“…Compounds 14–48 were evaluated for their ability to activate PPARs using a GAL4/PPAR chimera system. In these assays, the well-established agonists GW7647 (PPARα), GW501516 (PPARδ), and rosiglitazone (PPARγ) were employed as positive controls, and we found that their EC 50 values were similar to the previously reported ones. − Their maximal responses were observed at 10 nM, 10 nM, and 1 μM, respectively. Thus, referring to the previous reports, PPAR activation by the tested compounds was normalized to the activity of GW7647 at 10 nM (PPARα), GW501516 at 10 nM (PPARδ), and rosiglitazone at 1 μM (PPARγ) to obtain relative activation potency .…”