Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging

Tran, Mai; Rarig, Robert-André F.; Chenoweth, David M.

doi:10.1039/c5sc01601k

Cited by 22 publications

(18 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, photoactivatable small molecules are still rare and generally depend on "photoactivation," which refers to the conversion from an initial dark state into a fluorescent state. [2][3][7][8][9] Recently, the Chenoweth group reported a new class of "photoconvertible" dye built upon a 1,1'-diazaxanthilidene scaffold, that utilizes an activation mechanism based on a photochemically allowed 6π electrocyclization/oxidation reaction leading to the conversion of one fluorescent state to another. [8][9][10] Unlike other "photoactivation" mechanisms, such as photoisomerization, 11 photouncaging, 12 or photodecomposition of azides, 13 this "photoconvertible" mechanism facilitates visualization of both the pre-and post-activation forms and without producing potentially harmful side products (e.g.…”

mentioning

confidence: 99%

A “Clickable” Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes

et al. 2019

Self Cite

View full text Add to dashboard Cite

Photoconvertible fluorophores can enable the visualization and tracking of a specific biomolecules, complexes, and cellular compartments with precise spatiotemporal control. The field of photoconvertible probes is dominated by fluorescent protein variants, which can introduce perturbations to the target biomolecules due to their large size. Here, we present a photoconvertible small molecule, termed CPX, that can be conjugated to any target through azidealkyne cycloaddition ("click" reaction). To demonstrate its utility, we have applied CPX to study 1) trafficking of biologically relevant synthetic vesicles and 2) intracellular processes involved in transmission of α-synuclein (αS) pathology. Our results demonstrate that CPX can serve as a minimally perturbing probe for tracking the dynamics of biomolecules.

show abstract

mentioning

confidence: 99%

A “Clickable” Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…To realize this, Chenoweth and coworkers developed photoswitchable lysosome-targeted molecular probe 6 for spatiotemporally imaging the selected cells. [52] Thanks to the [22[ Copyright 2017, the Authors. Published by the Royal Society of Chemistry.…”

Section: Lysosomementioning

confidence: 99%

Section: Lysosomementioning

confidence: 99%

See 1 more Smart Citation

Photoactivatable Biomedical Materials Based on Luminogens with Aggregation‐Induced Emission (AIE) Characteristics

Sun

et al. 2021

Adv Healthcare Materials

View full text Add to dashboard Cite

Fluorescence probes with aggregation-induced emission (AIE) property are fascinating and vital in biological fields due to their bright fluorescence in the solid state. In contrast, traditional AIE materials are obscured by the off-target effects and lack of spatial and temporal control. Photoactivatable materials with AIE characteristics, whose physicochemical behaviors can be remotely activated by light, provide great potential in biochemical information acquisition with high spatial and temporal resolution. By using AIE-featured photoactivatable fluorescence probes, accurate analysis of the targets of interest is possible. For example, where, when, and to what extent a process is started or stopped by manipulating the non-invasive light accurately. Thus, many researchers are enthusiastic about developing AIE-featured photoactivatable materials and mainly focus on developing novel molecules by rational molecular structure design, and exploring advanced applications by appropriate molecular functionalization. In this review, the recent achievements of photoactivatable materials with AIE characteristics from the aspects involving inherent mechanism of photoactivity, molecular design strategy, and the corresponding applications in biological fields, are summarized. The biological applications are highlighted and discussed, including photoactivatable bioimaging, diagnosis, and photo-controlled therapy. Finally, the challenges and prospects of the AIE-featured photoactivatable materials are also outlined and discussed.

show abstract

“…The Hoechst dyes absorb and emit light in the ultraviolet range, and are frequently used in live imaging, for example, to monitor DNA contents during the cell cycle, or viral replication compartments in the cytoplasm [ 62 , 115 , 116 ]. Other dyes that are frequently used in live cell imaging are DRAQ5 to stain DNA in the far-red excitation spectra [ 117 ], the mitochondrial dye chloromethyl-X-rosamine (MitoTracker) [ 118 ] and a series of lysosomotropic dyes for acidic vesicles [ 119 ].…”

Section: Probes and Labeling Strategies For Imaging Of Virusesmentioning

confidence: 99%

Concepts in Light Microscopy of Viruses

Witte

Andriasyan

Georgi

et al. 2018

Viruses

View full text Add to dashboard Cite

Viruses threaten humans, livestock, and plants, and are difficult to combat. Imaging of viruses by light microscopy is key to uncover the nature of known and emerging viruses in the quest for finding new ways to treat viral disease and deepening the understanding of virus–host interactions. Here, we provide an overview of recent technology for imaging cells and viruses by light microscopy, in particular fluorescence microscopy in static and live-cell modes. The review lays out guidelines for how novel fluorescent chemical probes and proteins can be used in light microscopy to illuminate cells, and how they can be used to study virus infections. We discuss advantages and opportunities of confocal and multi-photon microscopy, selective plane illumination microscopy, and super-resolution microscopy. We emphasize the prevalent concepts in image processing and data analyses, and provide an outlook into label-free digital holographic microscopy for virus research.

show abstract

Synthesis and properties of lysosome-specific photoactivatable probes for live-cell imaging

Abstract: We describe the synthesis and application of a new class of large Stokes shift lysosome-specific photoactivatable probes for live-cell imaging.

Cited by 22 publications

References 18 publications

A “Clickable” Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes

A “Clickable” Photoconvertible Small Fluorescent Molecule as a Minimalist Probe for Tracking Individual Biomolecule Complexes

Photoactivatable Biomedical Materials Based on Luminogens with Aggregation‐Induced Emission (AIE) Characteristics

Concepts in Light Microscopy of Viruses

Contact Info

Product

Resources

About