Synthesis and properties of amphiphilic networks. 1: the effect of hydration and polymer composition on the adhesion of immunoglobulin-G to poly(laurylmethacrylate-stat-glycerolmonomethacrylate-stat-ethylene-glycol-dimethacrylate) networks

Haigh, Robert; Rimmer, Stephen; Fullwood, Nigel J.

doi:10.1016/s0142-9612(99)00245-8

Cited by 52 publications

(49 citation statements)

References 25 publications

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“…The fibroblast feeder layer also reduces the degree of differentiation of keratinocytes, which may be advantageous for initial wound healing, and the fibroblasts can be derived from several sources -murine, human, donor or autologous cells [25]. Similarly with respect to the cornea we recently confirmed that corneal fibroblasts will support freshly isolated human corneal epithelial cells in 2D co-culture on a range of surfaces produced by plasma polymerization under serum free conditions [29].…”

Section: Discussionmentioning

confidence: 66%

“…In this paper we describe how we have combined amine modification of the surface of a hydrogel with a knowledge of epithelial/stromal cell dependency (as detailed in [24][25][26][27][28] to control and optimise epithelialization on hydrogel surfaces. In brief our prior experience with stromal cell/epithelial cell co-culture has shown that it is possible to achieve epithelial cell growth (both primary human keratinocytes [25][26][27][28] and primary human corneal epithelial cells) [29] on a range of substrates providing one co-cultures them with stromal cells. Cell culture conditions can be achieved where stromal cells act as initial support/feeder cell for epithelial cells so that epithelial cells can be grown serum-free on substrates where they would not previously grow unaided [25][26][27][28][29].…”

Section: Epithelialization Of Hydrogels Achieved By Amine Functionalimentioning

confidence: 99%

“…Figure 1 shows the series of amines that were attached to the glycidyl ether groups of the terpolymer and the resultant functionality. The equilibrium water contents were measured gravimetrically as described previously [29] and are shown in Figure 3. The data show that the structure of the amine does not have much effect on equilibrium swelling and, therefore, this aspect is not a major factor in the EWC attained by these hydrogels.…”

Section: Preparation Of Amine Modified Hydrogelsmentioning

confidence: 99%

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Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

et al. 2007

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(2007). Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells. Biomaterials, 28 (35), 5319 -5331. The aim of this study was to develop a hydrogel which would be suitable for corneal cell re-epithelialization when used as a corneal implant. To achieve this, a series of hydrogels were functionalized with primary amines by post-polymerization reactions between amine compounds and glycidyl ether groups attached to the hydrogels. We report a strong correlation between the structure of the amine and the viability of stromal cells and epithelial cells cultured on these hydrogels. Subsequent co-culture of epithelial and stromal cells on the amine modified hydrogels allowed successful expansion of epithelial cells on surfaces functionalised with alkyl α-ω diamines with carbon chain lengths of between 3 and 6. Analysis of variance showed that corneal epithelial cells had a strong preference for surfaces functionalized by the reaction of excess 1,3 diaminopropane with units of glycidyl methacrylate compared to the reaction products of other amines (ammonia; 1,2-diaminoethane; 1,4-diaminobutane or 1,6-diaminohexane). We suggest this approach of amine functionalization combined with stromal/epithelial co-culture offers a promising new approach to achieving a secure corneal epithelium. Copyright and re-use policy AUTHOR DECLARATIONWe the undersigned declare that this manuscript is original, has not been published before and is not currently being considered for publication elsewhere.We wish to confirm that there are no known conflicts of interest associated with this publication and there has been no significant financial support for this work that could have influenced its outcome.We confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us.We confirm that we have given due consideration to the protection of intellectual property associated with this work and that there are no impediments to publication, including the timing of publication, with respect to intellectual property. In so doing we confirm that we have followed the regulations of our institutions concerning intellectual property.We further confirm that any aspect of the work covered in this manuscript that has involved either experimental animals or human patients has been conducted with the ethical approval of all relevant bodies and that such approvals are acknowledged within the manuscript.We understand that the Corresponding Author is the sole contact for the Editorial process (including Editorial Manager and direct communications with the office). He is responsible for communicating with the other authors about progress, submissions of revisions and final approval of proofs. We confirm that we have provided a current, correct email address which is accessible by the Corresponding Author and which ...

