Synthesis and ITC characterization of novel nanoparticles constituted by poly(γ‐benzyl L‐glutamate)‐β‐cyclodextrin

Abstract

Imparting desired technological characteristics to polymeric nanoparticles requires the development of original polymers. In the present work, the synthesis and characterization of a novel PBLG-derivative, the poly(gamma-benzyl L-glutamate)-beta-cyclodextrin (PBLG-beta-CD-50), have been carried out. Nanoparticles from either PBLG-beta-CD-50 polymer or from mixtures with PBLG have been prepared using a modified nanoprecipitation method. Spherically shaped nanoparticles with diameter in the range of 50-70 nm were obtained, as determined by dynamic laser light scattering and transmission electron microscopy. The presence of a surfactant in the suspension medium had almost no influence on these parameters and was not necessary to the shelf-stability of the suspension. Further, isothermal titration microcalorimetry (ITC) experiments have been used to show unambiguously that about 20% of the cyclodextrins remain functional within the particles. Consequently, this system may be of interest when association of large amounts of hydrophobic drugs to nanoparticles is required.

Methods for the Preparation and Manufacture of Polymeric Nanoparticles

“…To use cyclodextrins as anchor point of a ligand on the nanoparticle surface, the small hydrophobic molecule making inclusion complex with the cyclodextrin is grafted on the ligand (187,197). In the case the anchorage platform is composed by a biotin residue, the ligand is attached on the nanoparticle surface via avidinbiotin complex formation (188,198).…”

Gold Nanoparticles Functionalized with Deep-Cavity Cavitands: Synthesis, Characterization, and Photophysical Studies

“…The complexation behavior was further probed by isothermal titration calorimetry measurements. − Thermodynamic parameters for the complexation of 7 , 8 , 10 , and 11 with TT and TT∩AuNP were determined by monitoring the heat changes during the titration of the guest into a host solution. (For representative ITC data, see Figures S26–S33 in the SI.)…”

Obtaining nonspherical poly(alkylcyanoacrylate) nanoparticles by the stretching method applied with a marketed water-soluble film

“…This method was used to produce nanocubes and nanorods. Alternatively, nanoparticles with an elongated shape could be generated by the method of nanoprecipitation which is widely used to produce spherical nanoparticles from polymers [6,24]. However, it requires the use of specifically conformed polymers, including a few polypeptides, able to self-associate together to form crystalline domains that are believed to give an elongated shape to nanoparticles [24].…”

“…Alternatively, nanoparticles with an elongated shape could be generated by the method of nanoprecipitation which is widely used to produce spherical nanoparticles from polymers [6,24]. However, it requires the use of specifically conformed polymers, including a few polypeptides, able to self-associate together to form crystalline domains that are believed to give an elongated shape to nanoparticles [24]. Alternatively, elongated nanoparticles were also produced by stretching spherical nanoparticles, which can be previously prepared by a suitable method [4,[6][7][8].…”