Synthesis and characterization of Zinc/Chitosan-Folic acid complex

Bandara, Subhani; Carnegie, Codi anne; Johnson, Chevaun; Akindoju, Feyisayo; Williams, Ebonee; Swaby, Julia M.; Oki, Aderemi; Carson, Laura

doi:10.1016/j.heliyon.2018.e00737

Cited by 47 publications

(26 citation statements)

References 41 publications

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“…This may be due to the presence of residual free carboxylic acid groups (−COO -) of FA. Similar findings were previously mentioned for shifting the negative values of the zeta potential toward the positive one for different nanoparticles after FA conjugation [15,[69][70][71].…”

Section: Discussionsupporting

confidence: 88%

Induction of mitochondria mediated apoptosis in human ovarian cancer cells by folic acid coated tin oxide nanoparticles

Hanna

Saad

2021

PLoS ONE

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Purpose This study aims to prepare folic acid coated tin oxide nanoparticles (FA-SnO2 NPs) for specifically targeting human ovarian cancer cells with minimum side effects against normal cells. Methods The prepared FA-SnO2 NPs were characterized by FT-IR, UV-vis spectroscopy, XRD, SEM and TEM. The inhibition effects of FA-SnO2 NPs against SKOV3 cancer cell were tested by MTT and LDH assay. Apoptosis induction in FA-SnO2 NPs treated SKOV3 cells were investigated using Annexin V/PI, AO/EB and Comet assays and the possible mechanisms of the cytotoxic action were studied by Flow cytometry, qRT-PCR, Immunohistochemistry, and Western blotting analyses. The effects of FA-SnO2 NPs on reactive oxygen species generation in SKOV3 cells were also examined. Additionally, the safety of utilization FA-SnO2 NPs were studied in vivo using Wister rats. Results The obtained FA-SnO2 NPs displayed amorphous spherical morphology with an average diameter of 157 nm and a zeta potential value of -24 mV. Comparing to uncoated SnO2 NPs, FA-SnO2 NPs had a superior inhibition effect towards SKOV3 cell growth that was suggested to be mediated through higher reactive oxygen species generation. It was showed that FA-SnO2 NPs increased significantly the % of apoptotic cells in the sub- G1 and G2/M phases with a higher intensity comet nucleus in SKOV3 treated cells. Furthermore, FA-SnO2 NPs was significantly increased the expression levels of P53, Bax, and cleaved Caspase-3 and accompanied with a significant decrease of Bcl-2 in the treated SKOV3 cells. Conclusion Overall, the results suggested that an increase in cellular FA-SnO2 NPs internalization resulted in a significant induced cytotoxicity in SKOV3 cancer cells in dose-dependent mode through ROS-mediated cell apoptosis that may have occurred through mitochondrial pathway. Additionally, the results confirmed the safety of utilization FA-SnO2 NPs against living systems. So, FA-SnO2 NPs with a specific targeting moiety may be a promising therapeutic candidate for human ovarian cancer.

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Section: Discussionsupporting

confidence: 88%

Induction of mitochondria mediated apoptosis in human ovarian cancer cells by folic acid coated tin oxide nanoparticles

Hanna

Saad

2021

PLoS ONE

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“…The peak at 1289/1296 cm −1 refers to the C–N stretching vibration of the benzenoid unit. The presence of this peak further indicates that there was an occurrence of π-electron delocalization in the polymer chain due to the protonation process during polymerization [28]. The characteristic band observed at 1444/1448 cm −1 corresponds to the C=C and N=N stretching vibrations and the C–H bending vibration in the benzenoid ring of the PAni structure, respectively.…”

Section: Resultsmentioning

confidence: 99%

Synthesis and Characterization of Acetic Acid-Doped Polyaniline and Polyaniline–Chitosan Composite

et al. 2019

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Polyaniline–chitosan (PAni–Cs) composite films were synthesized using a solution casting method with varying PAni concentrations. Polyaniline powders used in the composite synthesis were polymerized using acetic acid as the dopant media. Raman spectroscopy revealed that the PAni powders synthesized using hydrochloric acid and acetic acid did not exhibit significant difference to the chemical features of PAni, implying that PAni was formed in varying concentrations of the dopant media. The presence of agglomerated particles on the surface of the Cs composite, which may have been due to the presence of PAni powders, was observed with scanning electron microscope–energy dispersive X-ray spectroscopy (SEM–EDX). Ultraviolet–visible (UV–Vis) spectroscopy further showed the interaction of PAni with Cs where the Cs characteristic peak shifted to a higher wavelength. Cell viability assay also revealed that the synthesized PAni–Cs composites were nontoxic and may be utilized for future biomedical applications.

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“…Bands between 1154 cm −1 and 1027 cm −1 are assigned to C-O-C of the glycosidic bonds. The bands observed in the 1155 cm −1 and 800 cm −1 are known to be C-O stretch vibrations of chitosan [22].…”

Section: Physicochemical and Structural Characterization Of Modified Chitosan Filmsmentioning

confidence: 99%

Zinc Oxide and Copper Chitosan Composite Films with Antimicrobial Activity

et al. 2021

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The role of the oral microbiome and its effect on dental diseases is gaining interest. Therefore, it has been sought to decrease the bacterial load to fight oral cavity diseases. In this study, composite materials based on chitosan, chitosan crosslinked with glutaraldehyde, chitosan with zinc oxide particles, and chitosan with copper nanoparticles were prepared in the form of thin films, to evaluate a new alternative with a more significant impact on the oral cavity bacteria. The chemical structures and physical properties of the films were characterized using by Fourier transform infrared spectroscopy (FTIR,) Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), elemental analysis (EDX), thermogravimetric analysis (TGA), X-ray diffraction (XRD), scanning electron microscopy (SEM), and contact angle measurements. Subsequently, the antimicrobial activity of each material was evaluated by agar diffusion tests. No differences were found in the hydrophilicity of the films with the incorporation of ZnO or copper particles. Antimicrobial activity was found against S. aureus in the chitosan film crosslinked with glutaraldehyde, but not in the other compositions. In contrast antimicrobial activity against S. typhimurium was found in all films. Based on the data of present investigation, chitosan composite films could be an option for the control of microorganisms with potential applications in various fields, such as medical and food industry.

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Synthesis and characterization of Zinc/Chitosan-Folic acid complex

Cited by 47 publications

References 41 publications

Induction of mitochondria mediated apoptosis in human ovarian cancer cells by folic acid coated tin oxide nanoparticles

Induction of mitochondria mediated apoptosis in human ovarian cancer cells by folic acid coated tin oxide nanoparticles

Synthesis and Characterization of Acetic Acid-Doped Polyaniline and Polyaniline–Chitosan Composite

Zinc Oxide and Copper Chitosan Composite Films with Antimicrobial Activity

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