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Section: Discussionmentioning

confidence: 66%

Section: Epithelialization Of Hydrogels Achieved By Amine Functionalimentioning

confidence: 99%

Section: Preparation Of Amine Modified Hydrogelsmentioning

confidence: 99%

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Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

et al. 2007

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“…Poly(glycerol monomethacrylate) (PGMA) is a hydrophilic polymer that has been shown to be biocompatible. 61 GMA is a speciality monomer available from Cognis that is prepared via acetone protection of glycerol, followed by esterification of the remaining alcohol. One consequence of this preparation route is that GMA exists in two isomeric forms (see Figure 1.13).…”

Section: Atom Transfer Radical Polymerisationmentioning

confidence: 99%

Covalently Cross-Linked Colloidosomes

et al. 2010

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Model poly(glycerol monomethacrylate)-based macromonomers have been used to prepare sterically stabilized polystyrene latexes by either aqueous emulsion or alcoholic dispersion polymerization, affording mean latex diameters of approximately 107 or 1188 nm respectively as judged by dynamic light scattering. Such PGMA50−PS latexes have appropriate surface wettability to stabilize 25−250 μm oil-in-water Pickering emulsions, depending on the latex concentration and oil type. Colloidosomes were formed by covalent cross-linking of the hydroxyl-functional stabilizer chains from within the oil droplets using a polymeric diisocyanate (tolylene 2,4-diisocyanate-terminated poly(propylene glycol) [PPG-TDI]. This oil-soluble cross-linker was confined within the oil droplets, allowing colloidosomes to be prepared at 50 vol % solids without any aggregation. The resulting microcapsules survive removal of the internal oil phase using excess alcohol, unlike the noncross-linked Pickering emulsion precursor. These observations confirm the robust nature of these covalently stabilized colloidosomes. A method for controlling the permeability of these colloidosomes by exploiting the solvent properties of binary oil mixtures has also been evaluated. Finally, microcapsule permeability has been explored via dye release experiments. Less permeable microcapsules can be obtained by deposition of polypyrrole onto the colloidosome exterior.

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“…The capability of producing scaffolds without the use of any toxic solvent makes this technique unique and extremely interesting with respect to all other scaffold fabrication methods known to date. However, it is well documented that foamed scaffolds can exhibit inadequate pore interconnectivity especially at the scaffold surface, where a non-porous layer forms probably because of the rapid diffusion of the CO 2 from the surface as the pressure is released [123]. This issue has been overcome by combining gas foaming with the salt leaching procedure [117,124] or by trimming off the non-porous skin from the scaffold after its fabrication [125,126].…”

Section: Gas Foamingmentioning

confidence: 99%

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

Gualandi

2011

Springer Theses

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and some lactide copolymers) and of non-commercial polyesters (i.e. poly-ω-pentadecalactone and some of its copolymers) were explored and discussed.Two techniques were employed to fabricate scaffolds: supercritical carbon dioxide (scCO 2 ) foaming and electrospinning (ES). The former is a powerful technology that enables to produce 3D microporous foams by avoiding the use of solvents that can be toxic to mammalian cells. The scCO 2 process, which is II commonly applied to amorphous polymers, was successfully modified to foam a highly crystalline poly(ω-pentadecalactone-co-ε-caprolactone) copolymer and the effect of process parameters on scaffold morphology and thermo-mechanical properties was investigated.In the course of the present research activity, sub-micrometric fibrous nonwoven meshes were produced using ES technology. Electrospun materials are considered highly promising scaffolds because they resemble the 3D organization of native extra cellular matrix. A careful control of process parameters allowed to fabricate defect-free fibres with diameters ranging from hundreds of nanometers to several microns, having either smooth or porous surface. Moreover, versatility of ES technology enabled to produce electrospun scaffolds from different polyesters as well as "composite" non-woven meshes by concomitantly electrospinning different fibres in terms of both fibre morphology and polymer material. The 3D-architecture of the electrospun scaffolds fabricated in this research was controlled in terms of mutual fibre orientation by properly modifying the instrumental apparatus. This aspect is particularly interesting since the micro/nano-architecture of the scaffold is known to affect cell behaviour.Since last generation scaffolds are expected to induce specific cell response, the present research activity also explored the possibility to produce electrospun scaffolds bioactive towards cells. Bio-functionalized substrates were obtained by loading polymer fibres with growth factors (i.e. biomolecules that elicit specific cell behaviour) and it was demonstrated that, despite the high voltages applied during electrospinning, the growth factor retains its biological activity once

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Synthesis and properties of amphiphilic networks. 1: the effect of hydration and polymer composition on the adhesion of immunoglobulin-G to poly(laurylmethacrylate-stat-glycerolmonomethacrylate-stat-ethylene-glycol-dimethacrylate) networks

Cited by 52 publications

References 25 publications

Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

Epithelialization of hydrogels achieved by amine functionalization and co-culture with stromal cells

Covalently Cross-Linked Colloidosomes

Porous Polymeric Bioresorbable Scaffolds for Tissue Engineering

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