Synthesis and regulation of extracellular β (1–3) glucanase and protease by cytophaga sp. In batch and continuous culture

Abstract

Lytic enzyme systems with the ability to break whole cells of yeast are a mixture of several enzymes and virtually all contain beta(1-3)glucanases and some protease. It appears that the presence of these two enzyme activities is necessary to break the two layers of the rigid cell wall. The enzyme system of Cytophaga NCIB 9497 has a high activity towards the walls of yeast and also of bacteria. This article describes the production of this extracellular lytic enzyme system in batch and continuous culture-it was found to be inducible. The synthesis and regulation of the two main constituent enzymes, beta(1-3)glucanase and protease, have been investigated. The synthesis of beta(1-3)glucanase is regulated by bothinduction (by an unknown inducer) and catabolite repression. Highbeta(1-3)glucanase activities were obtained in continuous culture at low dilution rates over a narrow range (0.05-0.10 h(-1)), and there is evidence of the presence of more than one glucanase enzyme. Proteolytic activity appears subject to catabolite repression and made up of the activities of more than one protease enzyme. Productivity and enzyme concentration were increased several fold in continuous culture when compared to batch culture.

Nature of Escherichia coli cell lysis by culture supernatants of Bacillus species

“…(21,34), and a Cytophaga sp. (1). Previous studies in our laboratory led to the development of practical large-scale applications for using lytic enzymes to recover yeast protein (29).…”

Proteolytic enzymes from Bromelia antiacantha as tools for controlled tissue hydrolysis in entomology

“…Proteolytic activity was determined using azocasein as substrate by a method modified from Andrew and Asenjo (Andrew BA and Asenjo JA, 1986). Briefly, CE was activated for 15 min at 4°C by addition of β-mercaptoethanol to 15 mM.…”

Wool-degrading Bacillus isolates: extracellular protease production for microbial processing of fabrics

“…Culture samples were aseptically removed and cell density at 620 nm was measured to estimate growth. Kinetics of extracellular protease production was done as follows: samples were centrifuged (13,200g for 30 min at 4°C) and the proteolytic activity at 40°C was measured at 340 nm using the cellfree supernatant and Azocasein as substrate (Andrews and Asenjo 1986). One unit of enzyme activity (U) was defined as the amount of cell-free supernatant required to increase one unit of absorbance at 340 nm in the assay conditions.…”