Synthesis and Estimation of the Influence of 2,4-Dimethoxyphenylbiguanide on the Glutathione Antioxidant System Activity in Heart and Blood Serum of Rats with Experimental Rheumatoid Arthritis

Сафонова, О. А.; Попова, Т. Н.; Крыльский, Е. Д.; Tanygina, E. S.; Kirilova, E. M.

doi:10.1007/s11094-016-1364-7

Cited by 2 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, RA was confirmed histologically by light microscopic examination of ankle joints in subgroup IIa and progressed after four weeks of RA induction in subgroup IIb. This agreed with previous studies which established that RA developed after two weeks [20] and progressed after four weeks of RA induction [21,22] .…”

Section: Discussionsupporting

confidence: 93%

Evaluation of the possible therapeutic role of omega-3 on ankle joint and lung in a model of rheumatoid arthritis in rats: A histological and immunohistochemical study

Raafat¹,

Hamam²,

Farhan

et al. 2018

Egyptian Journal of Histology

View full text Add to dashboard Cite

Introduction: Rheumatoid arthritis (RA) is a chronic inflammatory systemic autoimmune disease. Lung disease has been recognized as a common complication of RA. Omega-3 is an essential fatty acid with wide range of activity. Aim of the work: To investigate the possible therapeutic role of omega-3 free fatty acid on the structure of ankle joint and lung in a model of adjuvant-induced RA in rats. Materials and Methods: Thirty adult male albino rats were divided into three groups; Group I served as a control group. Group II was used as a model of RA. Rats of this group were given single subcutaneous injection of 0.1ml of complete Freund adjuvant in their hind paws. Group III (omega 3-treated group), omega-3 was given in as a dose of 300 mg/kg orally administered after two weeks of RA induction and continued daily for another two weeks. The ankle joint and the lungs from all groups were dissected out after two and four weeks and processed for light microscopic examination. Histomorphometric studies and statistical analysis were also done. Results: RA was confirmed histologically in the ankle joint after two weeks and was aggravated after four weeks. Erosion of articular cartilage and mononuclear cellular infiltration in the synovium were noticed in both time points. Moreover, the lungs after two weeks showed focal inflammatory cellular infiltration and pulmonary fibrosis that were more evident after four weeks of untreated RA. This was associated with significant increase in the mean area percentage of collagen fibers and number of cells positive for TNF-α immunohistochemical reaction compared to the control group. Omega-3 treated group illustrated improvement of both ankle and lung structures. Conclusion: Omega-3 significantly ameliorated the structural damage of ankle joint and lung after RA induction.

show abstract

Section: Discussionsupporting

confidence: 93%

Evaluation of the possible therapeutic role of omega-3 on ankle joint and lung in a model of rheumatoid arthritis in rats: A histological and immunohistochemical study

Raafat¹,

Hamam²,

Farhan

et al. 2018

Egyptian Journal of Histology

View full text Add to dashboard Cite

show abstract

“…On the 15th day after the resulting inflammation in the absence of treatment, the specific activity of all antioxidant defense enzymes (SOD, catalase, GR, G6PD, LDR directed and LDR reversed) increased, which characterizes their activation as a response to oxidative stress developing against the background of RA. These results are consistent with the studies of the authors, which demonstrated a significant increase in enzyme activity in various tissues (liver, heart, muscles, blood serum), from 1.2 to 1.7 times [48][49][50][51][52].…”

Section: Biological Activity Of the Fa Patch On The Adjuvant-induced ...supporting

confidence: 93%

Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model

Konnova,

Volkov,

Solovyeva

et al. 2023

Sci. Pharm.

View full text Add to dashboard Cite

The formulation of the transdermal patch with fulvic acid (FA) on an emulsion basis using pluronic Kolliphor® p237 as a permeability enhancer was developed and studied for anti-inflammatory properties. FA was isolated from the peat in the Nizhny Novgorod region of Russia and characterized as a potential active pharmaceutical ingredient. In vitro studies of the release of FA from the transdermal patch, as well as the FA penetration through the acetyl cellulose membrane using the Franz diffusion cell, showed its high efficiency (56% and 90%, respectively, in 8 h). In the in vivo experiment, qualitative and quantitative features of the rat knee caused by complete Freund’s adjuvant-induced arthritis (morphological changes, the FA influence on the biochemical indexes) were studied. The inflammatory process that developed within 15 days was accompanied by the activation of antioxidant oxidoreductase enzymes (by 50–70%), the increase in the cross-sectional diameter of the cartilage, and the increase in the values of marker indicators of the process of rheumatoid arthritis. Within 7 days of treatment, under the influence of FA, the values of ESR, RF, leukocytes, C-reactive protein, as well as the biochemical parameters characterizing oxidative stress (SOD, catalase, glutathione reductase, LDH, G6PD) normalized, and the edema reduced. These results may be useful for arthritis treatment using the transdermal patch with FA.

show abstract

Synthesis and Estimation of the Influence of 2,4-Dimethoxyphenylbiguanide on the Glutathione Antioxidant System Activity in Heart and Blood Serum of Rats with Experimental Rheumatoid Arthritis

Cited by 2 publications

References 14 publications

Evaluation of the possible therapeutic role of omega-3 on ankle joint and lung in a model of rheumatoid arthritis in rats: A histological and immunohistochemical study

Evaluation of the possible therapeutic role of omega-3 on ankle joint and lung in a model of rheumatoid arthritis in rats: A histological and immunohistochemical study

Anti-Inflammatory Property Establishment of Fulvic Acid Transdermal Patch in Animal Model

Contact Info

Product

Resources

About