goua

Kuraoka

et al. 2015

Chemico-Biological Interactions

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 74%

Bisnaphthalimidopropyl diaminodicyclohexylmethane induces DNA damage and repair instability in triple negative breast cancer cells via p21 expression

Barron

Goua

Kuraoka

et al. 2015

Chemico-Biological Interactions

View full text Add to dashboard Cite

“…5 μM, which are also evidenced by the time- and concentration-dependent inhibition of cyclins expression and cleavage of PARP-1 (Figure 5). DNA damage will cause cell cycle arrest and apoptosis, and several naphthalimides have been reported to induce DNA strand breaks both in vitro and in vivo [31, 32]. LSS-11 did not induce cleavage of super coiled plasmid DNA in vitro ; however, it significantly induced DNA fragmentation in cells, as demonstrated by comet assay and flow cytometric analysis (Figure 3D and Figure 6A and Figure 5C).…”

Section: Discussionmentioning

confidence: 92%

A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes

Yang

et al. 2017

Oncotarget

View full text Add to dashboard Cite

DNA and DNA-associated processes have been classes of the most important targets of chemotherapeutic drugs. As classic DNA intercalators and topoisomerase inhibitors, naphthalimides have been extensively investigated as potential anti-cancer drugs. We recently synthesized a novel series of triazolonaphthalimides with excellent anti-cancer activities. In the present study, one of the most potent triazolonaphthalimides, LSS-11, was investigated. LSS-11 bound to DNA in vitro and in cell mainly by minor groove binding and significantly increased the stability of DNA, which could be fundamental for the biological activities of LSS-11. In addition to inhibiting DNA topoisomerase II-catalyzed decatenation of knotted circulated DNA, LSS-11 dramatically inhibited DNA replication mediated by polymerase chain reaction and isothermal helicase-dependent amplification, as well as the expression of luciferase driven by a minimal TA promoter in cell. Furthermore, LSS-11 exhibited strong cytotoxicity in selected human colon cancer cell lines by inducing cell cycle arrest and apoptosis, which was accompanied by DNA damage response. Finally, LSS-11 potently inhibited the growth of S180 murine sarcoma and SW480 human colorectal cancer xenografts in vivo without significant major toxicities. These results suggest that LSS-11 deserves further research and development as a novel anti-cancer agent, and provided new understandings of mechanisms by which LSS-11 inhibited multiple DNA-associated processes and tumor growth.

show abstract

“…A key difference between Top2-interacting drugs resides in whether they just inhibit the reaction or they actually increase the steadystate levels of DSBs, the latter being the mechanism of action of antiTop2 anticancer agents [30]. In order to asses this issue, we performed a cleavage assay, which is carried out with high concentrations of Top2 incubated with the substrate and drug for only a short period of time, stopped prior to the completion of the Top2 reaction [32,46]. We further used the well-known Top2 poison etoposide as a control for this cleavage assay.…”

Section: Yeast Sensitivity To B-lapachone and 3-bromo-b-lapachone Is mentioning

confidence: 99%

“…Anti-topoisomerase II in vitro assays and fluorescence microscopy were performed as previously described [46,47].…”

Section: Other Assaysmentioning

confidence: 99%

Yeast cytotoxic sensitivity to the antitumour agent β-lapachone depends mainly on oxidative stress and is largely independent of microtubule- or topoisomerase-mediated DNA damage

Ramos-Pérez

Lorenzo-Castrillejo

Quevedo

et al. 2014

Biochemical Pharmacology

Self Cite

View full text Add to dashboard Cite

Synthesis and study of antiproliferative, antitopoisomerase II, DNA-intercalating and DNA-damaging activities of arylnaphthalimides

Cited by 21 publications

References 36 publications

Bisnaphthalimidopropyl diaminodicyclohexylmethane induces DNA damage and repair instability in triple negative breast cancer cells via p21 expression

Bisnaphthalimidopropyl diaminodicyclohexylmethane induces DNA damage and repair instability in triple negative breast cancer cells via p21 expression

A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes

Yeast cytotoxic sensitivity to the antitumour agent β-lapachone depends mainly on oxidative stress and is largely independent of microtubule- or topoisomerase-mediated DNA damage

Contact Info

Product

Resources

About