Synthesis and properties of calcium hydroxyapatite/silk fibroin organomineral composites

Zakharov, N. A.; Демина, Л. И.; Алиев, А. Д.; Киселев, М. Р.; Матвеев, В. В.; Orlov, M. A.; Zakharova, T. V.; Кузнецов, Н. Т.

doi:10.1134/s0020168517030128

Cited by 16 publications

(9 citation statements)

References 38 publications

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“…Hydrogels, which contained brushite, showed faster degradation and lower insoluble mass percent, contrarily samples with HAp were more stable and had higher gel fractions. Overall gel fraction is higher if Ca 2+ ions are included in the hydrogel because Ca 2+ ions make the polymer matrix more stable by creating an electrostatic interaction with silk and gelatin [ 43 ]. The polar and charged side chain groups inside the silk structure work as nucleation centers for CaP [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Enzymatically Crosslinked In Situ Synthesized Silk/Gelatin/Calcium Phosphate Hydrogels for Drug Delivery

2021

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Our research focuses on combining the valuable properties of silk fibroin (SF) and calcium phosphate (CaP). SF is a natural protein with an easily modifiable structure; CaP is a mineral found in the human body. Most of the new age biocomposites lack interaction between organic/inorganic phase, thus SF/CaP composite could not only mimic the natural bone, but could also be used to make drug delivery systems as well, which can ensure both healing and regeneration. CaP was synthesized in situ in SF at different pH values, and then crosslinked with gelatin (G), horseradish peroxide (HRP), and hydrogen peroxide (H2O2). In addition, dexamethasone phosphate (DEX) was incorporated in the hydrogel and drug delivery kinetics was studied. Hydrogel made at pH 10.0 was found to have the highest gel fraction 110.24%, swelling degree 956.32%, and sustained drug delivery for 72 h. The highest cell viability was observed for the hydrogel, which contained brushite (pH 6)—512.43%.

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Section: Discussionmentioning

confidence: 99%

Enzymatically Crosslinked In Situ Synthesized Silk/Gelatin/Calcium Phosphate Hydrogels for Drug Delivery

2021

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“…Furthermore, preferential growth of the crystals is observed since the c -axis (002) is aligned with the long-axis direction of fibroin. These events suggest that fibroin acts as a template that provides active sites for the nucleation and growth of HAp. ,,− On the other hand, fibroin does not influence the crystallinity of HAp, being that HAp/Fibroin composites are characterized by a low crystallinity. ,,,− These biomaterials have also demonstrated to have better biological properties in vitro than pure HAp in human osteoblast-like cell (MG-63) adhesion and proliferation. , In vivo studies using New Zealand adult rabbits confirmed these behaviors . When the percentage of silk fibroin incorporated into the HAp/Fibroin composite is low (2 wt %), the material is fragile and brittle (Table ).…”

Section: Fibroinmentioning

confidence: 99%

Protein-Based Hydroxyapatite Materials: Tuning Composition toward Biomedical Applications

Veiga

Castro

Rocha

et al. 2020

ACS Appl. Bio Mater.

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Synthetic hydroxyapatite (HAp) has been successfully produced with the aim of obtaining biomaterials that meet the biomechanical requirements for bone tissue engineering, while being compatible with the surrounding biochemical and cellular environment. Combining proteins with HAp can generate improved composite biomaterials, which are environmentally friendly, renewable, and biocompatible. In this context, HAp/protein-based composite materials have been widely exploited since the late 20th century to the present day. In this review, we explore the biomedical relevance of the association of HAp with several proteins of interest such as fibroin, sericin, fibrin, and keratin. The processing strategies for their synthesis and effect on the obtained shape and physicochemical, mechanical, and biological performance are herein discussed. This work can provide useful information for the design of HAp-based biomaterials with specific emphasis on bone tissue regeneration characteristics for biomedical applications.

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“…Because of their osteoconductive properties, silk materials containing HAP, bioactive ceramics and silica NPs have been studied. Nanocomposite scaffolds of silk and HAP are particularly interesting due to the ability of silk to “regulate” the mineralization of calcium phosphates, presumably through chemical interactions involving silk chemical groups (Kong et al, 2004 ; Zakharov et al, 2017 ). Silk/HAP scaffolds were manufactured using thermally induced phase separation (TIPS) (Wei and Ma, 2004 ) or lyophilization (Ye et al, 2017 ), to generate highly porous silk/nHAP composite scaffolds.…”

Section: Silk-based Bionanocompositesmentioning

confidence: 99%

Silk Polymers and Nanoparticles: A Powerful Combination for the Design of Versatile Biomaterials

et al. 2020

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Silk fibroin (SF) is a natural protein largely used in the textile industry but also in biomedicine, catalysis, and other materials applications. SF is biocompatible, biodegradable, and possesses high tensile strength. Moreover, it is a versatile compound that can be formed into different materials at the macro, micro- and nano-scales, such as nanofibers, nanoparticles, hydrogels, microspheres, and other formats. Silk can be further integrated into emerging and promising additive manufacturing techniques like bioprinting, stereolithography or digital light processing 3D printing. As such, the development of methodologies for the functionalization of silk materials provide added value. Inorganic nanoparticles (INPs) have interesting and unexpected properties differing from bulk materials. These properties include better catalysis efficiency (better surface/volume ratio and consequently decreased quantify of catalyst), antibacterial activity, fluorescence properties, and UV-radiation protection or superparamagnetic behavior depending on the metal used. Given the promising results and performance of INPs, their use in many different procedures has been growing. Therefore, combining the useful properties of silk fibroin materials with those from INPs is increasingly relevant in many applications. Two main methodologies have been used in the literature to form silk-based bionanocomposites: in situ synthesis of INPs in silk materials, or the addition of preformed INPs to silk materials. This work presents an overview of current silk nanocomposites developed by these two main methodologies. An evaluation of overall INP characteristics and their distribution within the material is presented for each approach. Finally, an outlook is provided about the potential applications of these resultant nanocomposite materials.

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Synthesis and properties of calcium hydroxyapatite/silk fibroin organomineral composites

Cited by 16 publications

References 38 publications

Enzymatically Crosslinked In Situ Synthesized Silk/Gelatin/Calcium Phosphate Hydrogels for Drug Delivery

Enzymatically Crosslinked In Situ Synthesized Silk/Gelatin/Calcium Phosphate Hydrogels for Drug Delivery

Protein-Based Hydroxyapatite Materials: Tuning Composition toward Biomedical Applications

Silk Polymers and Nanoparticles: A Powerful Combination for the Design of Versatile Biomaterials

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