Synthesis and production of poly(3-hydroxyvaleric acid) homopolyester by Chromobacterium violaceum

Steinbüchel, Alexander; Debzi, E. M.; Marchessault, R. H.; Timm, Arnulf

doi:10.1007/bf00205030

Cited by 108 publications

(68 citation statements)

References 27 publications

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“…The homopolyesters PHB and PHV were isolated from sodium gluconate-grown cells of A. eutrophus H16 and sodium valerate-grown cells of Chromobacterium violaceum (51), respectively, by the sodium hypochlorite method as described previously (26,36). PHO was isolated from Pseudomonas oleovorans grown in mineral medium with sodium octanoate (0.75%, wt/vol) as the carbon source, and poly(3-hydroxybutyrate-co-3-hydroxydecanoate) [P(HO-co-HD)] was isolated from sodium gluconategrown cells (1.5%, wt/vol) of P. putida KT2440, as described by Timm and Steinbüchel (56).…”

Section: Methodsmentioning

confidence: 99%

Biochemical and molecular characterization of the Pseudomonas lemoignei polyhydroxyalkanoate depolymerase system

et al. 1995

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Two types of C-terminal sequences apparently represent substrate-binding sites; the PHB type is present in the PHB depolymerases of A. faecalis and P. pickettii and in PhaZ2, PhaZ3, and PhaZ5, and the PHV type is present in the PHV-hydrolyzing depolymerases (PhaZ4 and PhaZ1). phaZ1 was transferred to A. eutrophus H16 and JMP222. All transconjugants of both strains were able to grow with extracellular PHB as a carbon source and produced translucent halos on PHB-containing solid media. PhaZ1, PhaZ2, PhaZ4, and PhaZ5 were purified from P. lemoignei and from recombinant E. coli; the processing sites of the precursors in E. coli were the same as in P. lemoignei, and similar substrate specificities were determined for the wild-type and the recombinant proteins. All PHA depolymerases hydrolyzed PHB at high specific activities. PhaZ1 and PhaZ4 additionally cleaved PHV, and PhaZ4 hydrolyzed poly(4-hydroxybutyrate). None of the depolymerases was able to hydrolyze polylactide or PHA consisting of monomers with more than five carbon atoms. While the wild-type depolymerase proteins were glycosylated and found to contain glucose and N-acetylglucosamine, none of the recombinant proteins was glycosylated. PHB hydrolysis was dependent on divalent cations such as Ca 2؉ and was inhibited by the presence of EDTA.

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Section: Methodsmentioning

confidence: 99%

Biochemical and molecular characterization of the Pseudomonas lemoignei polyhydroxyalkanoate depolymerase system

et al. 1995

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“…Polyhydroxybutyrate (PHB), an scl-PHA, is found in many bacterial cells and it is known to be a brittle thermoplastic. The nature and proportion of different monomers are infl uenced by the bacterial strains, type and relative quantity and quality of carbon sources supplied to the growth medium [1]. Increase in hydroxyvalerate (HV) content in the copolymer containing poly(hydroxybutyrate-co-hydroxyvalerate)-P(HB-co-HV) leads to improved mechanical property such as fl exibility and strength [2].…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of bacterial polyhydroxyalkanoate copolymers and evaluation of their blends by fourier transform infrared spectroscopy and scanning electron microscopy

et al. 2009

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Rhizobium meliloti produced a copolymer of short chain length polyhydroxyalkanoate (scl-PHA) on sucrose and rice bran oil as carbon substrates. Recombinant Escherichia coli (JC7623ABC1J4), bearing PHA synthesis genes, was used to synthesize short chain length-co-medium chain length PHA (scl-co-mcl-PHA) on glucose and decanoic acid. Fourier transform infrared spectroscopy (FTIR) spectra of the PHAs indicated strong characteristic bands at 1282, 1723, and 2934 cm -1 for scl-PHA and at 2933 and 2976 cm -1 for scl-co-mcl-PHA polymer. Differentiation of polyhydroxybutyrate (PHB) and polyhydroxybutyrate-co-hydroxyvalerate-P(HB-co-HV) copolymer was obseverd using FTIR, with absorption bands at 1723 and 1281 for PHB, and at 1738, 1134, 1215 cm -1 for HV-copolymer. The copolymers were analyzed by GC and 1 H NMR spectroscopy. Films of polymer blends of PHA produced by R. meliloti and recombinant E. coli were prepared using glycerol, polyethylene glycol, polyvinyl acetate, individually (1:1 ratio), to modify the mechanical properties of the fi lms and these fi lms were evaluated by FTIR and scanning electron microscopy.

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“…Among the PHAs, poly(β-hydroxybutyrates) (3-hydroxybutyric acid) is the first discovered homopolymer, with wide varieties of microbes accumulated and has most similar properties to that of polypropylene. Sometimes few reports are related to non-PHB homopolymers, including P4HB [157,158], poly(3-hydroxypropionate) (P3HP) [159], poly(3-hydroxy-5-phenylvaleric acid) [47], poly(3-hydroxyvalerate) (PHV) [160], poly(3-hydroxyhexanoate) (PHHx) [47,133], poly(3-hydroxyoctanoate) (PHO) [161], poly(3-hydroxyheptanoate) (PHHp) [161], poly(3-hydroxydodecanoate) (PHDD) [162], poly(3-hydroxydodecanoate) (PHD), and poly(3-hydroxynonanoate) (PHN) [163]. …”

Section: Properties Of Phasmentioning

confidence: 99%

Exploring biodegradable polymer production from marine microbes

Rasu¹,

Arun²

2017

Biodegradable Polymers: Recent Developments and New Perspectives

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Synthesis and production of poly(3-hydroxyvaleric acid) homopolyester by Chromobacterium violaceum

Cited by 108 publications

References 27 publications

Biochemical and molecular characterization of the Pseudomonas lemoignei polyhydroxyalkanoate depolymerase system

Biochemical and molecular characterization of the Pseudomonas lemoignei polyhydroxyalkanoate depolymerase system

Production and characterization of bacterial polyhydroxyalkanoate copolymers and evaluation of their blends by fourier transform infrared spectroscopy and scanning electron microscopy

Exploring biodegradable polymer production from marine microbes

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