Synthesis and secretion of α‐amylase by rice callus: Evidence for differential gene expression

Simmons, Carl R.; Huang, Ning; Cao, Yiyi; Rodriguez, Raymond L.

doi:10.1002/bit.260380513

Cited by 19 publications

(8 citation statements)

References 24 publications

(1 reference statement)

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“…Mature leaf RNA was isolated as described [50]. Callus m R N A was isolated as previously described [46]. RNA was isolated from panicles 10 d after heading as previously described [20].…”

Section: Rna Isolation and Northern Blottingmentioning

confidence: 99%

Structure of a rice ?-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors

et al. 1992

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A rice beta-glucanase gene was sequenced and its expression analyzed at the level of mRNA accumulation. This gene (Gns1) is expressed at relatively low levels in germinating seeds, shoots, leaves, panicles and callus, but it is expressed at higher levels in roots. Expression in the roots appears to be constitutive. Shoots express Gns1 at much higher levels when treated with ethylene, cytokinin, salicylic acid, and fungal elicitors derived from the pathogen Sclerotium oryzae or from the non-pathogen Saccharomyces cereviseae. Shoots also express Gns1 at higher levels in response to wounding. Expression in the shoots is not significantly affected by auxin, gibberellic acid or abscisic acid. The beta-glucanase shows 82% amino acid similarity to the barley 1,3;1,4-beta-D-glucanases, and from hybridization studies it is the beta-glucanase gene in the rice genome closest to the barley 1,3;1,4-beta-glucanase EI gene. The mature peptide has a calculated molecular mass of 32 kDa. The gene has a large 3145 bp intron in the codon for the 25th amino acid of the signal peptide. The gene exhibits a very strong codon bias of 99% G + C in the third position of the codon in the mature peptide coding region, but only 61% G + C in the signal peptide region.

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“…Mature leaf RNA was isolated as described [50]. Callus m R N A was isolated as previously described [46]. RNA was isolated from panicles 10 d after heading as previously described [20].…”

Section: Rna Isolation and Northern Blottingmentioning

confidence: 99%

Structure of a rice ?-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors

et al. 1992

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“…In wild-type rice cell cultures, it has been demonstrated that an inducible rice R-amylase (RAmy3D) promoter directs high level expression of R-amylase proteins under conditions of low sugar concentrations (15)(16)(17)(18). In addition, a rice R-amylase signal peptide directs secretion of R-amylase into the culture medium.…”

Section: Introductionmentioning

confidence: 99%

Bioreactor Production of Human α₁‐Antitrypsin Using Metabolically Regulated Plant Cell Cultures

Trexler

McDonald

Jackman

2002

Biotechnology Progress

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Transgenic rice cell cultures, capable of producing recombinant human alpha(1)-antitrypsin (rAAT), were scaled up from shake flasks to a 5-L bioreactor. The maximum specific growth rates (mu(max)) observed from two bioreactor runs were 0.40 day(-1) (doubling time of 1.7 days) and 0.47 day(-1) (doubling time of 1.5 days), and the maximum specific oxygen uptake rates were 0.78 and 0.84 mmol O(2)/(g dw h). Using a metabolically regulated rice alpha-amylase (RAmy3D) promoter, signal peptide, and terminator, sugar deprivation turned on rAAT expression, and rAAT was secreted into the culture medium. After 1 day of culture in sugar-free medium, there was still continued biomass growth, oxygen consumption, and viability. Extracellular concentrations of 51 and 40 mg active rAAT/L were reached 1.7 and 2.5 days, respectively, after induction in a sugar-free medium. Volumetric productivities for two batch cultures were 7.3 and 4.6 mg rAAT/(L day), and specific productivities were 3.2 and 1.6 mg rAAT/(g dw day). Several different molecular weight bands of immunoreactive rAAT were observed on immunoblots.

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“…Recombinant human R 1 -antitrypsin (rAAT) was produced by a genetically engineered rice cell, and the expression system used in this work was described in detail in ref 3. An R-amylase promoter, which is induced by sugar depletion, was used in this expression system (9)(10)(11).…”

Section: Methodsmentioning

confidence: 99%

Utilization of an Alternative Carbon Source for Efficient Production of Human α₁‐Antitrypsin by Genetically Engineered Rice Cell Culture

Terashima

Ejiri

Hashikawa

et al. 2001

Biotechnology Progress

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Human alpha(1)-antitrypsin was produced by genetically engineered rice cells using promoter and signal peptide of a rice alpha-amylase isozyme. Batch and continuous cultures were employed to investigate the effects of alternative carbon sources on the alpha(1)-antitrypsin production. While this expression system is inducible by sugar depletion, we have found that the productivity of alpha(1)-antitrypsin increased 2.4- to 3.4-fold, compared with the control medium without carbon source, in medium containing an alternative carbon source, such as pyruvic acid and glyoxylic acid. The accumulated alpha(1)-antitrypsin in the medium containing pyruvic acid reached 18.2-24.2 mg/g-dry cell in 50-70 h by batch culture.

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Synthesis and secretion of α‐amylase by rice callus: Evidence for differential gene expression

Cited by 19 publications

References 24 publications

Structure of a rice ?-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors

Structure of a rice ?-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors

Bioreactor Production of Human α₁‐Antitrypsin Using Metabolically Regulated Plant Cell Cultures

Utilization of an Alternative Carbon Source for Efficient Production of Human α₁‐Antitrypsin by Genetically Engineered Rice Cell Culture

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Synthesis and secretion of α‐amylase by rice callus: Evidence for differential gene expression

Cited by 19 publications

References 24 publications

Structure of a rice ?-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors

Structure of a rice ?-glucanase gene regulated by ethylene, cytokinin, wounding, salicylic acid and fungal elicitors

Bioreactor Production of Human α1‐Antitrypsin Using Metabolically Regulated Plant Cell Cultures

Utilization of an Alternative Carbon Source for Efficient Production of Human α1‐Antitrypsin by Genetically Engineered Rice Cell Culture

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Product

Resources

About

Bioreactor Production of Human α₁‐Antitrypsin Using Metabolically Regulated Plant Cell Cultures

Utilization of an Alternative Carbon Source for Efficient Production of Human α₁‐Antitrypsin by Genetically Engineered Rice Cell Culture