Synthesis and characterization of ZnO nanomaterial from Cassia sieberiana and determination of its anti-inflammatory, antioxidant and antimicrobial activities

Kyene, Michael Odoi; Droepenu, Eric Kwabena; Ayertey, Frederick; Yeboah, Genevieve Naana; Archer, Mary-Ann; Kumadoh, Doris; Mintah, Susana Oteng; Gordon, Paa Kwesi; Appiah, Alfred Ampomah

doi:10.1016/j.sciaf.2022.e01452

Cited by 12 publications

(2 citation statements)

References 32 publications

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“…Antioxidant capacity of the biogenic ZnO NPs was evaluated using the 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) radical scavenging assay with slight modification (Kyene et al, 2023). Ascorbic acid was used as a reference for comparison of the DPPH assay results.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Evaluation of the cytotoxicity, antioxidant activity, and molecular docking of biogenic zinc oxide nanoparticles derived from pumpkin seeds

Kadir,

Murugan,

Khan

et al. 2023

Microscopy Res & Technique

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This study aimed to investigate the characterization of zinc oxide nanoparticles (ZnONPs) produced from Cucurbita pepo L. (pumpkin seeds) and their selective cytotoxic effectiveness on human colon cancer cells (HCT 116) and African Green Monkey Kidney, Vero cells. The study also investigated the antioxidant activity of ZnONPs. The study also examined ZnONPs' antioxidant properties. This was motivated by the limited research on the comparative cytotoxic effects of ZnO NPs on normal and HCT116 cells. The ZnO NPs were characterized using Fourier‐transform infrared spectroscopy (FTIR), Thermogravimetric Analysis (TGA), Transmission Electron Microscope/Selected Area Electron Diffraction (TEM/SAED), and Scanning Electron Microscope‐Energy Dispersive X‐ray (SEM–EDX) for determination of chemical fingerprinting, heat stability, size, and morphology of the elements, respectively. Based on the results, ZnO NPs from pumpkins were found to be less than 5 μm and agglomerates in nature. Furthermore, the ZnO NPs fingerprinting and SEM–EDX element analysis were similar to previous literature, suggesting the sample was proven as ZnO NPs. The ZnO NPs also stable at a temperature of 380°C indicating that the green material is quite robust at 60–400°C. The cell viability of Vero cells and HCT 116 cell line were measured at two different time points (24 and 48 h) to assess the cytotoxicity effects of ZnO NP on these cells using AlamarBlue assay. Cytotoxic results have shown that ZnO NPs did not inhibit Vero cells but were slightly toxic to cancer cells, with a dose–response curve IC50 = ~409.7 μg/mL. This green synthesis of ZnO NPs was found to be non‐toxic to normal cells but has a slight cytotoxicity effect on HCT 116 cells. A theoretical study used molecular docking to investigate nanoparticle interaction with cyclin‐dependent kinase 2 (CDK2), exploring its mechanism in inhibiting CDK2's role in cancer. Further study should be carried out to determine suitable concentrations for cytotoxicity studies. Additionally, DPPH has a significant antioxidant capacity, with an IC50 of 142.857 μg/mL.Research Highlights Pumpkin seed extracts facilitated a rapid, high‐yielding, and environmentally friendly synthesis of ZnO nanoparticles. Spectrophotometric analysis was used to investigate the optical properties, scalability, size, shape, dispersity, and stability of ZnO NPs. The cytotoxicity of ZnO NPs on Vero and HCT 116 cells was assessed, showing no inhibition of Vero cells and cytotoxicity of cancer cells. The DPPH assay was also used to investigate the antioxidant potential of biogenic nanoparticles. A molecular docking study was performed to investigate the interaction of ZnO NPs with CDK2 and to explore the mechanism by which they inhibit CDK2's role in cancer.

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Section: Antioxidant Activitymentioning

confidence: 99%

Evaluation of the cytotoxicity, antioxidant activity, and molecular docking of biogenic zinc oxide nanoparticles derived from pumpkin seeds

Kadir,

Murugan,

Khan

et al. 2023

Microscopy Res & Technique

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“…ZnO nanoparticles have also shown promising antioxidant activities. ZnO nanoparticles which were synthesized utilizing Cassia sieberiana 's methanolic root bark extract and the ZnO nanoparticles synthesized by Pichia kudriavzevii Yeast Strain have demonstrated potent antioxidant properties against the DPPH free radical scavenging assay 39 . However, the biocompatibility of those nanomaterials remains the challenge demarcating applicability of them in the biological systems.…”

Section: Introductionmentioning

confidence: 99%

In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth

Jayanetti,

Thambiliyagodage,

Liyanaarachchi

et al. 2024

Sci Rep

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Polyethyleneglycol-coated biocompatible CuO–ZnO nanocomposites were fabricated hydrothermally varying Zn:Cu ratios as 1:1, 2:1, and 1:2, and their antibacterial activity was determined through the well diffusion method against the Gram-negative Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, and the Gram-positive Staphylococcus aureus. The minimum inhibitory concentration and the minimum bactericidal concentration values of the synthesized samples were determined. Subsequently, the time synergy kill assay was performed to elucidate the nature of the overall inhibitory effect against the aforementioned bacterial species. The mean zone of inhibition values for all four samples are presented. The inhibitory effect increased with increasing concentration of the nanocomposite (20, 40 and 60 mg/ml) on all the bacterial species except for S. aureus. According to the MBC/MIC ratio, ZnO was found to be bacteriostatic for E. coli and P. aeruginosa, and bactericidal for S. aureus and K. pneumoniae. Zn:Cu 2:1 was bactericidal on all bacterial species. A bacteriostatic effect was observed on E. coli and P. aeruginosa in the presence of Zn:Cu 1:1 whereas, it showed a bactericidal effect on S. aureus and K. pneumoniae. Zn:Cu 1:2 exhibited a bacteriostatic effect on E. coli while a bactericidal effect was observed for E. coli, P. aeruginosa, and K. pneumoniae. The metal oxide nanocomposites were found to be more sensitive towards the Gram-positive strain than the Gram-negative strains. Further, all the nanocomposites possess anti-oxidant activity as shown by the DPPH assay.

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ZnO Nanoparticles: Sustainable Plant Production

Mandal

2023

Nanotechnology in the Life Sciences

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Synthesis and characterization of ZnO nanomaterial from Cassia sieberiana and determination of its anti-inflammatory, antioxidant and antimicrobial activities

Cited by 12 publications

References 32 publications

Evaluation of the cytotoxicity, antioxidant activity, and molecular docking of biogenic zinc oxide nanoparticles derived from pumpkin seeds

Evaluation of the cytotoxicity, antioxidant activity, and molecular docking of biogenic zinc oxide nanoparticles derived from pumpkin seeds

In vitro influence of PEG functionalized ZnO–CuO nanocomposites on bacterial growth

ZnO Nanoparticles: Sustainable Plant Production

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