Synthesis and interaction with human serum albumin of the first 3,18-disubstituted derivative of bilirubin

The role of polymer size and hydrophobic end-group in PEG–protein interaction

“…The protein concentration was determined spectrophotometrically using the extinction coefficient of 35,219 M −1 cm −1 at 280 nm [19]. PEG-3000, PEG-6000 and mPEG-anthracene (0.25 mM) were dissolved in Tris-HCl solution (pH 7.4) and diluted to different concentrations.…”

Folic acid complexes with human and bovine serum albumins

“…The protein concentration was determined spectrophotometrically using the extinction coefficient of 36,500 M À1 cm À1 at 280 nm (Painter, Harding, & Beeby, 1998). A folic acid solution of 1 mM was prepared in 10 mM Tris-HCl and diluted to various concentrations in Tris-HCl (pH 7.4).…”

An overview of protein-DNA and protein-RNA interactions

“…Human serum albumin (Structure 1) is a principal extracellular protein with a high concentration in blood plasma (40 mg ml -1 ) [15][16][17][18]. HSA is a globular protein composed of three structurally similar domains (I, II, and III), each containing two subdomains (A and B) and stabilized by 17 disulphide bridges [19][20][21][22]. Aromatic and heterocyclic ligands were found to bind within two hydrophobic pockets in subdomains IIA and IIIA, namely site I and site II [19][20][21][22].…”

“…HSA is a globular protein composed of three structurally similar domains (I, II, and III), each containing two subdomains (A and B) and stabilized by 17 disulphide bridges [19][20][21][22]. Aromatic and heterocyclic ligands were found to bind within two hydrophobic pockets in subdomains IIA and IIIA, namely site I and site II [19][20][21][22]. Seven binding sites are localized for fatty acids in subdomains IB, IIIA, IIIB, and on the subdomain interfaces [16].…”

“…HSA has also high affinity metal binding site at the N-terminus [15]. The multiple binding sites underlie the exceptional ability of HSA to interact with many organic and inorganic molecules and make this protein an important regulator of intercellular fluxes, as well as the pharmacokinetic behavior of many drugs [15][16][17][18][19][20][21][22].…”