Synthesis and in vitro evaluation of fluorescent and magnetic nanoparticles functionalized with a cell penetrating peptide for cancer theranosis

Perillo, E.; Hervé-Aubert, Katel; Allard-Vannier, Émilie; Falanga, Annarita; Galdiero, Stefania; Chourpa, Igor

doi:10.1016/j.jcis.2017.03.106

Cited by 51 publications

(35 citation statements)

References 27 publications

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“…Others groups conclude that the functionalization of SPIONs with scFv do not significantly increase their diameter as scFv are relatively small entities of approximately 5 nm [ 20 ]. As noted previously [ 21 ], the presence of Cy dye was confirmed by the strong fluorescence signal recorded from the functionalized SPIONs suspended in water (Additional file 1 : Fig. S2B).…”

Section: Resultssupporting

confidence: 81%

Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes

et al. 2018

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BackgroundRecent advances in nanomedicine have shown the great interest of active targeting associated to nanoparticles. Single chain variable fragments (scFv) of disease-specific antibodies are very promising targeting entities because they are small, not immunogenic and able to bind their specific antigens. The present paper is devoted to biological properties in vitro and in vivo of fluorescent and pegylated iron oxide nanoparticles (SPIONs-Cy-PEG-scFv) functionalized with scFv targeting Human Epithelial growth Receptor 2 (HER2).ResultsThanks to a site-selective scFv conjugation, the resultant nanoprobes demonstrated high affinity and specific binding to HER2 breast cancer cells. The cellular uptake of SPIONs-Cy-PEG-scFv was threefold higher than that for untargeted PEGylated iron oxide nanoparticles (SPIONs-Cy-PEG) and is correlated to the expression of HER2 on cells. In vivo, the decrease of MR signals in HER2+ xenograft tumor is about 30% at 24 h after the injection.ConclusionsThese results all indicate that SPIONs-Cy-PEG-scFv are relevant tumor-targeting magnetic resonance imaging agents, suitable for diagnosis of HER2 overexpressing breast tumor.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0341-6) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 81%

Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes

et al. 2018

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“…Note that though gH625-functionalized iron nanoparticles have recently been prepared by Perillo et al [ 24 ], in the present work, we report a different synthetic strategy based on a highly versatile phosphonic acid platform. In particular, phosphonic acid chemistry allows the formation of strong bonds between MNPs and phosphonic monolayers whose stability is comparable to that of silanized MNP.…”

Section: Introductionmentioning

confidence: 93%

Comparison Between Folic Acid and gH625 Peptide-Based Functionalization of Fe3O4 Magnetic Nanoparticles for Enhanced Cell Internalization

et al. 2018

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A versatile synthetic route based on magnetic Fe3O4 nanoparticle (MNP) prefunctionalization with a phosphonic acid monolayer has been used to covalently bind the gH625 peptide on the nanoparticle surface. gH625 is a membranotropic peptide capable of easily crossing the membranes of various cells including the typical human blood-brain barrier components. A similar synthetic route was used to prepare another class of MNPs having a functional coating based on PEG, rhodamine, and folic acid, a well-known target molecule, to compare the performance of the two cell-penetrating systems (i.e., gH625 and folic acid). Our results demonstrate that the uptake of gH625-decorated MNPs in immortalized human brain microvascular endothelial cells after 24 h is more evident compared to folic acid-functionalized MNPs as evidenced by confocal laser scanning microscopy. On the other hand, both functionalized systems proved capable of being internalized in a brain tumor cell line (i.e., glioblastoma A-172). These findings indicate that the functionalization of MNPs with gH625 improves their endothelial cell internalization, suggesting a viable strategy in designing functional nanostructures capable of first crossing the BBB and, then, of reaching specific tumor brain cells.

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“…Often, such properties are combined, as recently reported by Perillo et al, who designed multifunctional NP systems composed of superparamagnetic iron oxide NPs that were additionally labeled with a fluorescent dye. Subsequent coating of the surface with a CPP facilitated cell entry in human mammary carcinoma MDA-MB-231 cells [101]. Since the peptide was enriched with an additional cysteine, it could be easily introduced within the PEG shell in a certain orientation-and sequence-specific coupling strategy, being indeed indispensable for the expected biological response.…”

Section: Cancer Imagingmentioning

confidence: 99%

Nanoparticles Modified with Cell-Penetrating Peptides: Conjugation Mechanisms, Physicochemical Properties, and Application in Cancer Diagnosis and Therapy

Gessner

Neundorf

2020

IJMS

144

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Based on their tunable physicochemical properties and the possibility of producing cell-specific platforms through surface modification with functional biomolecules, nanoparticles (NPs) represent highly promising tools for biomedical applications. To improve their potential under physiological conditions and to enhance their cellular uptake, combinations with cell-penetrating peptides (CPPs) represent a valuable strategy. CPPs are often cationic peptide sequences that are able to translocate across biological membranes and to carry attached cargos inside cells and have thus been recognized as versatile tools for drug delivery. Nevertheless, the conjugation of CPP to NP surfaces is dependent on many properties from both individual components, and further insight into this complex interplay is needed to allow for the fabrication of highly stable but functional vectors. Since CPPs per se are nonselective and enter nearly all cells likewise, additional decoration of NPs with homing devices, such as tumor-homing peptides, enables the design of multifunctional platforms for the targeted delivery of chemotherapeutic drugs. In this review, we have updated the recent advances in the field of CPP-NPs, focusing on synthesis strategies, elucidating the influence of different physicochemical properties, as well as their application in cancer research.

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Synthesis and in vitro evaluation of fluorescent and magnetic nanoparticles functionalized with a cell penetrating peptide for cancer theranosis

Cited by 51 publications

References 27 publications

Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes

Targeting HER2-breast tumors with scFv-decorated bimodal nanoprobes

Comparison Between Folic Acid and gH625 Peptide-Based Functionalization of Fe3O4 Magnetic Nanoparticles for Enhanced Cell Internalization

Nanoparticles Modified with Cell-Penetrating Peptides: Conjugation Mechanisms, Physicochemical Properties, and Application in Cancer Diagnosis and Therapy

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