Synthesis and evaluation of human T cell stimulating activity of an α-sulfatide analogue

Uncoupling between CD1d upregulation induced by retinoic acid and conduritol-B-epoxide and iNKT cell responsiveness

“…Pure CD4 + and CD8 + T cells were obtained from PBL, by positive selection using MACS microbeads and columns (Miltenyi Biotec, Bergisch Gladbach, Germany). Isolation of human iNKT cell clones from PBMC of healthy donors was performed as described before (Franchini et al 2007). The monocytic leukemia cell line THP-1 (ECACC, Salisbury, UK) was kept in culture and grown in RPMI-1640 with Glutamax I complete medium with 1% P/S/A and 10% iFBS, at 37 1C, 5% CO 2 , 99% humidity.…”

Participation of galactosylceramide and sulfatide in glycosynapses between oligodendrocyte or myelin membranes

“…We have examined the effect of silica nanoparticles bearing galactose (Gal‐nanoparticles), galactose‐3‐sulfate (SGal‐nanoparticles), or a combination of galactose and galactose‐3‐sulfate (Gal/SGal‐nanoparticles) on OLs. Propargyl β‐ d ‐3‐sulfogalactose was synthesized by regioselective sulfonation of the dibutylstannylene ketal prepared from propargyl β‐ d ‐galactose [29]. Silica nanoparticles were functionalized with Gal (1.3 μmol galactose/mg nanoparticles, corresponding to complete coverage), SGal (0.8 μmol/mg) and a mixture of Gal/SGal (0.6 μmol Gal + 0.5 μmol SGal/mg) (J. Zhao and A. Basu, unpublished).…”

An efficient and convenient synthesis of deuterium-labelled seminolipid isotopomers and their ESI-MS characterization

“…Selective sulfation (Guilbert et al, 1994) finally gave the target SGG 1a in 70% yield. Selective functionalization at position 3 of galactose was effectively accomplished through reaction of dibutylstannilene acetal with SO 3 ·Me 3 N, as indicated by the downfield shift of the H-3 signal (Franchini et al, 2007) in the 1 H NMR spectrum of SGG 1a.…”