Synthesis and discovery of potent carbonic anhydrase, acetylcholinesterase, butyrylcholinesterase, and α‐glycosidase enzymes inhibitors: The novel N,N′‐bis‐cyanomethylamine and alkoxymethylamine derivatives

Abstract

During this investigation, N,N'-bis-azidomethylamines, N,N'-bis-cyanomethylamine, new alkoxymethylamine and chiral derivatives, which are considered to be a new generation of multifunctional compounds, were synthesized, functional properties were investigated, and anticholinergic and antidiabetic properties of those compounds were studied through the laboratory tests, and it was approved that they contain physiologically active compounds rather than analogues. Novel N-bis-cyanomethylamine and alkoxymethylamine derivatives were effective inhibitors of the α-glycosidase, cytosolic carbonic anhydrase I and II isoforms, butyrylcholinesterase (BChE), and acetylcholinesterase (AChE) with K values in the range of 0.15-13.31 nM for α-glycosidase, 2.77-15.30 nM for human carbonic anhydrase isoenzymes I (hCA I), 3.12-21.90 nM for human carbonic anhydrase isoenzymes II (hCA II), 23.33-73.23 nM for AChE, and 3.84-48.41 nM for BChE, respectively. Indeed, the inhibition of these metabolic enzymes has been considered as a promising factor for pharmacologic intervention in a diversity of disturbances.

Novel amides of 1,1‐bis‐(carboxymethylthio)‐1‐arylethanes: Synthesis, characterization, acetylcholinesterase, butyrylcholinesterase, and carbonic anhydrase inhibitory properties

“…[30] The amount of eluted protein was quantitated spectrophotometrically at 280 nm. [32] This biochemical method was established with 10% and 3% acrylamide for the running and stacking gels, respectively. [32] This biochemical method was established with 10% and 3% acrylamide for the running and stacking gels, respectively.…”

Some pyrazoles derivatives: Potent carbonic anhydrase, α‐glycosidase, and cholinesterase enzymes inhibitors

“…Cholinesterase (ChE) hydrolyzes the choline esters. In mammalians, there are two major forms of cholinesterases, namely, butyrylcholinesterase (BChE) and acetylcholinesterase (AChE), characterized by very high concentrations in the nerve cells, muscle, placental tissue, erythrocytes, and brain . AChE is a specific choline esterase and also is a hydrolytic enzyme of the serine class.…”

The effects of zingerone against vancomycin‐induced lung, liver, kidney and testis toxicity in rats: The behavior of some metabolic enzymes

“…CA, AChE, and BChE enzymes are the essential biological molecules needed for metabolic processes in all living cells. CA enzyme is involved in many physiological and biochemical processes including pH regulation, fluid secretion, acid/base balance, calcification, ion exchange, and ureagenesis . In addition, increased AChE and BChE enzyme activities are associated with impairment of cholinergic neurons .…”