Synthesis and properties of a potential extracellular fluorescent probe for potassium

Abstract

Development of a new, selective, high affinity, fluorescent probe CD222.2, for potassium is described.Coincidentally two related probes, CDFI 8,3, and CTFI 8,4, were prepared and showed selectivity for the cations sodium and calcium, respectively, and possible reasons for these differences are discussed. The bicyclic probe CD222.2, with observed K, values for K + ranging from 1 to 10 mmol dmP3, has potential applications as an extracellular probe for potassium.

G Quadruplex‐Based FRET Probes with the Thrombin‐Binding Aptamer (TBA) Sequence Designed for the Efficient Fluorometric Detection of the Potassium Ion

“…Interestingly, even in the FAT-7 probe, which contains a relatively long spacer that should protect fluorophores from direct interactions, non-FRET quenching operates (the calculated R = 48 cannot reflect the actual separation distance). An alternative explanation for the peculiar FRET results for FAT-n probes in the presence of KCl may involve other factors connected with the proper parameterization of Equation (2). For example k 2 may take a value between 0 and 4 for DNA-attached fluorophores, [9,32] variations in fluorophore spectra influence the spectral overlap J(n) [11] and finally, the critical radius R o may be different in the case of folded and unfolded probes.…”

“…[1,2] Recently, selective and sensitive K + assays based on quadruplexforming oligonucleotides have been reported. [3,4] Guanine-rich oligonucleotides are known to form G quadruplex structures.…”

1H and 13C NMR assignments and X‐ray structures for three monocyclic benzoannelated dilactam polyethers

“…Monocyclic dilactams 1, 2 and 3 were synthesized using the procedures described by Aguilar et al, 13 Crossley et al 15 and Dietrich et al 16 The target compounds were purified by crystallization from ethanol (1, 2) and chloroform ( 3). …”

An Aptamer-based and Pyrene-labeled Fluorescent Biosensor for Homogeneous Detection of Potassium Ions

“…However, most of the reported studies concerning K + avoided their clinical applications. For example, Minta et al 2 and Crossley et al 3 had reported K + detection in biological fluids that were based on fluorophores PBFI and coumarin diacid cryptand [2.2.2], respectively. However, poor selectivity against Na + was shown because they only tolerated 1.5-and 3.4-fold excesses of Na + in biological fluids, respectively.…”