Synthesis and evaluation of carboxymethyl chitosan for glycopeptide enrichment

Bodnar, Edward; Perreault, Hélène

doi:10.1016/j.aca.2015.08.004

Cited by 21 publications

(7 citation statements)

References 48 publications

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“…Most studies on carboxylated chitosan have concerned carboxymethylation reactions [58][59][60]. The steric hindrance effect on the chitosan C 3 -OH group makes the carboxymethylation of the C 3 -OH group more difficult.…”

Section: Carboxylated Chitosanmentioning

confidence: 99%

Chitosan Derivatives and Their Application in Biomedicine

Wang

Meng

et al. 2020

IJMS

530

326

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Chitosan is a product of the deacetylation of chitin, which is widely found in nature. Chitosan is insoluble in water and most organic solvents, which seriously limits both its application scope and applicable fields. However, chitosan contains active functional groups that are liable to chemical reactions; thus, chitosan derivatives can be obtained through the chemical modification of chitosan. The modification of chitosan has been an important aspect of chitosan research, showing a better solubility, pH-sensitive targeting, an increased number of delivery systems, etc. This review summarizes the modification of chitosan by acylation, carboxylation, alkylation, and quaternization in order to improve the water solubility, pH sensitivity, and the targeting of chitosan derivatives. The applications of chitosan derivatives in the antibacterial, sustained slowly release, targeting, and delivery system fields are also described. Chitosan derivatives will have a large impact and show potential in biomedicine for the development of drugs in future.

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Section: Carboxylated Chitosanmentioning

confidence: 99%

Chitosan Derivatives and Their Application in Biomedicine

Wang

Meng

et al. 2020

IJMS

530

326

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“…1548 cm −1 relates to amide NH bending. Primary amine stretch bands are observed around 720 cm −1 . Even with large predominant Si–O stretching bands in both spectra (1100 and 800 cm −1 , respectively), overall the differences indicate functionalization of the beads.…”

Section: Resultsmentioning

confidence: 87%

Multiplexed azido‐group isotopic capture (MAGIC) beads: Selective analysis of azido compounds using a propargyl‐based cleavable linker, a proof of concept

McLellan

Doyle

Bodnar

et al. 2016

Rapid Comm Mass Spectrometry

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This method allows easy identification of captured compounds based on mass and fragmentation analysis. Moreover, it is useful for the analysis of small azide-containing compounds by MALDI-TOF-MS which may not be possible otherwise due to matrix interferences. The insertion of isotopically labeled Arg residues provides the possibility of multiplex analysis, from which the beads have been called MAGIC (for Multiplexed Azido-Group Isotopic Capture). Copyright © 2016 John Wiley & Sons, Ltd.

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“…Lectin‐affinity chromatography is often used for the purpose , although a selective bias to specific structures is pointed out . Carboxymethyl chitosan is used for the enrichment of glycopeptides .…”

Section: Analysis Of N‐ and O‐glycopeptidesmentioning

confidence: 99%

Hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs and therapeutic peptides: A review based on the separation characteristics of the hydrophilic interaction chromatography phases

Ikegami

2019

J of Separation Science

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Applications of hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs, i.e., glycosylated proteins represented by monoclonal antibodies are discussed in the manner of glycoproteomics. They can be analyzed using hydrophilic interaction chromatography in five different stages as (1) their intact forms, (2) their subunits, (3) N‐ and O‐glycopeptides digested by proteases, (4) N‐ and O‐glycans released from the glycoproteins or glycopeptides, and (5) monosaccharides. Hydrophilic interaction chromatography is a more useful tool in the order of (1) to (5). At the stages (4) and (5), quantitation of glycans and saccharides are also reported. Hydrophilic interaction chromatography is employed not only for analytical uses, but also pretreatment items as solid phase extraction, followed by reversed‐phase liquid chromatography separations. Comprehensive search results of these application of hydrophilic interaction chromatography are summarized in tables to show what kind of hydrophilic interaction chromatography columns are suitable for each step of analysis.Relationship of favored and less favored hydrophilic interaction chromatography columns and their separation characteristics such as hydrophilicity, and selectivity for structural difference, is also discussed. Analysis of the therapeutic peptides (not glycosylated) using hydrophilic interaction chromatography is summarized, too.

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Synthesis and evaluation of carboxymethyl chitosan for glycopeptide enrichment

Cited by 21 publications

References 48 publications

Chitosan Derivatives and Their Application in Biomedicine

Chitosan Derivatives and Their Application in Biomedicine

Multiplexed azido‐group isotopic capture (MAGIC) beads: Selective analysis of azido compounds using a propargyl‐based cleavable linker, a proof of concept

Hydrophilic interaction chromatography for the analysis of biopharmaceutical drugs and therapeutic peptides: A review based on the separation characteristics of the hydrophilic interaction chromatography phases

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